Öz
Experimental candidiasis was performed via different routes in guinea pigs immunosuppressed with cylophosphamide and dexamethasone by administrating C. albicans ATCC 26555 serotype A strain. Three experimental groups were constituted by administrating agent through intravenous (Group I, n: 12), intraperitoneal (Group 2, n: 12) and oral (group 3, n: i2) routes. Four guinea pigs for eaeh group were used to set up control (Group 4, n: i2) and physiologic saline solution was injected same routes as experimental groups. The guinea pigs have died in Group i and Group 2, at the percent of 58,3 % and 41,6 %, respectively. However, mortality was not observed in Group 3. The lesions were different in location depending on the inoculation routes. Macroscopieally, multifocal gray to white foci were observed intensively in the lung,liver, heart, spleen and kidney in Group 1. In Group 2, the macroscopical lesions were generally seen in the abdominal cavity organs and testicles. However, in Group 3, the lesions were observed usually on the upper digestive tracts. Microscopically, systemic infection characterized by pyogranulomatous lesions was observed in Group i and Group 2. In addition the similar lesions were seen in the testicles of Group 2. In Group 3, local infection characterized by hyperkeratosis of the squamous epithelium and uleers covered with pseudomembranes was occuned. Histochemically, blastospores, pseudohyphae and hyphae were see n in the all lesions.
Anahtar Kelimeler
Candida albicans experimentalcandidiasis guinea pig pathology
Kaynakça
- Andrutis KA, Riggle PJ, Kumamoto CA, Trzipori S (2000): Intestinallesionsassociated with dissemined can- didiasis Mierobiol,38, 2317-2323. animal modeU Clin
- Arda M (1980)Mikoloji.Ankara Üniv Yet Fak Yayınları
- Ankara Üniversitesi Basımevi, Ankara. s.: 206-214. Asrani RK, Gupta RKP, Sadana JK, Pandita A (1993)
- Experimental candidiasis in Japanse quail: pathological changes,Myeopathol, 121,83-89. Aydemirş,Tümbay E (I 999):Histopatolojik preparasy- onlarda mantarlarm Hastalıkları ve Klinik Mikoloji Kongresi, Tutanaklar. E. Tümbay, E., İnci, R., Hilmioğlu, S., Aydemir,Ş(Ed), Ege Üniversitesi Basımevi, İzmir. 67-75. i Ulusal Mantar Cole GT, Lynn KT,Seshan KR (1990):An animal model for oropharyngeal,esophageal and gastric candidosis. Myeoses, 33, 7-19.
- Cole GT, Lynn KT, Seshan KR (1990):Evaluation of a murine model of hepatic candidiasis. JClin Mierobiol, 28, 1841.
- Cole GT, Lynn KT, Seshan KR, Pope LM (1989)
- Gastointestinal and systemic candidosis in immunocom- primised mice. JMed Yet Myeol,27, 363-380. Cole GT, Seshan KR, Pope LM, Yancey RJ (1988): Morphological
- Candida albicaııs in the infant mouse JMed et Myeol,26, 185. Cutsem JV, Gerven FV, Fransen J,Jansen PAJ (1991)
- Experimental Candidosis in Animals and Chemotherapy. 115. In: E Tümbay, HRP Seeliger, O Anğ (Ed), Candida and Candidamyeosis, Plenum Press. New York. LO.Douglas LJ (1991):Adhesioıı ofCandida albicans to Host
- Surfaces.43-47. In: E Tümbay, HRP Seeliger, O Anğ (Ed), Candida and Candidamyeosis, Plenum Press. New York. Ekenna O, Sherertz RJ (1987):Factors affectiııg colo- nization and dissemination of Candida albicans from the gastoi11lestinaltract of miceInfeet Immun, 55, 1558
- Hazıroğlu R, Diker KS, Arda M (1991):Pathologyof Systemic Candidiasis pseudotropicalis.237-240. In: E Tümbay, HRP Seeliger
- O Anğ (Ed), Candida and Candidamyeosis, Plenum Press. New York. hıduced by Candida Hurley DL, Fauci AS (1975):Disseminated candidiasis.
