Ankara Üniversitesi Veteriner Fakültesi Dergisi
 
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İnfeksiyöz bursal hastalığın immunoperoksidaz tekniği ile tanısı

Yıl 2004, Cilt: 51 Sayı: 3, 189 - 193, 01.09.2004

Tolga GÜVENÇ Rıfkı HAZIROĞLU Murat YARIM Recai TUNCA

Öz

İnfeksiyöz bursal hastalığın tanısı, immunoperoksidaz metodu kullanılarak doğal olarak infekte 21 günlük civcivlerde incelendi. Viral antijenler bursa Fabricius’daki makrofaj ve lenfositlerde gözlendi. Çalışmada kullanılan metodun duyarlı ve spesifik olduğu ve hastalığın tanısında kullanılabileceği saptandı

Anahtar Kelimeler

İmmunohistokimya, infeksiyöz bursal hastalık, poliklonal antikor

Kaynakça

  • Cruz-Coy JS, Giambrone JJ, Hoerr FJ (1993): İmmu- nohistochemical detection of infectious bursal disease vi- rus in formalin-fixed, paraffin-embedded chicken tissues using monoclonal antibody. Avian Dis, 37, 577-581.
  • Eterradosi A, Picault J, Drouin P (1992): Pathogenicity and preliminary antigenic characterisation of six infec- tious bursal disease virus strains isolated in France from acute outbreaks. J Vet Med, 39, 683-691.
  • Fernandes A, Martin M, Sierra M (1989): Immunohis- tological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius. Dtsch Tierarzl Wschr, 96, 157-160.
  • Haziroglu R, Maeda M, Nakamura K, Haritani M, Narita M (1988): Diagnosis of infectious bursal disease (IBD) by immunofluoresence. Ankara Univ Vet Fak Derg, 25, 289-298.
  • Jonson L, Engstrom B (1986): Immunocytochemical detection of infectious bursal disease and infectious bron- chitis viral antigens in fixed, paraffin embedded chicken tissues. Avian Pathol, 15, 385-393.
  • Kovacevic SA, Gagic M, Lazic S, Kovacevic M (1999): Immunohistochemical detection of infectious bursal dis- ease virus antigen in the bursa of fabricius of experimen- tally infected chickens. Acta Vet. (Beograd), 49, 13-20.
  • Lukert PD, Saif YM (1997): Infectious bursal disease. 721-738. In: BW Calnek, H J Barnes, CW Beard, McDou- gald L R, Saif M Y (Eds.): Disease of Poultry. Iowa State University Press, Ames, Iowa.
  • Tanimura N, Tsukamoto K, Nakamura, K, Narita M, Maeda M (1995): Association between pathogenicity of infectious bursal disease virus and viral antigen distribu- tion detected by immunohistochemistry. Avian Dis, 39, 9- 20. Geliş tarihi: 31.10.2003 / Kabul tarihi: 16.02.2004

Diagnosis of infectious bursal disease by immunoperoxidase technique

Yıl 2004, Cilt: 51 Sayı: 3, 189 - 193, 01.09.2004

Tolga GÜVENÇ Rıfkı HAZIROĞLU Murat YARIM Recai TUNCA

Öz

The diagnosis of infectious bursal disease of naturally infected 21-day-old chickens was investigated with immunoperoxidase method. Viral antigens were observed in the macrophages and lymphocytes of the bursa of Fabricius. It was observed that the method was sensitive and specific and it could be used in the diagnosis of this disease

