Yıl 2021, Cilt 68 , Sayı 2, Sayfalar 97 - 105 2021-03-31

Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey

Bekir OĞUZ [1] , Mustafa Serdar DEĞER [2] , Sena KOŞAL [3]


This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.
18S rRNA, Bovine minced meats, PCR, Sarcocystis spp.
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Birincil Dil en
Konular Veteriner Hekimlik
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0001-8720-3940
Yazar: Bekir OĞUZ (Sorumlu Yazar)
Kurum: Van Yüzüncü Yıl University, Faculty of Veterinary Medicine, Department of Parasitology, Van
Ülke: Turkey


Orcid: 0000-0001-9651-6957
Yazar: Mustafa Serdar DEĞER
Kurum: Van Yüzüncü Yıl University, Faculty of Veterinary Medicine, Department of Parasitology, Van
Ülke: Turkey


Orcid: 0000-0002-2048-6947
Yazar: Sena KOŞAL
Kurum: Van Yüzüncü Yıl University, Faculty of Veterinary Medicine, Department of Parasitology, Van
Ülke: Turkey


Destekleyen Kurum Van Yüzüncü Yıl University
Proje Numarası YYUBAP/TSA-2018-7456
Tarihler

Yayımlanma Tarihi : 31 Mart 2021

Bibtex @araştırma makalesi { auvfd671606, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2021}, volume = {68}, pages = {97 - 105}, doi = {10.33988/auvfd.671606}, title = {Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey}, key = {cite}, author = {Oğuz, Bekir and Değer, Mustafa Serdar and Koşal, Sena} }
APA Oğuz, B , Değer, M , Koşal, S . (2021). Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 68 (2) , 97-105 . DOI: 10.33988/auvfd.671606
MLA Oğuz, B , Değer, M , Koşal, S . "Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 (2021 ): 97-105 <http://vetjournal.ankara.edu.tr/tr/pub/issue/60673/671606>
Chicago Oğuz, B , Değer, M , Koşal, S . "Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 (2021 ): 97-105
RIS TY - JOUR T1 - Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey AU - Bekir Oğuz , Mustafa Serdar Değer , Sena Koşal Y1 - 2021 PY - 2021 N1 - doi: 10.33988/auvfd.671606 DO - 10.33988/auvfd.671606 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 97 EP - 105 VL - 68 IS - 2 SN - -1308-2817 M3 - doi: 10.33988/auvfd.671606 UR - https://doi.org/10.33988/auvfd.671606 Y2 - 2020 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey %A Bekir Oğuz , Mustafa Serdar Değer , Sena Koşal %T Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey %D 2021 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 68 %N 2 %R doi: 10.33988/auvfd.671606 %U 10.33988/auvfd.671606
ISNAD Oğuz, Bekir , Değer, Mustafa Serdar , Koşal, Sena . "Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 / 2 (Mart 2021): 97-105 . https://doi.org/10.33988/auvfd.671606
AMA Oğuz B , Değer M , Koşal S . Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey. Ankara Univ Vet Fak Derg. 2021; 68(2): 97-105.
Vancouver Oğuz B , Değer M , Koşal S . Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 97-105.
IEEE B. Oğuz , M. Değer ve S. Koşal , "Molecular identification using 18S ribosomal RNA of Sarcocystis spp. in bovine minced meat in Van Province, Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 68, sayı. 2, ss. 97-105, Mar. 2021, doi:10.33988/auvfd.671606