Ankara Üniversitesi Veteriner Fakültesi Dergisi
Araştırma Makalesi
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Yıl 2019, Cilt: 66 Sayı: 1, 7 - 11, 31.12.2018

Gizem ÇUFAOĞLU Aşkın Nur DERİNÖZ Naim Deniz AYAZ

Öz

Kaynakça

  • 1. Abuladze T, Li M, Menetrez MY, et al. (2008): Bacteriophages reduce experimental contamination of hard surfaces, tomato, spinach, broccoli, and ground beef by Escherichia coli O157:H7. Appl Environ Microbiol, 74, 6230-6238.
  • 2. Aksu H, Özgen Arun Ö, Aydın A, et al (1999): Eschericiha coli O157:H7’nin hayvansal kökenli çeşitli gıda maddelerinde varlığı. Pendik Vet Mikrobiyol Derg, 30, 77-81.
  • 3. Anany H, Chen W, Pelton R, et al. (2011): Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in meat by using phages immobilized on modified cellulose membranes. Appl Environ Microbiol, 77, 6379-6387.
  • 4. Arslan A (2013): Et muayenesi ve Et Ürünleri Teknolojisi. Medipres, Malatya.
  • 5. Ayaz ND, Cufaoglu G, Ormeci E, et al. (2016): Presence of Staphylococcus aureus and Shiga toxigenic Escherichia coli O157:H7 in Raw Meat in Ağrı, Turkey. Int J Enteric Pathog, 4, e36523.
  • 6. Ayaz ND, Gencay YE, Erol I (2014): Prevalence and molecular characterization of sorbitol fermenting and nonfermenting Escherichia coli O157: H7+/H7–isolated from cattle at slaughterhouse and slaughterhouse wastewater. Int J Food Microbiol, 174, 31-38.
  • 7. Balpetek D, Gürbüz Ü (2010): Investigations on the presence of E. coli O157: H7 in some meat products. Eurasian J Vet Sci, 26, 25-31.
  • 8. Bruttin A, Brüssow H (2005): Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy. Antimicrob Agents Chemother, 49, 2874-2878.
  • 9. Brüssow H, Kutter E (2005): Phage ecology. 129-163. In: E Kutter, A Sulakvelizde (Eds), Bacteriophages: biology and applications. CRC Press, Washington DC.
  • 10. Büyükünal SK, Şakar FŞ, Turhan I, et al. (2016): Presence of Salmonella spp., Listeria monocytogenes, Escherichia coli 0157 and Nitrate-Nitrite Residue Levels in Turkish Traditional Fermented Meat Products (Sucuk and Pastirma). Kafkas Univ Vet Fak Derg, 22, 233-236.
  • 11. Chan BK, Abedon ST, Loc-Carrillo C (2013): Phage cocktails and the future of phage therapy. Future Microbiol, 8, 769-783.
  • 12. Erol İ (2007): Gıda Hijyeni ve Mikrobiyolojisi. Pozitif Matbaacılık, Ankara.
  • 13. Erol I, Goncuoglu M, Ayaz ND et al. (2016): Comparison of prevalence and genetic diversity of Escherichia coli O157:H7 in cattle and sheep. J Microbiol Biotechnol Food Sci, 6, 808-812.
  • 14. Gencay YE, Ayaz ND, Copuroglu G, et al. (2016): Biocontrol of Shiga Toxigenic Escherichia coliO157:H7 in Turkish Raw Meatball by Bacteriophage. J Food Saf, 36, 120-131.
  • 15. Guenther S, Huwyler D, Richard S, et al. (2009): Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods. Appl Environ Microbiol, 75, 93-100.
  • 16. Hudson JA, Billington C, Cornelius A, et al. (2013): Use of a bacteriophage to inactivate Escherichia coli O157: H7 on beef. Food Microbiol, 36, 14-21.
  • 17. Kazi M, Annapure US. (2016): Bacteriophage biocontrol of foodborne pathogens. J Food Sci Technol, 53, 1355- 1362.
  • 18. Kudva IT, Jelacic S, Tarr PI, et al. (1999): Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Appl Environ Microbiol, 65, 3767-3773.
  • 19. Leverentz B, Conway WS, Camp MJ, et al. (2003): Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin. Appl Environ Microbiol, 69, 4519-4526.
  • 20. Meng J, Doyle M, Zhao T, et al. (2001): Enterohemorrhagic Escherichia coli O157:H7. 193-213. In: MP Doyle, LR Beuchat, TJ Montville (Eds), Food Microbiology: Fundamentals and Frontiers. ASM press, Washington DC.
  • 21. O'Flaherty S, Ross RP, Coffey A (2009): Bacteriophage and their lysins for elimination of infectious bacteria. FEMS Microbiol Rev, 33, 801-819.
  • 22. O'Flynn G, Ross RP, Fitzgerald GF, et al. (2004): Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol, 70, 3417-3424.
  • 23. Rozema EA, Stephens TP, Bach SJ, et al. (2009): Oral and rectal administration of bacteriophages for control of Escherichia coli O157: H7 in feedlot cattle. J Food Prot, 72, 241-250.
  • 24. Schmidt H, Beutin L, Karch H (1995): Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect immun, 63, 1055-1061.
  • 25. Sharma M, Patel JR, Conway WS, et al. (2009): Effectiveness of bacteriophages in reducing Escherichia coli O157: H7 on fresh-cut cantaloupes and lettuce. J Food Prot, 72, 1481-1485.
  • 26. Tanji Y, Shimada T, Yoichi M, et al. (2004): Toward rational control of Escherichia coli O157: H7 by a phage cocktail. Appl Microbiol Tech, 64, 270-274.
  • 27. Turkish Food Codex Legislation (2000): Et Ürünleri Tebliği. Resmi Gazete, 23960.
  • 28. US Food and Administration (2011): Inventory of effective food contact substance (FCS) notifications. FCN no. 1018. 2011.
  • 29. Viazis S, Akhtar M, Feirtag J, et al. (2011): Reduction of Escherichia coli O157: H7 viability on leafy green vegetables by treatment with a bacteriophage mixture and trans-cinnamaldehyde. Food Microbiol, 28,149-157.
  • 30. Viscardi M, Perugini AG, Auriemma C, et al. (2008): Isolation and characterisation of two novel coliphages with high potential to control antibiotic-resistant pathogenic Escherichia coli (EHEC and EPEC). Int J Antimicrob Agents, 31, 152-157.