Anahtar Kelimeler

Immunohistochemistry, infectious bursal disease, polyclonal antibody

Kaynakça

  • Cruz-Coy JS, Giambrone JJ, Hoerr FJ (1993): İmmu- nohistochemical detection of infectious bursal disease vi- rus in formalin-fixed, paraffin-embedded chicken tissues using monoclonal antibody. Avian Dis, 37, 577-581.
  • Eterradosi A, Picault J, Drouin P (1992): Pathogenicity and preliminary antigenic characterisation of six infec- tious bursal disease virus strains isolated in France from acute outbreaks. J Vet Med, 39, 683-691.
  • Fernandes A, Martin M, Sierra M (1989): Immunohis- tological identification of both infectious bursal and Marek virus antigens in the bursa of Fabricius. Dtsch Tierarzl Wschr, 96, 157-160.
  • Haziroglu R, Maeda M, Nakamura K, Haritani M, Narita M (1988): Diagnosis of infectious bursal disease (IBD) by immunofluoresence. Ankara Univ Vet Fak Derg, 25, 289-298.
  • Jonson L, Engstrom B (1986): Immunocytochemical detection of infectious bursal disease and infectious bron- chitis viral antigens in fixed, paraffin embedded chicken tissues. Avian Pathol, 15, 385-393.
  • Kovacevic SA, Gagic M, Lazic S, Kovacevic M (1999): Immunohistochemical detection of infectious bursal dis- ease virus antigen in the bursa of fabricius of experimen- tally infected chickens. Acta Vet. (Beograd), 49, 13-20.
  • Lukert PD, Saif YM (1997): Infectious bursal disease. 721-738. In: BW Calnek, H J Barnes, CW Beard, McDou- gald L R, Saif M Y (Eds.): Disease of Poultry. Iowa State University Press, Ames, Iowa.
  • Tanimura N, Tsukamoto K, Nakamura, K, Narita M, Maeda M (1995): Association between pathogenicity of infectious bursal disease virus and viral antigen distribu- tion detected by immunohistochemistry. Avian Dis, 39, 9- 20. Geliş tarihi: 31.10.2003 / Kabul tarihi: 16.02.2004

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Tolga GÜVENÇ
Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey

Rıfkı HAZIROĞLU
Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey

Murat YARIM
Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey

Recai TUNCA
Faculty of Veterinary Medicine, University of Ondokuz Mayıs, Samsun, Turkey

Yayımlanma Tarihi 1 Eylül 2004
Yayınlandığı Sayı Yıl 2004Cilt: 51 Sayı: 3

Kaynak Göster

Bibtex @ { auvfd600094, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2004}, volume = {51}, number = {3}, pages = {189 - 193}, title = {Diagnosis of infectious bursal disease by immunoperoxidase technique}, key = {cite}, author = {Güvenç, Tolga and Hazıroğlu, Rıfkı and Yarım, Murat and Tunca, Recai} }
APA Güvenç, T. , Hazıroğlu, R. , Yarım, M. & Tunca, R. (2004). Diagnosis of infectious bursal disease by immunoperoxidase technique . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 51 (3) , 189-193 . Retrieved from http://vetjournal.ankara.edu.tr/tr/pub/issue/47522/600094
MLA Güvenç, T. , Hazıroğlu, R. , Yarım, M. , Tunca, R. "Diagnosis of infectious bursal disease by immunoperoxidase technique" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 51 (2004 ): 189-193 <http://vetjournal.ankara.edu.tr/tr/pub/issue/47522/600094>
Chicago Güvenç, T. , Hazıroğlu, R. , Yarım, M. , Tunca, R. "Diagnosis of infectious bursal disease by immunoperoxidase technique". Ankara Üniversitesi Veteriner Fakültesi Dergisi 51 (2004 ): 189-193
RIS TY - JOUR T1 - İnfeksiyöz bursal hastalığın immunoperoksidaz tekniği ile tanısı AU - TolgaGüvenç, RıfkıHazıroğlu, MuratYarım, RecaiTunca Y1 - 2004 PY - 2004 N1 - DO - T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 189 EP - 193 VL - 51 IS - 3 SN - 1300-0861-1308-2817 M3 - UR - Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Diagnosis of infectious bursal disease by immunoperoxidase technique %A Tolga Güvenç , Rıfkı Hazıroğlu , Murat Yarım , Recai Tunca %T Diagnosis of infectious bursal disease by immunoperoxidase technique %D 2004 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 51 %N 3 %R %U
ISNAD Güvenç, Tolga , Hazıroğlu, Rıfkı , Yarım, Murat , Tunca, Recai . "Diagnosis of infectious bursal disease by immunoperoxidase technique". Ankara Üniversitesi Veteriner Fakültesi Dergisi 51 / 3 (Eylül 2004): 189-193 .
AMA Güvenç T. , Hazıroğlu R. , Yarım M. , Tunca R. Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Univ Vet Fak Derg. 2004; 51(3): 189-193.
Vancouver Güvenç T. , Hazıroğlu R. , Yarım M. , Tunca R. Diagnosis of infectious bursal disease by immunoperoxidase technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2004; 51(3): 189-193.
IEEE T. Güvenç , R. Hazıroğlu , M. Yarım ve R. Tunca , "Diagnosis of infectious bursal disease by immunoperoxidase technique", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 51, sayı. 3, ss. 189-193, Eyl. 2004