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İnsan ve rat karaciğer primer hücre kültürlerinde farklı N-nitroso bileşiklerinin DNA üzerindeki etkileri

Yıl 2002, Cilt: 49 Sayı: 1, 23 - 29, 01.03.2002

Taner Pamukçu Alexandre Scott

https://doi.org/10.1501/Vetfak_0000001641

Öz

Kaynakça

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The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA

Yıl 2002, Cilt: 49 Sayı: 1, 23 - 29, 01.03.2002

Taner Pamukçu Alexandre Scott

https://doi.org/10.1501/Vetfak_0000001641

Öz

Ten earcinogenic N-nitroso eompounds were examined for DNA-damaging activity in prımary cultures of human
'1nd rat h,:patocytes. DNA fragmentation was mcasured hy the alkaline dution techniClue. and unscheduled Df\A synthesis was by
qu,ıntitatiıe mıtoradiography. Positive dose-related responses in the range of subtoxic eoncentrations indicated were obtained ın cclls
of hoth species with N.nitrosodiethylanııne (I 0-32 mM). :"J-nitrosodi-n-propylamine (ı .s-l O mM). N-nitrosomorpholine (i -3.2 mM).
N nıtroscpıperidine (1-3.2 mM). N-nitrosopyrrolidine (3.2-1 S mM). N-nitroso-N-methylurca (O.32-I.S mM). N-nitroso-i\ .ethylurea
(0.32-1.8 mM) and N-nitroso-f\hutylurea (O. i-0.32 mM). N-nitrosodi-n-hutylamine was practicaııy inactive at the maximal soluble
conccntr,.tion (I mM). The results obtained from human hepatoeytes were Clualitatively similar LO those of rat hepatoeytes. hut
staıistieaııy. imp0rlant differenees hetween the two speeies in the amounts of DNA damage and/or unseheduled DNA synthesis were
observed wiıh N-nitrosodimethylamine. f\-nitrosomorpholine, N-nitrosopiperidine. N-nitrosopyrrolidine and N-nitroso-f\.
huıylıırca. On the other hand, 4uantitative differences in the gcnotoxie effeets indueed hy 5 mM-N-nitrosüdimethylaınine in eultures
derived from 20 human donors and from 20 rat were greater than average eoınpounds. These results indieate thaı the rat hepatocyte
DNA repair assay is a valid lI1terspeeies diflen::nccs displayed hy this nitrosamine and hy other N-niıroso model for predicting the
genotoxi" potentıal of N-nitroso compounds in human hepatocytes. 

Anahtar Kelimeler

DNA damage human rat

Kaynakça

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Toplam 1 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Araştırma Makalesi
Yazarlar

Taner Pamukçu

Alexandre Scott

Yayımlanma Tarihi 1 Mart 2002
Yayımlandığı Sayı Yıl 2002Cilt: 49 Sayı: 1

Kaynak Göster

APA Pamukçu, T., & Scott, A. (2002). The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 49(1), 23-29. https://doi.org/10.1501/Vetfak_0000001641
AMA Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. Mart 2002;49(1):23-29. doi:10.1501/Vetfak_0000001641
Chicago Pamukçu, Taner, ve Alexandre Scott. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49, sy. 1 (Mart 2002): 23-29. https://doi.org/10.1501/Vetfak_0000001641.
EndNote Pamukçu T, Scott A (01 Mart 2002) The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49 1 23–29.
IEEE T. Pamukçu ve A. Scott, “The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA”, Ankara Univ Vet Fak Derg, c. 49, sy. 1, ss. 23–29, 2002, doi: 10.1501/Vetfak_0000001641.
ISNAD Pamukçu, Taner - Scott, Alexandre. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 49/1 (Mart 2002), 23-29. https://doi.org/10.1501/Vetfak_0000001641.
JAMA Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. 2002;49:23–29.
MLA Pamukçu, Taner ve Alexandre Scott. “The Eftects of Dinerent N-Nitroso Compounds in Primary Cultures of Human and Rat Hepatocytes on the DNA”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 49, sy. 1, 2002, ss. 23-29, doi:10.1501/Vetfak_0000001641.
Vancouver Pamukçu T, Scott A. The eftects of dinerent N-nitroso compounds in primary cultures of human and rat hepatocytes on the DNA. Ankara Univ Vet Fak Derg. 2002;49(1):23-9.