- I. an experimental model in the guinea pig. JInfeet Dis, 516-521. Kretschmar M, Hube B, Bertsch T, Sanglard D, Merker R, Schroder M, Hof H, Nichterlein T (I 999): Gemı tubes and proteinase activity contrubute to viru- lence of Candida albicans in murine peritonitis.Infeet Immun, 67, 6637-6642.
- Kuştimur S (1994):Kandida patogenezinde roloynayan faktörler.Mikrobiyol Bült, 28, 175-181. l LK.(l991).
- Factors of. the Pathogenicity of Candida albicans- A Reviev. 49-53. In: E Tümbay, HRP Seeliger, Plenum Press. New York. ÜH,Hazıroğlu and Candidamycosis, Milli R (2001): Veteriner Patoloji.
- Tamer Mat Yayıncılık Ankara.ı.Cilt: s. 3, 114. Ochiai B, Valentine BA, AltschulM (2000): ntestinal candidiasis in a dog. Yet Rec, 146,228-229. Schreibman Techniques Johns Tissue (1997): Hopkins University Animal
- Press. Ltd. 5. Edition. p. 312-317. F, Fernandez Rodriguez Jensen puppy associated with parvoviralinfection. Yet Rec, 142, 466. A, Monteros AE, Wohlsein P, candidiasisin a HE (1998): Acute disseminated Schmidt A.(1995): Systeıııic candidiasis in Sprague
- Dawley rats. J Med Yet Mycol, 34, 99- 104. ı q991) Seeliger HPR, Patzelt C Host-[parasite nteraction-Mechanisms of Pathogenesis. 59.In: E i O Anğ(Ed), Tümbay, HRPSeeliger, Canqida and Candidamycosis, Plenum Press. Ne:w York. i Geliş Tarihi 23.07.2003 Kabul Tarihi 29.07.2003j
Öz
Çalışmada, dekzametazon ve siklofosfamid ile bağışıklık sistemi baskılanan erkek kobaylara farklı yollarla C. albicans serotip A (ATCC 26555) suşu verilerek deneysel kandidiazis oluşturuldu. Hayvanlar intravenöz (Grup i,n: 12), intraperitoneal (Grup 2, n:12) ve oral (Grup 3, n:12) yolla enfekte edildi. Kontrololarak (Grup 4, n:12) her deney grubu için 4 kobaya aynı yollarla fizyolojik tuzlu su verildi. İnokülasyon sonrası kobayların Grup i'de % 58,3 'ü, Grup 2'de % 41 ,6'sl öldü. Buna karşın Grup 3'teki kobaylarda ölüm şekillenmedi. Enfeksiyonda şekillenen lezyonların dağılımı etkenin veriliş yoluna göre farklılık gösterdi. Makroskobik olarak Grup i'de çoğunlukla akciğer, karaciğer, kalp, dalak ve böbrekte çok sayıda boz beyaz renkte odaklar şekillendi. Grup 2'de makroskobik lezyonlara çoğunlukla karın boşluğu organları ve testislerde rastlandı. Grup 3 'te ise lezyonlar çoğunlukla üst sindirim sisteminde dikkati çekti. Histopatolojik olarak Grup i ve 2'de çeşitli organlarda pyogranülomatöz lezyonlarla karakterize sistemik enfeksiyon oluştu. Ayrıca, Grup 2'de testislerde de lezyonlara rastlandı. Grup 3'te ise çoğunlukla üst sindirim sisteminde keratinize epitelde keratin fazlalaşması ve üzeri yalancı membranla örtülü ülserlerIe karakterize yersel enfeksiyon gözlendi. Histokimyasal olarak tüm lezyonlarda, blastospor, yalancı ve gerçek hifalar saptandı
Anahtar Kelimeler
Kaynakça
- Andrutis KA, Riggle PJ, Kumamoto CA, Trzipori S (2000): Intestinallesionsassociated with dissemined can- didiasis Mierobiol,38, 2317-2323. animal modeU Clin
- Arda M (1980)Mikoloji.Ankara Üniv Yet Fak Yayınları
- Ankara Üniversitesi Basımevi, Ankara. s.: 206-214. Asrani RK, Gupta RKP, Sadana JK, Pandita A (1993)
- Experimental candidiasis in Japanse quail: pathological changes,Myeopathol, 121,83-89. Aydemirş,Tümbay E (I 999):Histopatolojik preparasy- onlarda mantarlarm Hastalıkları ve Klinik Mikoloji Kongresi, Tutanaklar. E. Tümbay, E., İnci, R., Hilmioğlu, S., Aydemir,Ş(Ed), Ege Üniversitesi Basımevi, İzmir. 67-75. i Ulusal Mantar Cole GT, Lynn KT,Seshan KR (1990):An animal model for oropharyngeal,esophageal and gastric candidosis. Myeoses, 33, 7-19.
