Research Article
Effects of conventional and organic rearing systems and hen age on oxidative stress parameters of blood and ovarian tissues in laying hens
Abstract
In this study, effects of hen age and rearing systems on some oxidative stress parameters in serum and ovarian tissues of hens are investigated. For this purpose, total of 48 Bovans White commercial laying hybrids from two different rearing systems (conventional cage and organic) at 30 and 60 weeks of age were used. Serum and ovarian tissues of the hens were collected following slaughter process and malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-Px) and catalase (CAT) levels of serum and ovarian tissues were determined. MDA levels of serum was found significantly higher in conventional system, both in 30 and 60 weeks old (P<0.05). GSH-Px activity was lower, conversely CAT activity was significantly higher in conventional system (P<0.05). Hen age didn’t affect MDA, GSH, GSH-Px and CAT levels of serum (P>0.05). In addition, there weren’t any significant interactions between rearing system and hen age in serum (P>0.05). MDA (P=0.010) and GSH (P<0.01) levels of ovarian tissue were found significantly higher in conventional system while GSH levels of ovarian tissue had increased in 60 weeks old (P<0.01). GSH-Px and CAT activity was similar between groups (P>0.05). Interaction between rearing system and hen age affected activity of CAT in ovarian tissue (P<0.05). As a result, conventional battery cages caused oxidative stress interrelated with increasing lipid peroxidation and MDA levels in laying hens. Antioxidant enzymes that play role in reduction of MDA were inadequate in this system. Oxidant/antioxidant balance can be established at 30 or 60 weeks of age by hen organism and therefore MDA levels of tissues would not be affected by age
References
- Aebi H (1984): Methods of Enzymatic Analysis. 671-684. In: HU Bergmeyer (Ed), Catalase. Academic Press, London.
- Anderson KE (2011): Comparison of fatty acid, cholesterol, and vitamin A and E composition in eggs from hens housed in conventional cage and range production facilities. Poult Sci, 90, 1600-1608.
- AOAC (1995): Official methods of analysis: 16th ed. Arlington.
- Appleby MC (2003): The European Union ban on conventional cages for laying hens: History and prospects. J Appl Anim Welf Sci, 6, 103-121.
- Atmaca N, Çınar M, Güner B, et al. (2015): Evaluation of oxidative parameters during Toxoplasma gondii infection in gerbils. Ankara Üniv Vet Fak Derg, 62, 165-170. biochemical
- Balin AK, Allen RG (1986): Mechanisms of biologic aging. Dermatol Clin, 4, 347-358.
- Barbieri E, Sestili P (2012): Reactive oxygen species in skeletal muscle signaling. J Signal Transduct, Article ID 982794, 17.
- Başbakanlık Mevzuatı Geliştirme ve Yayın Genel Müdürlüğü (2011): Çiftlik Hayvanlarının Refahına İlişkin Yönetmelik. T.C. Resmi Gazete, 28151.
- Chavan S, Sava L, Saxena V, et al. (2005): Reduced Glutathione: Importance of specimen collection. I J of Clin Biochem, 20, 150-152.
- Cheng HW (2010): Breeding of tomorrow’s chickens to improve well-being. Poult Sci, 89, 805-813.
- Cole GM, Teter B, Frautschy SA (2007): Neuroprotective effects of curcumin. Adv Exp Med Biol, 595, 197-212.
- Cooper JJ, Albentosa MJ (2003): Behavioural priorities of laying hens. Avian Poult Biol Rev, 14, 127-149.
- David B, Moe RO, Michel V, et al. (2015): Air quality in alternative housing systems may have an impact on laying hen welfare. Part I-Dust. Animals (Basel), 5, 495-511.
- Downing J (2012): Non-invasive assessment of stress in commercial housing systems. AECL Publication No US108A, Australia.
- Englmaierová M, Tůmová E, Charvátová V, et al. (2014): Effects of laying hens housing system on laying performance, egg quality characteristics, and egg microbial contamination. Czech J Anim Sci, 59, 345–352.
- Erişir M, Erişir Z, Seyran A (2010): Farklı yetiştirme sistemlerinin pekin ördeklerindeki plazma malondialdehit, retinol ve β‐karoten düzeyleri üzerine etkisi. Atatürk Üniversitesi Vet Bil Derg, 5, 21‐25.
- European Union (1997): Treaty of Amsterdam Amending the Treaty on European Union, the Treaties Establishing the European Communities and Certain Related Acts. Official Journal of European Communities, C 340, 110.
- European Union (1999): Production of organic livestock (supplementing EU Regulation 2092/91). Official Journal of European Communities, L 222, 13-28.
- Fairbanks VF, Klee GG (1986): Biochemical aspects of hematology. 1532-1534. In: NW Tietz (Ed), Textbook of Clinical Chemistry. WB Saunders Company, Philadelphia.
- Grotto D, Santa Maria L, Valentini J, et al. (2009): Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim Nova, 32, 169-174.
- Kandemir FM, Erişir M, Yüksel M (2016): Comparison of lipid peroxidation and several antioxidants in blood of normally calved and dystocia affected cows and their newborn calves. Isr J Vet Med, 71, 19-23.