An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma

Yıl 2019, Cilt: 66 Sayı: 1, 7 - 11, 31.12.2018

Gizem ÇUFAOĞLU Aşkın Nur DERİNÖZ Naim Deniz AYAZ

Öz

In this study, it was aimed to investigate the biocontrol of Escherichia coli O157:H7 in pastirma (a traditional
Turkish meat product produced and consumed without heat treatment) by bacteriophages. A cocktail was prepared from two precharacterized phages which were isolated from cattle slaughterhouse wastewaters and were found to have lytic activity against E. coli
O157:H7. The phage cocktail at a concentration of 109 pfu/ml was applied to pastirma slices which experimentally contaminated at 6,9
x 103 cfu/ml and incubated for one week at room temperature (22-24ºC). During the incubation period, reduction effects of
bacteriophages on E. coli O157:H7 were investigated on certain times and days. As a result, the phage cocktail was able to reduce the
bacterial count approximately 2 log cfu/g in the bacteriophage applied group, therefore the number of E. coli O157:H7 decreased and
remained below the detection limit (< 10 cfu/g) during the experiment. The bacteriophage cocktail consisting of phage M8AEC16 and
phage M12BEC16 has been found to be effectively usable for biocontrol of E. coli O157:H7 in pastirma.  

Anahtar Kelimeler

Bacteriophage, biocontrol, E. coli O157:H7, pastirma

Kaynakça

  • 1. Abuladze T, Li M, Menetrez MY, et al. (2008): Bacteriophages reduce experimental contamination of hard surfaces, tomato, spinach, broccoli, and ground beef by Escherichia coli O157:H7. Appl Environ Microbiol, 74, 6230-6238.
  • 2. Aksu H, Özgen Arun Ö, Aydın A, et al (1999): Eschericiha coli O157:H7’nin hayvansal kökenli çeşitli gıda maddelerinde varlığı. Pendik Vet Mikrobiyol Derg, 30, 77-81.
  • 3. Anany H, Chen W, Pelton R, et al. (2011): Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in meat by using phages immobilized on modified cellulose membranes. Appl Environ Microbiol, 77, 6379-6387.
  • 4. Arslan A (2013): Et muayenesi ve Et Ürünleri Teknolojisi. Medipres, Malatya.
  • 5. Ayaz ND, Cufaoglu G, Ormeci E, et al. (2016): Presence of Staphylococcus aureus and Shiga toxigenic Escherichia coli O157:H7 in Raw Meat in Ağrı, Turkey. Int J Enteric Pathog, 4, e36523.
  • 6. Ayaz ND, Gencay YE, Erol I (2014): Prevalence and molecular characterization of sorbitol fermenting and nonfermenting Escherichia coli O157: H7+/H7–isolated from cattle at slaughterhouse and slaughterhouse wastewater. Int J Food Microbiol, 174, 31-38.
  • 7. Balpetek D, Gürbüz Ü (2010): Investigations on the presence of E. coli O157: H7 in some meat products. Eurasian J Vet Sci, 26, 25-31.
  • 8. Bruttin A, Brüssow H (2005): Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy. Antimicrob Agents Chemother, 49, 2874-2878.
  • 9. Brüssow H, Kutter E (2005): Phage ecology. 129-163. In: E Kutter, A Sulakvelizde (Eds), Bacteriophages: biology and applications. CRC Press, Washington DC.
  • 10. Büyükünal SK, Şakar FŞ, Turhan I, et al. (2016): Presence of Salmonella spp., Listeria monocytogenes, Escherichia coli 0157 and Nitrate-Nitrite Residue Levels in Turkish Traditional Fermented Meat Products (Sucuk and Pastirma). Kafkas Univ Vet Fak Derg, 22, 233-236.
  • 11. Chan BK, Abedon ST, Loc-Carrillo C (2013): Phage cocktails and the future of phage therapy. Future Microbiol, 8, 769-783.
