Araştırma Makalesi
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Yıl 2019, Cilt: 66 Sayı: 1, 83 - 87, 31.12.2018

Öz

Kaynakça

  • 1. Alçay S, Üstüner B, Nur Z (2016): Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability. Small Ruminant Research, 136, 59-64.
  • 2. Ali J, Shelton J (1993): Successful vitrification of day-6 sheep embryos. Journal of J Reprod Fert, 99, 65-70. 3. Bavister BD (1998): Role of oviductal secretions in embryonic growth in vivo and in vitro. Theriogenology, 29, 143-154.
  • 4. Birler S, Pabuccuoğlu S, Alkan S, et al. (2001): Effects of different maturation and culture media on IVF of sheep oocytes. Pakistan Vet J, 100-6 ,102.
  • 5. De Paz P, Sanchez A, Fernandez J, et al. (1994): Sheep embryo cryopreservation by vitrification and conventional freezing. Theriogenology, 42, 327-338.
  • 6. Donnay I, Van Langendonckt A, Auquier P, et al. (1997): Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology, 47, 1549- 1561.
  • 7. Gandolfi F, Moor R (1987): Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J Reprod Fert, 81,23-28.
  • 8. Garcia-Garcia R, Gonzalez-Bulnes A, Dominguez V, et al. (2006): Survival of frozen-thawed sheep embryos cryopreserved at cleavage stages. Cryobiology, 52, 108- 113.
  • 9. Gupta MK, Lee HT (2010): Cryopreservation of oocytes and embryos by vitrification. Korean J Reprod Med, 37, 267-291.
  • 10. Heidari B, Shirazi A, Naderi M, et al. (2013): Effect of various co-culture systems on embryo development in ovine. Czech J Anim Sci, 58, 443-452.
  • 11. Jang H, Jung Y, Cheong H, et al. (2008): Effects of cell status of bovine oviduct epithelial cell (BOEC) on the development of bovine IVM/IVF embryos and gene expression in the BOEC used or not used for the embryo culture. Asian-Aust J Anim Sci, 21, 980-987.
  • 12. Ledda S, Bogliolo L, Leoni G, et al. (1995): Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. Reprod Nutr Dev, 35, 629-637.
  • 13. Manokaran S, Veerapandian C, Balasubramanian S (2012): In vitro fertilization of sheep oocytes matured in two different media. Indian J Small Ruminants, 18, 44-46.
  • 14. Martinez A, Matkovic M (1998): Cryopreservation of ovine embryos: slow freezing and vitrification. Theriogenology, 49, 1039-1049.
  • 15. Martínez A, Valcárcel A, Furnus C, et al. (2006): Cryopreservation of in vitro-produced ovine embryos. Small Ruminant Research, 63, 288-296.
  • 16. Nedambale T, Dinnyes A, Groen W, et al. (2004): Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology, 62, 437-449.
  • 17. Niemann H (1991): Cryopreservation of ova and embryos from livestock: current status and research needs. Theriogenology, 35, 109-124.
  • 18. Paramio MT, Izquierdo D (2014): Current status of in vitro embryo production in sheep and goats. Reprod Dom Anim, 49, 37- 48.
  • 19. Pereira R, Marques C (2008): Animal oocyte and embryo cryopreservation. Cell and Tissue Banking, 9, 267-277.
  • 20. Riha J, Machatkova M, Pavlok A (2002): Viability of fresh and frozen transferred IVP bovine embryos. Czech J Anim Sci, 47, 261-267.
  • 21. Sakkas D, Trounson A, Kola I (1989): In vivo cleavage rates and viability obtained for early cleavage mouse embryos in co-culture with oviduct cells. Reprod Fer Dev, 1, 127-136.
  • 22. Schiewe M, Rall W, Stuart L, et al. (1991): Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos. Theriogenology, 36, 279-293.
  • 23. Shirazi A, Motaghi E (2013): The in vitro fertilization of ovine oocytes in the presence of oviductal cells and its effect on the expression of zygote arrest 1 (zar1) and subsequent embryonic development. J Reprod Infert, 14, 8-16
  • 24. Songsasen N, Buckrell B, Plante C, et al. (1995): In vitro and in vivo survival of cryopreserved sheep embryos. Cryobiology, 32, 78-91.
  • 25. Tominaga K (2004): Cryopreservation and sexing of in vivo-and in vitro-produced bovine embryos for their practical use. Journal of Reproduction and Development, 50, 29-38.
  • 26. Vajta G (2000): Vitrification of the oocytes and embryos of domestic animals. Anim Reprod, 60, 357-364.
  • 27. Van Wagtendonk-de Leeuw A, Mullaart E, De Roos A, et al. (2000): Effects of different reproduction techniques: AI, MOET or IVP, on health and welfare of bovine offspring. Theriogenology, 53, 575-597.
  • 28. Villamil PR, Lozano D, Oviedo J, et al. (2012): Developmental rates of in vivo and in vitro produced bovine embryos cryopreserved in ethylene glycol based solutions by slow freezing or solid surface vitrification. Anim Reprod, 9, 86-92.
  • 29. Yadav P, Saini A, Kumar A, et al. (1998): Effect of oviductal cell co-culture on cleavage and development of goat IVF embryos. Anim Reprod, 51, 301-306.
  • 30. Yang N, Lu K, Gordon I, et al. (1992): Vitrification of bovine blastocysts produced in vitro. Theriogenology, 37, 326 (abstract).
  • 31. Youngs CR (2011): Cryopreservation of preimplantation embryos of cattle, sheep, and goats. J Vis Exp, 54, 2764

Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro

Yıl 2019, Cilt: 66 Sayı: 1, 83 - 87, 31.12.2018

Öz

The effects of two vitrification protocols on the survival of sheep blastocysts were examined in embryos produced
in vitro with sheep oviduct epithelial cells co-culture (CC) or without co-culture (C). Oocytes collected from slaughtered ewes were
matured for 24 h, fertilized with fresh ram semen for 20 h and cultured in SOF medium for up to 9 days in vitro. For vitrification,
blastocyst stage embryos were assigned to two equilibration groups (20% ethylen glycol (EG) or 10% glycerol (G) for the first
equilibration), and as the second equilibration they were kept in 20% ethylen glycol plus 10% glycerol for 5 minutes. After 30 sec in
vitrification solution (25% ethylen glycol + 25% glycerol), they were immediately immersed into liquid nitrogen. After thawing
procedure, embryos were transferred into 0.25 M sucrose for 5 min, washed in Hepes buffered synthetic oviduct fluid (HSOF) and
cultured in SOF medium for 24 h. Cleavage rates were 75.2% in C and 74.2% in CC groups, and blastocyst rates were 14.4% in C and
17.1% in CC groups. After in vitro culture of vitrified-thawed blastocysts, survival rates were 62.1, 38.4, 30.2, and 39.3% in C-EG,
CC-EG, C-G and CC-G groups, respectively. This study shows that vitrification of sheep embryos using ethylene glycol instead of
glycerol as a first equilibration cryoprotectant could give reasonable survival rates and that co-culture of embryos with sheep oviduct
epithelial cell has no beneficial effect on vitrification of embryos.
  

Kaynakça

  • 1. Alçay S, Üstüner B, Nur Z (2016): Effects of low molecular weight cryoprotectants on the post-thaw ram sperm quality and fertilizing ability. Small Ruminant Research, 136, 59-64.
  • 2. Ali J, Shelton J (1993): Successful vitrification of day-6 sheep embryos. Journal of J Reprod Fert, 99, 65-70. 3. Bavister BD (1998): Role of oviductal secretions in embryonic growth in vivo and in vitro. Theriogenology, 29, 143-154.
  • 4. Birler S, Pabuccuoğlu S, Alkan S, et al. (2001): Effects of different maturation and culture media on IVF of sheep oocytes. Pakistan Vet J, 100-6 ,102.
  • 5. De Paz P, Sanchez A, Fernandez J, et al. (1994): Sheep embryo cryopreservation by vitrification and conventional freezing. Theriogenology, 42, 327-338.
  • 6. Donnay I, Van Langendonckt A, Auquier P, et al. (1997): Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology, 47, 1549- 1561.
  • 7. Gandolfi F, Moor R (1987): Stimulation of early embryonic development in the sheep by co-culture with oviduct epithelial cells. J Reprod Fert, 81,23-28.
  • 8. Garcia-Garcia R, Gonzalez-Bulnes A, Dominguez V, et al. (2006): Survival of frozen-thawed sheep embryos cryopreserved at cleavage stages. Cryobiology, 52, 108- 113.
  • 9. Gupta MK, Lee HT (2010): Cryopreservation of oocytes and embryos by vitrification. Korean J Reprod Med, 37, 267-291.
  • 10. Heidari B, Shirazi A, Naderi M, et al. (2013): Effect of various co-culture systems on embryo development in ovine. Czech J Anim Sci, 58, 443-452.
  • 11. Jang H, Jung Y, Cheong H, et al. (2008): Effects of cell status of bovine oviduct epithelial cell (BOEC) on the development of bovine IVM/IVF embryos and gene expression in the BOEC used or not used for the embryo culture. Asian-Aust J Anim Sci, 21, 980-987.
  • 12. Ledda S, Bogliolo L, Leoni G, et al. (1995): Two culture systems showing a biphasic effect on ovine embryo development from the 1-2 cell stage to hatched blastocysts. Reprod Nutr Dev, 35, 629-637.
  • 13. Manokaran S, Veerapandian C, Balasubramanian S (2012): In vitro fertilization of sheep oocytes matured in two different media. Indian J Small Ruminants, 18, 44-46.
  • 14. Martinez A, Matkovic M (1998): Cryopreservation of ovine embryos: slow freezing and vitrification. Theriogenology, 49, 1039-1049.
  • 15. Martínez A, Valcárcel A, Furnus C, et al. (2006): Cryopreservation of in vitro-produced ovine embryos. Small Ruminant Research, 63, 288-296.
  • 16. Nedambale T, Dinnyes A, Groen W, et al. (2004): Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology, 62, 437-449.
  • 17. Niemann H (1991): Cryopreservation of ova and embryos from livestock: current status and research needs. Theriogenology, 35, 109-124.
  • 18. Paramio MT, Izquierdo D (2014): Current status of in vitro embryo production in sheep and goats. Reprod Dom Anim, 49, 37- 48.
  • 19. Pereira R, Marques C (2008): Animal oocyte and embryo cryopreservation. Cell and Tissue Banking, 9, 267-277.
  • 20. Riha J, Machatkova M, Pavlok A (2002): Viability of fresh and frozen transferred IVP bovine embryos. Czech J Anim Sci, 47, 261-267.
  • 21. Sakkas D, Trounson A, Kola I (1989): In vivo cleavage rates and viability obtained for early cleavage mouse embryos in co-culture with oviduct cells. Reprod Fer Dev, 1, 127-136.
  • 22. Schiewe M, Rall W, Stuart L, et al. (1991): Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos. Theriogenology, 36, 279-293.
  • 23. Shirazi A, Motaghi E (2013): The in vitro fertilization of ovine oocytes in the presence of oviductal cells and its effect on the expression of zygote arrest 1 (zar1) and subsequent embryonic development. J Reprod Infert, 14, 8-16
  • 24. Songsasen N, Buckrell B, Plante C, et al. (1995): In vitro and in vivo survival of cryopreserved sheep embryos. Cryobiology, 32, 78-91.
  • 25. Tominaga K (2004): Cryopreservation and sexing of in vivo-and in vitro-produced bovine embryos for their practical use. Journal of Reproduction and Development, 50, 29-38.
  • 26. Vajta G (2000): Vitrification of the oocytes and embryos of domestic animals. Anim Reprod, 60, 357-364.
  • 27. Van Wagtendonk-de Leeuw A, Mullaart E, De Roos A, et al. (2000): Effects of different reproduction techniques: AI, MOET or IVP, on health and welfare of bovine offspring. Theriogenology, 53, 575-597.
  • 28. Villamil PR, Lozano D, Oviedo J, et al. (2012): Developmental rates of in vivo and in vitro produced bovine embryos cryopreserved in ethylene glycol based solutions by slow freezing or solid surface vitrification. Anim Reprod, 9, 86-92.
  • 29. Yadav P, Saini A, Kumar A, et al. (1998): Effect of oviductal cell co-culture on cleavage and development of goat IVF embryos. Anim Reprod, 51, 301-306.
  • 30. Yang N, Lu K, Gordon I, et al. (1992): Vitrification of bovine blastocysts produced in vitro. Theriogenology, 37, 326 (abstract).
  • 31. Youngs CR (2011): Cryopreservation of preimplantation embryos of cattle, sheep, and goats. J Vis Exp, 54, 2764

