The main object of the study is to demonstrate that Bruce-ladder multiplex PCR, which is capable of identify all Brucella species including vaccinal strains, is a safe and practical method that is alternative to bacteriological culture methods. Brucellosis is a zoonosis of great socio-economic importance which is endemic in Turkey. In three-year period from 2009 to 2011, a total of 281 Brucella spp. isolates from cattle, sheep and goats were identified and characterized by both conventional biotyping procedures and the PCR based method called Bruce-ladder. Species identification from both phenotypic testing and Bruce-ladder was concordant for all isolates tested. The results showed preferred host pattern in B.abortus isolates. In terms of species identity, it was found that 94% of the isolations derived from cattle were B.abortus and were exclusively biotype 3. B.melitensis biotype 3 and biotype 1 were the prevelant biotypes in sheep and goats. Two isolates from sheep were identified as B.melitensis Rev.1 vaccine strain. Surprisingly, one of the goat isolates was identified as B.abortus S19 vaccine strain. Other interesting observations were the isolation of penicilin-sensitive non vaccine strains of B.abortus and B.melitensis as well as an isolation of an atypical B.abortus biotype 3 strain on the basis of growth inhibition in the presence of basic fuchsin dye. At the end of this study, it was concluded that monitoring Brucella isolates and identify them are indispensable epidemiological tool for brucellosis control
Atypical Brucellae, Bruce-ladder PCR, Brucella typing, Rev 1
Part of this study has been presented in Brucellosis 2011 International Research Conference, Including 64th Research Conference, 21-23 September, Buenos Aires, Argentina.
Birincil Dil | İngilizce |
---|---|
Konular | Veteriner Cerrahi |
Bölüm | Araştırma Makalesi |
Yazarlar |
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Yayımlanma Tarihi | 30 Haziran 2022 |
Yayınlandığı Sayı | Yıl 2022Cilt: 69 Sayı: 3 |
Bibtex | @araştırma makalesi { auvfd796785, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2022}, volume = {69}, number = {3}, pages = {297 - 302}, doi = {10.33988/auvfd.796785}, title = {Conventional and molecular identification of Brucella isolates from livestock in Turkey}, key = {cite}, author = {Erdenlig Gürbilek, Sevil and Baklan, Emin Ayhan and Sağlam, Gülnur and Karagül, Mustafa Sencer and Saytekin, Ahmet Murat} } |
APA | Erdenlig Gürbilek, S. , Baklan, E. A. , Sağlam, G. , Karagül, M. S. & Saytekin, A. M. (2022). Conventional and molecular identification of Brucella isolates from livestock in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (3) , 297-302 . DOI: 10.33988/auvfd.796785 |
MLA | Erdenlig Gürbilek, S. , Baklan, E. A. , Sağlam, G. , Karagül, M. S. , Saytekin, A. M. "Conventional and molecular identification of Brucella isolates from livestock in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 297-302 <http://vetjournal.ankara.edu.tr/tr/pub/issue/70947/796785> |
Chicago | Erdenlig Gürbilek, S. , Baklan, E. A. , Sağlam, G. , Karagül, M. S. , Saytekin, A. M. "Conventional and molecular identification of Brucella isolates from livestock in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 297-302 |
RIS | TY - JOUR T1 - Conventional and molecular identification of Brucella isolates from livestock in Turkey AU - SevilErdenlig Gürbilek, Emin AyhanBaklan, GülnurSağlam, Mustafa SencerKaragül, Ahmet MuratSaytekin Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.796785 DO - 10.33988/auvfd.796785 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 297 EP - 302 VL - 69 IS - 3 SN - 1300-0861-1308-2817 M3 - doi: 10.33988/auvfd.796785 UR - https://doi.org/10.33988/auvfd.796785 Y2 - 2021 ER - |
EndNote | %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Conventional and molecular identification of Brucella isolates from livestock in Turkey %A Sevil Erdenlig Gürbilek , Emin Ayhan Baklan , Gülnur Sağlam , Mustafa Sencer Karagül , Ahmet Murat Saytekin %T Conventional and molecular identification of Brucella isolates from livestock in Turkey %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 69 %N 3 %R doi: 10.33988/auvfd.796785 %U 10.33988/auvfd.796785 |
ISNAD | Erdenlig Gürbilek, Sevil , Baklan, Emin Ayhan , Sağlam, Gülnur , Karagül, Mustafa Sencer , Saytekin, Ahmet Murat . "Conventional and molecular identification of Brucella isolates from livestock in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 3 (Haziran 2022): 297-302 . https://doi.org/10.33988/auvfd.796785 |
AMA | Erdenlig Gürbilek S. , Baklan E. A. , Sağlam G. , Karagül M. S. , Saytekin A. M. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Univ Vet Fak Derg. 2022; 69(3): 297-302. |
Vancouver | Erdenlig Gürbilek S. , Baklan E. A. , Sağlam G. , Karagül M. S. , Saytekin A. M. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(3): 297-302. |
IEEE | S. Erdenlig Gürbilek , E. A. Baklan , G. Sağlam , M. S. Karagül ve A. M. Saytekin , "Conventional and molecular identification of Brucella isolates from livestock in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 69, sayı. 3, ss. 297-302, Haz. 2022, doi:10.33988/auvfd.796785 |