  • 12. Erol İ (2007): Gıda Hijyeni ve Mikrobiyolojisi. Pozitif Matbaacılık, Ankara.
  • 13. Erol I, Goncuoglu M, Ayaz ND et al. (2016): Comparison of prevalence and genetic diversity of Escherichia coli O157:H7 in cattle and sheep. J Microbiol Biotechnol Food Sci, 6, 808-812.
  • 14. Gencay YE, Ayaz ND, Copuroglu G, et al. (2016): Biocontrol of Shiga Toxigenic Escherichia coliO157:H7 in Turkish Raw Meatball by Bacteriophage. J Food Saf, 36, 120-131.
  • 15. Guenther S, Huwyler D, Richard S, et al. (2009): Virulent bacteriophage for efficient biocontrol of Listeria monocytogenes in ready-to-eat foods. Appl Environ Microbiol, 75, 93-100.
  • 16. Hudson JA, Billington C, Cornelius A, et al. (2013): Use of a bacteriophage to inactivate Escherichia coli O157: H7 on beef. Food Microbiol, 36, 14-21.
  • 17. Kazi M, Annapure US. (2016): Bacteriophage biocontrol of foodborne pathogens. J Food Sci Technol, 53, 1355- 1362.
  • 18. Kudva IT, Jelacic S, Tarr PI, et al. (1999): Biocontrol of Escherichia coli O157 with O157-specific bacteriophages. Appl Environ Microbiol, 65, 3767-3773.
  • 19. Leverentz B, Conway WS, Camp MJ, et al. (2003): Biocontrol of Listeria monocytogenes on fresh-cut produce by treatment with lytic bacteriophages and a bacteriocin. Appl Environ Microbiol, 69, 4519-4526.
  • 20. Meng J, Doyle M, Zhao T, et al. (2001): Enterohemorrhagic Escherichia coli O157:H7. 193-213. In: MP Doyle, LR Beuchat, TJ Montville (Eds), Food Microbiology: Fundamentals and Frontiers. ASM press, Washington DC.
  • 21. O'Flaherty S, Ross RP, Coffey A (2009): Bacteriophage and their lysins for elimination of infectious bacteria. FEMS Microbiol Rev, 33, 801-819.
  • 22. O'Flynn G, Ross RP, Fitzgerald GF, et al. (2004): Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol, 70, 3417-3424.
  • 23. Rozema EA, Stephens TP, Bach SJ, et al. (2009): Oral and rectal administration of bacteriophages for control of Escherichia coli O157: H7 in feedlot cattle. J Food Prot, 72, 241-250.
  • 24. Schmidt H, Beutin L, Karch H (1995): Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157: H7 strain EDL 933. Infect immun, 63, 1055-1061.
  • 25. Sharma M, Patel JR, Conway WS, et al. (2009): Effectiveness of bacteriophages in reducing Escherichia coli O157: H7 on fresh-cut cantaloupes and lettuce. J Food Prot, 72, 1481-1485.
  • 26. Tanji Y, Shimada T, Yoichi M, et al. (2004): Toward rational control of Escherichia coli O157: H7 by a phage cocktail. Appl Microbiol Tech, 64, 270-274.
  • 27. Turkish Food Codex Legislation (2000): Et Ürünleri Tebliği. Resmi Gazete, 23960.
  • 28. US Food and Administration (2011): Inventory of effective food contact substance (FCS) notifications. FCN no. 1018. 2011.
  • 29. Viazis S, Akhtar M, Feirtag J, et al. (2011): Reduction of Escherichia coli O157: H7 viability on leafy green vegetables by treatment with a bacteriophage mixture and trans-cinnamaldehyde. Food Microbiol, 28,149-157.
  • 30. Viscardi M, Perugini AG, Auriemma C, et al. (2008): Isolation and characterisation of two novel coliphages with high potential to control antibiotic-resistant pathogenic Escherichia coli (EHEC and EPEC). Int J Antimicrob Agents, 31, 152-157.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Gizem ÇUFAOĞLU

