Araştırma Makalesi
BibTex RIS Kaynak Göster

Effect of ascorbic acid on collagen and some inflammatory mediators in rats

Yıl 2023, Cilt: 70 Sayı: 3, 303 - 308, 23.06.2023
https://doi.org/10.33988/auvfd.1019201

Öz

Musculoskeletal injuries as a kind of trauma that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study aimed to evaluate the effects of dose-dependent ascorbic acid (AA) administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomly divided into 6 groups of 11 each [control, muscle injury, healthy (with 5 mg/10 mg/kg/day AA-treated group), injury (with 5 mg/10 mg/kg/day AA-treated group)]. A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. AA (5-10 mg/kg/day) was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that a dose of 5 mg/kg/day AA administration reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain the mechanism detailed molecular analyzes are needed.

Destekleyen Kurum

This study was financially supported by Ankara University Scientific Research Projects Coordinator (Project number: 19L0230013).

Proje Numarası

19L0230013

Teşekkür

This manuscript is based on master’s thesis of Sibel IŞIN.

Kaynakça

  • Aguiló A, Monjo M, Moreno C, et al (2014): Vitamin C supplementation does not influence plasma and blood mononuclear cell IL-6 and IL-10 levels after exercise. J Sports Sci, 32, 1659-1669.
  • Agyare C, Osafo N, Boakye YD (2018): Biomarkers of Wound Healing. 23-31. In: KH Dogan (Ed), Wound Healing-Current Perspectives. IntechOpen, London.
  • Al Jameil N, Tabassum H, Fatima S, et al (2017): Ameliorating Effect of Vitamin C Against Potassium Dichromate Induced Oxidative Stress and Inflammatory Response in Rats. Int J Pharmacol, 13, 990-999.
  • Boncompagni S, Kern H, Rossini K, et al (2007): Structural differentiation of skeletal muscle fibers in the absence of innervation in humans. Proc Natl Acad Sci U S A, 104, 19339-19344.
  • Bowie AG, O’Neill LA (2000): Vitamin C inhibits NF-κB activation by TNF via the activation of p38 mitogen-activated protein kinase. J Immunol, 165, 7180-7188.
  • Bozonet SM, Carr AC, Pullar JM, et al (2015): Enhanced human neutrophil vitamin C status, chemotaxis and oxidant generation following dietary supplementation with vitamin C-rich SunGold kiwifruit. Nutrients, 7, 2574-2588.
  • Butowicz CM, Dearth CL, Hendershot BD (2017): Impact of traumatic lower extremity injuries beyond acute care: Movement-based considerations for resultant longer term secondary health conditions. Adv Wound Care (New Rochelle), 6, 269-278.
  • Carr AC, Maggini S (2017): Vitamin C and immune function. Nutrients, 9, 1211.
  • Catani MV, Savini I, Rossi A, et al (2005): Biological role of vitamin C in keratinocytes. Nutr Rev, 63, 81-90.
  • Ellulu MS, Rahmat A, Patimah I, et al (2015): Effect of vitamin C on inflammation and metabolic markers in hypertensive and/or diabetic obese adults: a randomized controlled trial. Drug Des Devel Ther, 9, 3405-3412.
  • Fu SC, Cheng WH, Cheuk YC, et al (2013): Development of vitamin C irrigation saline to promote graft healing in anterior cruciate ligament reconstruction. J Orthop Translat, 1, 67-77.
