Araştırma Makalesi
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Conventional and molecular identification of Brucella isolates from livestock in Turkey

Yıl 2022, Cilt 69, Sayı 3, 297 - 302, 30.06.2022
https://doi.org/10.33988/auvfd.796785

Öz

The main object of the study is to demonstrate that Bruce-ladder multiplex PCR, which is capable of identify all Brucella species including vaccinal strains, is a safe and practical method that is alternative to bacteriological culture methods. Brucellosis is a zoonosis of great socio-economic importance which is endemic in Turkey. In three-year period from 2009 to 2011, a total of 281 Brucella spp. isolates from cattle, sheep and goats were identified and characterized by both conventional biotyping procedures and the PCR based method called Bruce-ladder. Species identification from both phenotypic testing and Bruce-ladder was concordant for all isolates tested. The results showed preferred host pattern in B.abortus isolates. In terms of species identity, it was found that 94% of the isolations derived from cattle were B.abortus and were exclusively biotype 3. B.melitensis biotype 3 and biotype 1 were the prevelant biotypes in sheep and goats. Two isolates from sheep were identified as B.melitensis Rev.1 vaccine strain. Surprisingly, one of the goat isolates was identified as B.abortus S19 vaccine strain. Other interesting observations were the isolation of penicilin-sensitive non vaccine strains of B.abortus and B.melitensis as well as an isolation of an atypical B.abortus biotype 3 strain on the basis of growth inhibition in the presence of basic fuchsin dye. At the end of this study, it was concluded that monitoring Brucella isolates and identify them are indispensable epidemiological tool for brucellosis control

Kaynakça

  • Aldomy FMM, Jahans KL, Altarzi YH (1992): Isolation of Brucella melitensis from aborting ruminants in Jordan. J Comp Path, 107, 239-242.
  • Alton GG, Jones LM, Angus RD, et al (1988): Techniques for the Brucellosis Laboratory. INRA, Paris, France.
  • Banai M, Mayer I, Cohen A (1990): Isolation, identification, and characterization in Israel of Brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. J Clin Microbiol, 28, 1057-1059.
  • Blasco JM (1997): A review of the use of B.melitensis Rev 1 vaccine in adult sheep and goats. Prev Vet Med, 31, 275-283.
  • Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus bv. 1,2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660-2666.
  • Büyükçangaz E, Şen A, Kahya S (2009): Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turk J Vet Anim Sci, 33, 311-316.
  • Corbel MJ (1989): Brucellosis: epidemiology and prevalence worldwide. 25-40. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1 st ed., Boca Raton, FL, USA.
  • Corbel MJ (1989): Microbiology of the genus Brucella. 54-67. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1st ed., Boca Raton, FL, USA.
  • Corbel MJ (1991): Identification of dye-sensitive strains of Brucella melitensis. J Clin Microbiol, 29, 1066-1068.
  • Corbel MJ (1997): Brucellosis:an overwiev. Emerg Infect Dis, 3, 213-221.
  • Erdenlig S, Şen A (2000): Koyun atıklarından izole edilen Brucella cinsi mikroorganizmaların izolasyonu ve biyotiplendirilmesi. Pendik Vet Mikrobiol Derg, 31, 31-42.
  • Erdenlig S, Iyisan AS, Baklan EA, et al (2007): Biovar distrubution of Brucella isolates from livestock in Turkey, 1999 to 2006. 27-28. In: Proceedings of the 15th International Congress of Mediterrenean Federation for Health and Production of Ruminants. Kuşadası, Turkey.
  • Ewalt DR, Forbes LB (1987): Atypical isolates of Brucella abortus from Canada and the United States characterized as dye sensitive with M antigen dominant. J Clin Microbiol, 25, 698-701.
  • Foster G, Osterman BS, Godfroid J, et al (2007): Brucella ceti sp. nov. Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol, 57, 2688–2693.
  • Garcia-Yoldi D, Marin CM, De Miguel MJ, et al (2006): Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem, 52, 779–781.
  • Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol, 93, 53-61.
  • Hemashettar BM, Patil CS, Jayakumar K, et al (1987): Isolation of Brucella melitensis biotype 1 from a cow and two of its attenders. Indian Vet J, 64, 822-825.
  • Ica T, Aydın F, Erdenliğ S, et al (2008): Characterisation of Brucella abortus biovar 3 isolates from Turkey as biovar 3b. The Vet Rec, 29, 660-662.
  • Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019): Chapter 3.1.4. Brucellosis (Brucella abortus, B. melitensis and B. suis) (infection with B. abortus, B. melitensis and B. suis) (NB: Version adopted in May2016), Available at https://www.oie.int/fileadmin/ Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf; [Accessed August 19, 2020].
  • Mayer-Scholl A, Draeger A, Göllner C, et al (2010): Advancement of a multiplex PCR for the differentiation of all currently described Brucella species. J Microbiol Method, 80, 112-114.
  • Ocholi RA, Kwaga JKP, Ajogi I, et al (2005): Abortion due to Brucella abortus in sheep in Nigeria. Rev Sci Tech Int Epiz, 24, 973-979.
  • Saytekin AM, Ak S (2018): Direct diagnosis of Brucella species through multiplex PCR formed by a new method. J Microbiol Method, 154, 86-94.
  • Scholz HC, Hubalek Z, Sedlacek I, et al (2008): Brucella microti sp. nov., isolated from the commonvole Microtus arvalis. Int J Syst Evol Microbiol, 58, 375–382.
  • Shaw WB (1976): Brucella abortus infection in sheep. I. Field case. Br Vet J, 132, 18-26.
  • Stinebring WR, Kunkel JR (1982): In vitro susceptibility of selected isolates of Brucella abortus to penicilin. Am J Vet Res, 43, 545-547.
  • Şahin M, Genç O, Ünver A, et al (2008): Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Product, 40, 281-286.