Aşkın Nur DERİNÖZ

Naim Deniz AYAZ

Yayımlanma Tarihi 31 Aralık 2018
Yayınlandığı Sayı Yıl 2019Cilt: 66 Sayı: 1

Kaynak Göster

Bibtex @araştırma makalesi { auvfd635761, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2019}, volume = {66}, number = {1}, pages = {7 - 11}, title = {An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma}, key = {cite}, author = {Çufaoğlu, Gizem and Derinöz, Aşkın Nur and Ayaz, Naim Deniz} }
APA Çufaoğlu, G. , Derinöz, A. N. & Ayaz, N. D. (2019). An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 66 (1) , 7-11 . Retrieved from http://vetjournal.ankara.edu.tr/tr/pub/issue/49606/635761
MLA Çufaoğlu, G. , Derinöz, A. N. , Ayaz, N. D. "An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 7-11 <http://vetjournal.ankara.edu.tr/tr/pub/issue/49606/635761>
Chicago Çufaoğlu, G. , Derinöz, A. N. , Ayaz, N. D. "An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 7-11
RIS TY - JOUR T1 - An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma AU - GizemÇufaoğlu, Aşkın NurDerinöz, Naim DenizAyaz Y1 - 2019 PY - 2019 N1 - DO - T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 7 EP - 11 VL - 66 IS - 1 SN - 1300-0861-1308-2817 M3 - UR - Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma %A Gizem Çufaoğlu , Aşkın Nur Derinöz , Naim Deniz Ayaz %T An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma %D 2019 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 66 %N 1 %R %U
ISNAD Çufaoğlu, Gizem , Derinöz, Aşkın Nur , Ayaz, Naim Deniz . "An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 / 1 (Aralık 2018): 7-11 .
AMA Çufaoğlu G. , Derinöz A. N. , Ayaz N. D. An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Univ Vet Fak Derg. 2019; 66(1): 7-11.
Vancouver Çufaoğlu G. , Derinöz A. N. , Ayaz N. D. An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2019; 66(1): 7-11.
IEEE G. Çufaoğlu , A. N. Derinöz ve N. D. Ayaz , "An investigation on biocontrol of Escherichia coli O157:H7 by a bacteriophage cocktail in pastirma", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 66, sayı. 1, ss. 7-11, Ara. 2018