  • Gonzalez ACDO, Costa TF, Andrade ZDA, et al (2016): Wound healing-A literature review. An Bras Dermatol, 91, 614-620.
  • Guo SA, Dipietro LA (2010): Factors affecting wound healing. J Dent Res, 89, 219-229.
  • Huijskens MJAJ, Walczak M, Koller N, et al (2014): Technical Advance: Ascorbic acid induces development of double-positive T cells from human hematopoietic stem cells in the absence of stromal cells. J Leukoc Biol, 96, 1165-1175.
  • Hung LK, Fu SC, Lee YW, et al (2013): Local vitamin-C injection reduced tendon adhesion in a chicken model of flexor digitorum profundus tendon injury. JBJS, 95, e41.
  • Kessler B, Rinchai D, Kewcharoenwong C, et al (2017): Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep, 7, 1-11.
  • Kim AY, Lee EM, Lee EJ, et al (2013): Effects of vitamin C on cytotherapy-mediated muscle regeneration. Cell Transplant, 22, 1845-1858.
  • Kunduracioglu B, Ulkar B, Sabuncuoglu BT, et al (2006): Effects of hypertonic dextrose on injured rat skeletal muscles. Neurosciences Journal, 11, 93-96.
  • Lima CC, Pereira APC, Silva JRF, et al (2009): Ascorbic acid for the healing of skin wounds in rats. Braz J Biol, 69, 1195-1201.
  • Loizidis T, Sioga A, Economou L, et al (2007): The role of ascorbic acid and exercise in chronic ischemia of skeletal muscle in rats. J Appl Physiol (1985), 102, 321-330.
  • Mackay D, Miller AL (2003): Nutritional support for wound healing. Altern Med Rev, 8, 359-377.
  • Nieman DC, Peters EM, Henson DA, et al (2000): Influence of vitamin C supplementation on cytokine changes following an ultramarathon. J Interferon Cytokine Res, 20, 1029-1035.
  • Omeroğlu S, Peker T, Türközkan N, et al (2009): High-dose vitamin C supplementation accelerates the Achilles tendon healing in healthy rats. Arch Orthop Trauma Surg, 129, 281-286.
  • Su M, Liang X, Xu X, et al (2019): Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress. Environ Toxicol Pharmacol, 65, 60-65.
  • Takisawa S, Funakoshi T, Yatsu T, et al (2019): Vitamin C deficiency causes muscle atrophy and a deterioration in physical performance. Sci Rep, 9, 1-10.
  • Tang P, Zhu R, Gu Y, et al (2018): Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy after Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model. Spine, 43, E1249-E1259.
  • Tonon E, Ferretti R, Shiratori JH, et al (2012): Ascorbic acid protects the diaphragm muscle against myonecrosis in mdx mice. Nutrition, 28, 686-690.
  • Wassall HJH, Devereux G, Seaton A, et al (2013): Complex effects of vitamin E and vitamin C supplementation on in vitro neonatal mononuclear cell responses to allergens. Nutrients, 5, 3337-3351.
  • Wilkinson HN, Hardman MJ (2020): Wound healing: cellular mechanisms and pathological outcomes. Open Biol, 10, 200223.
  • Woolf AD, Pfleger B (2003): Burden of major musculoskeletal conditions. Bull World Health Organ, 81, 646-656.
  • Yun SH, Moon YS, Sohn SH, et al (2012): Effects of cyclic heat stress or vitamin C supplementation during cyclic heat stress on HSP70, inflammatory cytokines, and the antioxidant defense system in Sprague Dawley rats. Exp Anim, 61, 543-553.
Yıl 2023, Cilt: 70 Sayı: 3, 303 - 308, 23.06.2023
https://doi.org/10.33988/auvfd.1019201