Yıl 2022, Cilt 69, Sayı 3, 297 - 302, 30.06.2022
https://doi.org/10.33988/auvfd.796785

Öz

Kaynakça

  • Aldomy FMM, Jahans KL, Altarzi YH (1992): Isolation of Brucella melitensis from aborting ruminants in Jordan. J Comp Path, 107, 239-242.
  • Alton GG, Jones LM, Angus RD, et al (1988): Techniques for the Brucellosis Laboratory. INRA, Paris, France.
  • Banai M, Mayer I, Cohen A (1990): Isolation, identification, and characterization in Israel of Brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. J Clin Microbiol, 28, 1057-1059.
  • Blasco JM (1997): A review of the use of B.melitensis Rev 1 vaccine in adult sheep and goats. Prev Vet Med, 31, 275-283.
  • Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus bv. 1,2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660-2666.
  • Büyükçangaz E, Şen A, Kahya S (2009): Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses. Turk J Vet Anim Sci, 33, 311-316.
  • Corbel MJ (1989): Brucellosis: epidemiology and prevalence worldwide. 25-40. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1 st ed., Boca Raton, FL, USA.
  • Corbel MJ (1989): Microbiology of the genus Brucella. 54-67. In: Young EJ, Corbel MJ (Ed), Brucellosis: Clinical and Laboratory Aspects. CRC Press 1st ed., Boca Raton, FL, USA.
  • Corbel MJ (1991): Identification of dye-sensitive strains of Brucella melitensis. J Clin Microbiol, 29, 1066-1068.
  • Corbel MJ (1997): Brucellosis:an overwiev. Emerg Infect Dis, 3, 213-221.
  • Erdenlig S, Şen A (2000): Koyun atıklarından izole edilen Brucella cinsi mikroorganizmaların izolasyonu ve biyotiplendirilmesi. Pendik Vet Mikrobiol Derg, 31, 31-42.
  • Erdenlig S, Iyisan AS, Baklan EA, et al (2007): Biovar distrubution of Brucella isolates from livestock in Turkey, 1999 to 2006. 27-28. In: Proceedings of the 15th International Congress of Mediterrenean Federation for Health and Production of Ruminants. Kuşadası, Turkey.
  • Ewalt DR, Forbes LB (1987): Atypical isolates of Brucella abortus from Canada and the United States characterized as dye sensitive with M antigen dominant. J Clin Microbiol, 25, 698-701.
  • Foster G, Osterman BS, Godfroid J, et al (2007): Brucella ceti sp. nov. Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol, 57, 2688–2693.
  • Garcia-Yoldi D, Marin CM, De Miguel MJ, et al (2006): Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem, 52, 779–781.
  • Güler L, Gündüz K, Ok U (2003): Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Vet Microbiol, 93, 53-61.
  • Hemashettar BM, Patil CS, Jayakumar K, et al (1987): Isolation of Brucella melitensis biotype 1 from a cow and two of its attenders. Indian Vet J, 64, 822-825.
  • Ica T, Aydın F, Erdenliğ S, et al (2008): Characterisation of Brucella abortus biovar 3 isolates from Turkey as biovar 3b. The Vet Rec, 29, 660-662.
  • Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2019): Chapter 3.1.4. Brucellosis (Brucella abortus, B. melitensis and B. suis) (infection with B. abortus, B. melitensis and B. suis) (NB: Version adopted in May2016), Available at https://www.oie.int/fileadmin/ Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf; [Accessed August 19, 2020].
  • Mayer-Scholl A, Draeger A, Göllner C, et al (2010): Advancement of a multiplex PCR for the differentiation of all currently described Brucella species. J Microbiol Method, 80, 112-114.
  • Ocholi RA, Kwaga JKP, Ajogi I, et al (2005): Abortion due to Brucella abortus in sheep in Nigeria. Rev Sci Tech Int Epiz, 24, 973-979.
  • Saytekin AM, Ak S (2018): Direct diagnosis of Brucella species through multiplex PCR formed by a new method. J Microbiol Method, 154, 86-94.
  • Scholz HC, Hubalek Z, Sedlacek I, et al (2008): Brucella microti sp. nov., isolated from the commonvole Microtus arvalis. Int J Syst Evol Microbiol, 58, 375–382.
  • Shaw WB (1976): Brucella abortus infection in sheep. I. Field case. Br Vet J, 132, 18-26.
  • Stinebring WR, Kunkel JR (1982): In vitro susceptibility of selected isolates of Brucella abortus to penicilin. Am J Vet Res, 43, 545-547.
  • Şahin M, Genç O, Ünver A, et al (2008): Investigation of bovine brucellosis in the Northeastern Turkey. Trop Anim Health Product, 40, 281-286.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Hekimlik
Bölüm Araştırma Makalesi
Yazarlar