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Hatem ATALLA


Mithat EVECEN


Serhat PABUCCUOĞLU


Sema BİRLER

Yayımlanma Tarihi 31 Aralık 2018
Yayınlandığı Sayı Yıl 2019Cilt: 66 Sayı: 1

Kaynak Göster

Bibtex @araştırma makalesi { auvfd636019, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2019}, volume = {66}, number = {1}, pages = {83 - 87}, title = {Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro}, key = {cite}, author = {Atalla, Hatem and Evecen, Mithat and Pabuccuoğlu, Serhat and Birler, Sema} }
APA Atalla, H. , Evecen, M. , Pabuccuoğlu, S. & Birler, S. (2019). Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 66 (1) , 83-87 . Retrieved from http://vetjournal.ankara.edu.tr/tr/pub/issue/49606/636019
MLA Atalla, H. , Evecen, M. , Pabuccuoğlu, S. , Birler, S. "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 83-87 <http://vetjournal.ankara.edu.tr/tr/pub/issue/49606/636019>
Chicago Atalla, H. , Evecen, M. , Pabuccuoğlu, S. , Birler, S. "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 83-87
RIS TY - JOUR T1 - Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro AU - HatemAtalla, MithatEvecen, SerhatPabuccuoğlu, SemaBirler Y1 - 2019 PY - 2019 N1 - DO - T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 83 EP - 87 VL - 66 IS - 1 SN - 1300-0861-1308-2817 M3 - UR - Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro %A Hatem Atalla , Mithat Evecen , Serhat Pabuccuoğlu , Sema Birler %T Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro %D 2019 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 66 %N 1 %R %U
ISNAD Atalla, Hatem , Evecen, Mithat , Pabuccuoğlu, Serhat , Birler, Sema . "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 / 1 (Aralık 2018): 83-87 .
AMA Atalla H. , Evecen M. , Pabuccuoğlu S. , Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Univ Vet Fak Derg. 2019; 66(1): 83-87.
Vancouver Atalla H. , Evecen M. , Pabuccuoğlu S. , Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2019; 66(1): 83-87.
IEEE H. Atalla , M. Evecen , S. Pabuccuoğlu ve S. Birler , "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 66, sayı. 1, ss. 83-87, Ara. 2018