Öz

Proje Numarası

19L0230013

Kaynakça

  • Aguiló A, Monjo M, Moreno C, et al (2014): Vitamin C supplementation does not influence plasma and blood mononuclear cell IL-6 and IL-10 levels after exercise. J Sports Sci, 32, 1659-1669.
  • Agyare C, Osafo N, Boakye YD (2018): Biomarkers of Wound Healing. 23-31. In: KH Dogan (Ed), Wound Healing-Current Perspectives. IntechOpen, London.
  • Al Jameil N, Tabassum H, Fatima S, et al (2017): Ameliorating Effect of Vitamin C Against Potassium Dichromate Induced Oxidative Stress and Inflammatory Response in Rats. Int J Pharmacol, 13, 990-999.
  • Boncompagni S, Kern H, Rossini K, et al (2007): Structural differentiation of skeletal muscle fibers in the absence of innervation in humans. Proc Natl Acad Sci U S A, 104, 19339-19344.
  • Bowie AG, O’Neill LA (2000): Vitamin C inhibits NF-κB activation by TNF via the activation of p38 mitogen-activated protein kinase. J Immunol, 165, 7180-7188.
  • Bozonet SM, Carr AC, Pullar JM, et al (2015): Enhanced human neutrophil vitamin C status, chemotaxis and oxidant generation following dietary supplementation with vitamin C-rich SunGold kiwifruit. Nutrients, 7, 2574-2588.
  • Butowicz CM, Dearth CL, Hendershot BD (2017): Impact of traumatic lower extremity injuries beyond acute care: Movement-based considerations for resultant longer term secondary health conditions. Adv Wound Care (New Rochelle), 6, 269-278.
  • Carr AC, Maggini S (2017): Vitamin C and immune function. Nutrients, 9, 1211.
  • Catani MV, Savini I, Rossi A, et al (2005): Biological role of vitamin C in keratinocytes. Nutr Rev, 63, 81-90.
  • Ellulu MS, Rahmat A, Patimah I, et al (2015): Effect of vitamin C on inflammation and metabolic markers in hypertensive and/or diabetic obese adults: a randomized controlled trial. Drug Des Devel Ther, 9, 3405-3412.
  • Fu SC, Cheng WH, Cheuk YC, et al (2013): Development of vitamin C irrigation saline to promote graft healing in anterior cruciate ligament reconstruction. J Orthop Translat, 1, 67-77.
  • Gonzalez ACDO, Costa TF, Andrade ZDA, et al (2016): Wound healing-A literature review. An Bras Dermatol, 91, 614-620.
  • Guo SA, Dipietro LA (2010): Factors affecting wound healing. J Dent Res, 89, 219-229.
  • Huijskens MJAJ, Walczak M, Koller N, et al (2014): Technical Advance: Ascorbic acid induces development of double-positive T cells from human hematopoietic stem cells in the absence of stromal cells. J Leukoc Biol, 96, 1165-1175.
  • Hung LK, Fu SC, Lee YW, et al (2013): Local vitamin-C injection reduced tendon adhesion in a chicken model of flexor digitorum profundus tendon injury. JBJS, 95, e41.
  • Kessler B, Rinchai D, Kewcharoenwong C, et al (2017): Interleukin 10 inhibits pro-inflammatory cytokine responses and killing of Burkholderia pseudomallei. Sci Rep, 7, 1-11.
  • Kim AY, Lee EM, Lee EJ, et al (2013): Effects of vitamin C on cytotherapy-mediated muscle regeneration. Cell Transplant, 22, 1845-1858.
  • Kunduracioglu B, Ulkar B, Sabuncuoglu BT, et al (2006): Effects of hypertonic dextrose on injured rat skeletal muscles. Neurosciences Journal, 11, 93-96.
  • Lima CC, Pereira APC, Silva JRF, et al (2009): Ascorbic acid for the healing of skin wounds in rats. Braz J Biol, 69, 1195-1201.
  • Loizidis T, Sioga A, Economou L, et al (2007): The role of ascorbic acid and exercise in chronic ischemia of skeletal muscle in rats. J Appl Physiol (1985), 102, 321-330.
  • Mackay D, Miller AL (2003): Nutritional support for wound healing. Altern Med Rev, 8, 359-377.
  • Nieman DC, Peters EM, Henson DA, et al (2000): Influence of vitamin C supplementation on cytokine changes following an ultramarathon. J Interferon Cytokine Res, 20, 1029-1035.
  • Omeroğlu S, Peker T, Türközkan N, et al (2009): High-dose vitamin C supplementation accelerates the Achilles tendon healing in healthy rats. Arch Orthop Trauma Surg, 129, 281-286.
  • Su M, Liang X, Xu X, et al (2019): Hepatoprotective benefits of vitamin C against perfluorooctane sulfonate-induced liver damage in mice through suppressing inflammatory reaction and ER stress. Environ Toxicol Pharmacol, 65, 60-65.
  • Takisawa S, Funakoshi T, Yatsu T, et al (2019): Vitamin C deficiency causes muscle atrophy and a deterioration in physical performance. Sci Rep, 9, 1-10.
  • Tang P, Zhu R, Gu Y, et al (2018): Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy after Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model. Spine, 43, E1249-E1259.
  • Tonon E, Ferretti R, Shiratori JH, et al (2012): Ascorbic acid protects the diaphragm muscle against myonecrosis in mdx mice. Nutrition, 28, 686-690.
  • Wassall HJH, Devereux G, Seaton A, et al (2013): Complex effects of vitamin E and vitamin C supplementation on in vitro neonatal mononuclear cell responses to allergens. Nutrients, 5, 3337-3351.
  • Wilkinson HN, Hardman MJ (2020): Wound healing: cellular mechanisms and pathological outcomes. Open Biol, 10, 200223.
  • Woolf AD, Pfleger B (2003): Burden of major musculoskeletal conditions. Bull World Health Organ, 81, 646-656.
  • Yun SH, Moon YS, Sohn SH, et al (2012): Effects of cyclic heat stress or vitamin C supplementation during cyclic heat stress on HSP70, inflammatory cytokines, and the antioxidant defense system in Sprague Dawley rats. Exp Anim, 61, 543-553.
Toplam 31 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi, Veteriner Hekimlik
Bölüm Araştırma Makalesi
Yazarlar

