Yıl 2021, Cilt 68 , Sayı 2, Sayfalar 167 - 172 2021-03-31

The aim of this study was to determine an infectious bronchitis (IB) infection, caused by an Israel variant 2 (IS/1494/06)-like IBV, in a layer chicken flock regularly vaccinated with vaccines containing IBV H120 and 4/91 strains. Mild respiratory symptoms, drop in egg production and soft-shelled eggs and eventually death were observed in a layer chicken flock. Clinical samples from four diseased chickens were examined for the detection and genotyping of IBV by virus isolation, a commercial real time reverse transcription polymerase chain reaction (rRT-PCR) and nucleotide sequencing. Both Israel variant 2 (IS-Var2) and 793/B serotypes were detected from samples by rRT-PCR, but sequencing results of a 345 bp part of S1 gene revealed that our IBV isolate, HFT-IBV, was IS/1494/06 (IS-Var2)-like with the 97.7% genetic similarity. These results suggested that immunity against vaccination with a combination of different genotypes (H120 and 4/91) could not be protective for IS-Var2 IBV field infection. In addition, identification of genotypes from the clinical samples, such as swabs and organ samples by commercial rRT-PCR assays failed to find correct IBV genotype responsible for the IB infection, whereas analysis of nucleotide sequencing of S1 gene of IBV as a gold standard test undoubtedly detected causative genotype of the infection. Also, the findings indicated that there is an urgent need for consider genotype- or protectotype-match vaccination strategies in the field to prevent vaccine- and IB-dependent economic losses of the poultry sector and logically protect chickens from IBV infection.
genotype, Infectious Bronchitis Virus, Israel variant 2, Layer chicken, vaccination.
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Birincil Dil en
Konular Veteriner Hekimlik
Bölüm Araştırma Makalesi
Yazarlar

Orcid: 0000-0003-4978-9578
Yazar: Hasan ÖNGÖR (Sorumlu Yazar)
Kurum: Fırat University, Faculty of Veterinary Medicine, Department of Microbiology, Elazığ
Ülke: Turkey


Orcid: 0000-0001-5567-991X
Yazar: Necati TİMURKAAN
Kurum: Fırat University, Faculty of Veterinary Medicine, Department of Pathology, Elazığ
Ülke: Turkey


Orcid: 0000-0003-1928-7630
Yazar: Fethiye ÇÖVEN
Kurum: Bornova Veterinary Control Institute, İzmir
Ülke: Turkey


Orcid: 0000-0002-4907-6159
Yazar: Burak KARABULUT
Kurum: Fırat University, Faculty of Veterinary Medicine, Department of Pathology, Elazığ
Ülke: Turkey


Orcid: 0000-0002-8407-5792
Yazar: Hatice ERÖKSÜZ
Kurum: Fırat University, Faculty of Veterinary Medicine, Department of Pathology, Elazığ
Ülke: Turkey


Orcid: 0000-0001-6347-1684
Yazar: Burhan ÇETİNKAYA
Kurum: Fırat University, Faculty of Veterinary Medicine, Department of Microbiology, Elazığ
Ülke: Turkey


Orcid: 0000-0001-6045-8644
Yazar: Tayfun CARLI
Kurum: Uludağ University, Faculty of Veterinary Medicine, Department of Microbiology, Bursa
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 31 Mart 2021

Bibtex @araştırma makalesi { auvfd756970, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2021}, volume = {68}, pages = {167 - 172}, doi = {10.33988/auvfd.756970}, title = {Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock}, key = {cite}, author = {Öngör, Hasan and Timurkaan, Necati and Çöven, Fethiye and Karabulut, Burak and Eröksüz, Hatice and Çetinkaya, Burhan and Carlı, Tayfun} }
APA Öngör, H , Timurkaan, N , Çöven, F , Karabulut, B , Eröksüz, H , Çetinkaya, B , Carlı, T . (2021). Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 68 (2) , 167-172 . DOI: 10.33988/auvfd.756970
MLA Öngör, H , Timurkaan, N , Çöven, F , Karabulut, B , Eröksüz, H , Çetinkaya, B , Carlı, T . "Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 (2021 ): 167-172 <http://vetjournal.ankara.edu.tr/tr/pub/issue/60673/756970>
Chicago Öngör, H , Timurkaan, N , Çöven, F , Karabulut, B , Eröksüz, H , Çetinkaya, B , Carlı, T . "Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 (2021 ): 167-172
RIS TY - JOUR T1 - Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock AU - Hasan Öngör , Necati Timurkaan , Fethiye Çöven , Burak Karabulut , Hatice Eröksüz , Burhan Çetinkaya , Tayfun Carlı Y1 - 2021 PY - 2021 N1 - doi: 10.33988/auvfd.756970 DO - 10.33988/auvfd.756970 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 167 EP - 172 VL - 68 IS - 2 SN - -1308-2817 M3 - doi: 10.33988/auvfd.756970 UR - https://doi.org/10.33988/auvfd.756970 Y2 - 2020 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock %A Hasan Öngör , Necati Timurkaan , Fethiye Çöven , Burak Karabulut , Hatice Eröksüz , Burhan Çetinkaya , Tayfun Carlı %T Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock %D 2021 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 68 %N 2 %R doi: 10.33988/auvfd.756970 %U 10.33988/auvfd.756970
ISNAD Öngör, Hasan , Timurkaan, Necati , Çöven, Fethiye , Karabulut, Burak , Eröksüz, Hatice , Çetinkaya, Burhan , Carlı, Tayfun . "Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock". Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 / 2 (Mart 2021): 167-172 . https://doi.org/10.33988/auvfd.756970
AMA Öngör H , Timurkaan N , Çöven F , Karabulut B , Eröksüz H , Çetinkaya B , Carlı T . Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Univ Vet Fak Derg. 2021; 68(2): 167-172.
Vancouver Öngör H , Timurkaan N , Çöven F , Karabulut B , Eröksüz H , Çetinkaya B , Carlı T . Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2021; 68(2): 167-172.
IEEE H. Öngör , N. Timurkaan , F. Çöven , B. Karabulut , H. Eröksüz , B. Çetinkaya ve T. Carlı , "Detection of Israel variant 2 (IS/1494/06) genotype of Infectious Bronchitis Virus in a layer chicken flock", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 68, sayı. 2, ss. 167-172, Mar. 2021, doi:10.33988/auvfd.756970