- Cole GT, Lynn KT, Seshan KR (1990):Evaluation of a murine model of hepatic candidiasis. JClin Mierobiol, 28, 1841.
- Cole GT, Lynn KT, Seshan KR, Pope LM (1989)
- Gastointestinal and systemic candidosis in immunocom- primised mice. JMed Yet Myeol,27, 363-380. Cole GT, Seshan KR, Pope LM, Yancey RJ (1988): Morphological
- Candida albicaııs in the infant mouse JMed et Myeol,26, 185. Cutsem JV, Gerven FV, Fransen J,Jansen PAJ (1991)
- Experimental Candidosis in Animals and Chemotherapy. 115. In: E Tümbay, HRP Seeliger, O Anğ (Ed), Candida and Candidamyeosis, Plenum Press. New York. LO.Douglas LJ (1991):Adhesioıı ofCandida albicans to Host
- Surfaces.43-47. In: E Tümbay, HRP Seeliger, O Anğ (Ed), Candida and Candidamyeosis, Plenum Press. New York. Ekenna O, Sherertz RJ (1987):Factors affectiııg colo- nization and dissemination of Candida albicans from the gastoi11lestinaltract of miceInfeet Immun, 55, 1558
- Hazıroğlu R, Diker KS, Arda M (1991):Pathologyof Systemic Candidiasis pseudotropicalis.237-240. In: E Tümbay, HRP Seeliger
- O Anğ (Ed), Candida and Candidamyeosis, Plenum Press. New York. hıduced by Candida Hurley DL, Fauci AS (1975):Disseminated candidiasis.
- I. an experimental model in the guinea pig. JInfeet Dis, 516-521. Kretschmar M, Hube B, Bertsch T, Sanglard D, Merker R, Schroder M, Hof H, Nichterlein T (I 999): Gemı tubes and proteinase activity contrubute to viru- lence of Candida albicans in murine peritonitis.Infeet Immun, 67, 6637-6642.
- Kuştimur S (1994):Kandida patogenezinde roloynayan faktörler.Mikrobiyol Bült, 28, 175-181. l LK.(l991).
- Factors of. the Pathogenicity of Candida albicans- A Reviev. 49-53. In: E Tümbay, HRP Seeliger, Plenum Press. New York. ÜH,Hazıroğlu and Candidamycosis, Milli R (2001): Veteriner Patoloji.
- Tamer Mat Yayıncılık Ankara.ı.Cilt: s. 3, 114. Ochiai B, Valentine BA, AltschulM (2000): ntestinal candidiasis in a dog. Yet Rec, 146,228-229. Schreibman Techniques Johns Tissue (1997): Hopkins University Animal
- Press. Ltd. 5. Edition. p. 312-317. F, Fernandez Rodriguez Jensen puppy associated with parvoviralinfection. Yet Rec, 142, 466. A, Monteros AE, Wohlsein P, candidiasisin a HE (1998): Acute disseminated Schmidt A.(1995): Systeıııic candidiasis in Sprague
- Dawley rats. J Med Yet Mycol, 34, 99- 104. ı q991) Seeliger HPR, Patzelt C Host-[parasite nteraction-Mechanisms of Pathogenesis. 59.In: E i O Anğ(Ed), Tümbay, HRPSeeliger, Canqida and Candidamycosis, Plenum Press. Ne:w York. i Geliş Tarihi 23.07.2003 Kabul Tarihi 29.07.2003j
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 2004 |
| Yayımlandığı Sayı | Yıl 2004Cilt: 51 Sayı: 2 |