Sibel Işın 0000-0001-8492-0055

Nuray Yazıhan 0000-0003-1237-8468

Burcu Kesikli 0000-0003-2784-3684

Derya Biriken 0000-0002-6254-4578

Sevginur Akdaş 0000-0002-1769-4171

Ferda Topal Celikkan 0000-0002-8254-0558

Batuhan Bakırarar 0000-0002-5662-8193

Pelin Arıbal-ayral 0000-0002-5047-7881

Proje Numarası 19L0230013
Yayımlanma Tarihi 23 Haziran 2023
Yayımlandığı Sayı Yıl 2023Cilt: 70 Sayı: 3

Kaynak Göster

APA Işın, S., Yazıhan, N., Kesikli, B., Biriken, D., vd. (2023). Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 70(3), 303-308. https://doi.org/10.33988/auvfd.1019201
AMA Işın S, Yazıhan N, Kesikli B, Biriken D, Akdaş S, Topal Celikkan F, Bakırarar B, Arıbal-ayral P. Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Univ Vet Fak Derg. Haziran 2023;70(3):303-308. doi:10.33988/auvfd.1019201
Chicago Işın, Sibel, Nuray Yazıhan, Burcu Kesikli, Derya Biriken, Sevginur Akdaş, Ferda Topal Celikkan, Batuhan Bakırarar, ve Pelin Arıbal-ayral. “Effect of Ascorbic Acid on Collagen and Some Inflammatory Mediators in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70, sy. 3 (Haziran 2023): 303-8. https://doi.org/10.33988/auvfd.1019201.
EndNote Işın S, Yazıhan N, Kesikli B, Biriken D, Akdaş S, Topal Celikkan F, Bakırarar B, Arıbal-ayral P (01 Haziran 2023) Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70 3 303–308.
IEEE S. Işın, N. Yazıhan, B. Kesikli, D. Biriken, S. Akdaş, F. Topal Celikkan, B. Bakırarar, ve P. Arıbal-ayral, “Effect of ascorbic acid on collagen and some inflammatory mediators in rats”, Ankara Univ Vet Fak Derg, c. 70, sy. 3, ss. 303–308, 2023, doi: 10.33988/auvfd.1019201.
ISNAD Işın, Sibel vd. “Effect of Ascorbic Acid on Collagen and Some Inflammatory Mediators in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 70/3 (Haziran 2023), 303-308. https://doi.org/10.33988/auvfd.1019201.
JAMA Işın S, Yazıhan N, Kesikli B, Biriken D, Akdaş S, Topal Celikkan F, Bakırarar B, Arıbal-ayral P. Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Univ Vet Fak Derg. 2023;70:303–308.
MLA Işın, Sibel vd. “Effect of Ascorbic Acid on Collagen and Some Inflammatory Mediators in Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, c. 70, sy. 3, 2023, ss. 303-8, doi:10.33988/auvfd.1019201.
Vancouver Işın S, Yazıhan N, Kesikli B, Biriken D, Akdaş S, Topal Celikkan F, Bakırarar B, Arıbal-ayral P. Effect of ascorbic acid on collagen and some inflammatory mediators in rats. Ankara Univ Vet Fak Derg. 2023;70(3):303-8.