Sevil ERDENLİG GÜRBİLEK>
Harran Üniversitesi, Veteriner Fakültesi
0000-0002-0377-2650
Türkiye


Emin Ayhan BAKLAN Bu kişi benim
Pendik Veteriner Kontrol Enstitüsü Müdürlüğü
0000-0001-5411-6234
Türkiye


Gülnur SAĞLAM>
Samsun Veteriner Kontrol Enstitüsü Müdürlüğü
0000-0001-7162-2577
Türkiye


Mustafa Sencer KARAGÜL>
KOCAELİ VOCATIONAL SCHOOL
0000-0001-7215-5229
Türkiye


Ahmet Murat SAYTEKİN> (Sorumlu Yazar)
Harran Üniversitesi, Veteriner Fakültesi
0000-0001-7486-8054
Türkiye

Teşekkür Part of this study has been presented in Brucellosis 2011 International Research Conference, Including 64th Research Conference, 21-23 September, Buenos Aires, Argentina.
Yayımlanma Tarihi 30 Haziran 2022
Yayınlandığı Sayı Yıl 2022, Cilt 69, Sayı 3

Kaynak Göster

Bibtex @araştırma makalesi { auvfd796785, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, eissn = {1308-2817}, address = {}, publisher = {Ankara Üniversitesi}, year = {2022}, volume = {69}, number = {3}, pages = {297 - 302}, doi = {10.33988/auvfd.796785}, title = {Conventional and molecular identification of Brucella isolates from livestock in Turkey}, key = {cite}, author = {Erdenlig Gürbilek, Sevil and Baklan, Emin Ayhan and Sağlam, Gülnur and Karagül, Mustafa Sencer and Saytekin, Ahmet Murat} }
APA Erdenlig Gürbilek, S. , Baklan, E. A. , Sağlam, G. , Karagül, M. S. & Saytekin, A. M. (2022). Conventional and molecular identification of Brucella isolates from livestock in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 69 (3) , 297-302 . DOI: 10.33988/auvfd.796785
MLA Erdenlig Gürbilek, S. , Baklan, E. A. , Sağlam, G. , Karagül, M. S. , Saytekin, A. M. "Conventional and molecular identification of Brucella isolates from livestock in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 297-302 <http://vetjournal.ankara.edu.tr/tr/pub/issue/70947/796785>
Chicago Erdenlig Gürbilek, S. , Baklan, E. A. , Sağlam, G. , Karagül, M. S. , Saytekin, A. M. "Conventional and molecular identification of Brucella isolates from livestock in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 (2022 ): 297-302
RIS TY - JOUR T1 - Conventional and molecular identification of Brucella isolates from livestock in Turkey AU - SevilErdenlig Gürbilek, Emin AyhanBaklan, GülnurSağlam, Mustafa SencerKaragül, Ahmet MuratSaytekin Y1 - 2022 PY - 2022 N1 - doi: 10.33988/auvfd.796785 DO - 10.33988/auvfd.796785 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 297 EP - 302 VL - 69 IS - 3 SN - -1308-2817 M3 - doi: 10.33988/auvfd.796785 UR - https://doi.org/10.33988/auvfd.796785 Y2 - 2021 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Conventional and molecular identification of Brucella isolates from livestock in Turkey %A Sevil Erdenlig Gürbilek , Emin Ayhan Baklan , Gülnur Sağlam , Mustafa Sencer Karagül , Ahmet Murat Saytekin %T Conventional and molecular identification of Brucella isolates from livestock in Turkey %D 2022 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P -1308-2817 %V 69 %N 3 %R doi: 10.33988/auvfd.796785 %U 10.33988/auvfd.796785
ISNAD Erdenlig Gürbilek, Sevil , Baklan, Emin Ayhan , Sağlam, Gülnur , Karagül, Mustafa Sencer , Saytekin, Ahmet Murat . "Conventional and molecular identification of Brucella isolates from livestock in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 69 / 3 (Haziran 2022): 297-302 . https://doi.org/10.33988/auvfd.796785
AMA Erdenlig Gürbilek S. , Baklan E. A. , Sağlam G. , Karagül M. S. , Saytekin A. M. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Univ Vet Fak Derg. 2022; 69(3): 297-302.
Vancouver Erdenlig Gürbilek S. , Baklan E. A. , Sağlam G. , Karagül M. S. , Saytekin A. M. Conventional and molecular identification of Brucella isolates from livestock in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2022; 69(3): 297-302.
IEEE S. Erdenlig Gürbilek , E. A. Baklan , G. Sağlam , M. S. Karagül ve A. M. Saytekin , "Conventional and molecular identification of Brucella isolates from livestock in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 69, sayı. 3, ss. 297-302, Haz. 2022, doi:10.33988/auvfd.796785