The effects of two vitrification protocols on the survival of sheep blastocysts were examined in embryos produced
in vitro with sheep oviduct epithelial cells co-culture (CC) or without co-culture (C). Oocytes collected from slaughtered ewes were
matured for 24 h, fertilized with fresh ram semen for 20 h and cultured in SOF medium for up to 9 days in vitro. For vitrification,
blastocyst stage embryos were assigned to two equilibration groups (20% ethylen glycol (EG) or 10% glycerol (G) for the first
equilibration), and as the second equilibration they were kept in 20% ethylen glycol plus 10% glycerol for 5 minutes. After 30 sec in
vitrification solution (25% ethylen glycol + 25% glycerol), they were immediately immersed into liquid nitrogen. After thawing
procedure, embryos were transferred into 0.25 M sucrose for 5 min, washed in Hepes buffered synthetic oviduct fluid (HSOF) and
cultured in SOF medium for 24 h. Cleavage rates were 75.2% in C and 74.2% in CC groups, and blastocyst rates were 14.4% in C and
17.1% in CC groups. After in vitro culture of vitrified-thawed blastocysts, survival rates were 62.1, 38.4, 30.2, and 39.3% in C-EG,
CC-EG, C-G and CC-G groups, respectively. This study shows that vitrification of sheep embryos using ethylene glycol instead of
glycerol as a first equilibration cryoprotectant could give reasonable survival rates and that co-culture of embryos with sheep oviduct
epithelial cell has no beneficial effect on vitrification of embryos.
Co-culture, equilibration medium, in vitro fertilization, sheep vitrification
Birincil Dil | İngilizce |
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Bölüm | Araştırma Makalesi |
Yazarlar |
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Yayımlanma Tarihi | 31 Aralık 2018 |
Yayınlandığı Sayı | Yıl 2019Cilt: 66 Sayı: 1 |
Bibtex | @araştırma makalesi { auvfd636019, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2019}, volume = {66}, number = {1}, pages = {83 - 87}, title = {Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro}, key = {cite}, author = {Atalla, Hatem and Evecen, Mithat and Pabuccuoğlu, Serhat and Birler, Sema} } |
APA | Atalla, H. , Evecen, M. , Pabuccuoğlu, S. & Birler, S. (2019). Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 66 (1) , 83-87 . Retrieved from http://vetjournal.ankara.edu.tr/tr/pub/issue/49606/636019 |
MLA | Atalla, H. , Evecen, M. , Pabuccuoğlu, S. , Birler, S. "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 83-87 <http://vetjournal.ankara.edu.tr/tr/pub/issue/49606/636019> |
Chicago | Atalla, H. , Evecen, M. , Pabuccuoğlu, S. , Birler, S. "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 (2019 ): 83-87 |
RIS | TY - JOUR T1 - Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro AU - HatemAtalla, MithatEvecen, SerhatPabuccuoğlu, SemaBirler Y1 - 2019 PY - 2019 N1 - DO - T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 83 EP - 87 VL - 66 IS - 1 SN - 1300-0861-1308-2817 M3 - UR - Y2 - 2023 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro %A Hatem Atalla , Mithat Evecen , Serhat Pabuccuoğlu , Sema Birler %T Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro %D 2019 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 66 %N 1 %R %U |
ISNAD | Atalla, Hatem , Evecen, Mithat , Pabuccuoğlu, Serhat , Birler, Sema . "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro". Ankara Üniversitesi Veteriner Fakültesi Dergisi 66 / 1 (Aralık 2018): 83-87 . |
AMA | Atalla H. , Evecen M. , Pabuccuoğlu S. , Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Univ Vet Fak Derg. 2019; 66(1): 83-87. |
Vancouver | Atalla H. , Evecen M. , Pabuccuoğlu S. , Birler S. Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2019; 66(1): 83-87. |
IEEE | H. Atalla , M. Evecen , S. Pabuccuoğlu ve S. Birler , "Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 66, sayı. 1, ss. 83-87, Ara. 2018 |