- Karagöz Y (2015): Etkileşimli (İnteraksiyonlu) Varyans Analizi. 277-284. Güncellenmiş 2. Basım, SPSS 22 Uygulamalı Biyoistatistik. Nobel, Ankara.
- Küçükyılmaz K, Bozkurt M, Yamaner Ç, et al. (2012): Effect of an organic and conventional rearing system on the mineral content of hen eggs. Food Chem, 132, 989-992.
- Lay Jr. DC, Fulton RM, Hester PY, et al. (2011): Hen welfare in different housing systems. Poult Sci, 90, 278-294.
- Lourenço dos Santos S, Baraibar MA, Lundberg S, et al. (2015): Oxidative proteome alterations during skeletal muscle ageing. Redox Biol, 5, 267-274.
- Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951): Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
- Matkovics B, Szabo I, Varga IS (1988): Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15, 248–249.
- Matur E, Akyazı İ, Eraslan E, et al. (2016): The effects of environmental enrichment and transport stress on the weights of lymphoid organs, cell-mediated immune response, heterophil functions and antibody production in laying hens. Anim Sci J, 87, 284-292.
- Mench JA, Van Tienhoven JA, Marsh CC, et al. (1986): Effects of cage and floor pen management on behavior, production and physiological stress responses of laying hens. Poult Sci, 65, 1058-1069.
- Mugnai C, Dal Bosco A, Moscati L, et al. (2011): Effect of genotype and husbandry system on blood parameters, oxidative and native immune status: Welfare and implications on performance of organic laying hens. Open Vet Sci J, 5, 12-18.
- Nicol CJ (1987): Behavioural responses of laying hens following a period of spatial restriction. Anim Behav, 35, 1709-1719.
- NRC (1994): Nutrient Requirements of Poultry. 9th rev ed, Natl. Acad. Press, Washington, DC.
- Ognik K, Czech A, Stachyra K (2013): Effect of a natural versus a synthetic antioxidant, and sex and age on the redox profile in the blood of growing turkeys. S Afr J Anim Sci, 43, 473-481.
- Petrik MT, Guerin MT, Widowski TM (2015): On-farm comparison of keel fracture prevalence and other welfare indicators in conventional cage and floor-housed laying hens in Ontario, Canada. Poult Sci, 94, 579-585.
- Placer ZA, Cushman LL, Johson BC (1966): Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem 16, 359–364.
- Pohle H, Cheng HW (2009): Comparative effects of furnished and battery cages on egg production and physiological parameters in White Leghorn hens. Poult Sci, 88, 2042-2051.
- Rahman K (2007): Studies on free radicals, antioxidants, and co-factors. Clin Interv Aging, 2, 219-236.
- Rodenburg TB, Tuyttens FAM, Sonck B, et al. (2005): Welfare, health, and hygiene of laying hens housed in furnished cages and in alternative housing systems. J Appl Anim Welf Sci, 8, 211-226.
- Shini S (2003): Physiological responses of laying hens to the alternative housing systems. Int J Poult Sci, 2, 357-360.
- Sies H (1999): Glutathione and its role in cellular functions. Free Radic Biol Med, 27, 916-921.
- Şimşek ÜG, Çiftçi M, Doğan G, et al. (2013): Antioxidant activity of cinnamon bark oil (cinnamomum zeylanicum l.) in Japanese quails under thermo neutral and heat stressed conditions. Kafkas Univ Vet Fak Derg, 19, 889-894.
- Sohal RS, Weindruch R (1996): Oxidative stress, caloric restriction, and aging. National Institutes of Health, 273, 59-63.
- Tactacan GB, Guenter W, Lewis NJ, et al. (2009): Performance and welfare of laying hens in conventional and enriched cages. Poult Sci, 88, 698-707.
- Tauson R, Wahlström A, Abrahamsson P (1999): Effect of two floor housing systems and cages on health, production, and fear response in layers. J Appl Poult Res, 8, 152-159.
- Toscano MJ, Booth F, Wilkins LJ, et al. (2015): The effects of long (C20/22) and short (C18) chain omega-3 fatty acids on keel bone fractures, bone biomechanics, behavior, and egg production in free-range laying hens. Poult Sci, 94, 823–835.
- Tsutsui H, Kinugawa S, Matsushima S (2011): Oxidative stress and heart failure. Am J Physiol Heart Circ Physiol, 301, 2181-2190.
- United Egg Producers UEP Certified Program (2010): Animal husbandry guide lines for U.S. egg laying flocks 2010 Edition.
- Venardos K, Harrison G, Headrick J, et al. (2004): Effects of dietary selenium on glutathione peroxidase and thioredoxin reductase activity and recovery from cardiac ischemia-reperfusion. J Trace Elem Med Biol, 18, 81-88.
- Verma PK, Singh Y, Raina R, et al. (2012): Stress biomarkers in Vanaraja chicken maintained under various rearing systems. J Advan Vet Res, 2, 5-8.
- Wang XJ, Li Y, Song QQ, et al. (2013): Corticosterone regulation of ovarian follicular development is dependent on the energy status of laying hens. J Lipid Res, 54, 1860-
Konvansiyonel ve organik yetiştirme sistemleri ile tavuk yaşının yumurta tavuklarının kan veyumurtalık dokusundaki oksidatif stres parametreleri üzerine etkisi
Abstract
Bu çalışmada yetiştirme sistemleri ve tavuk yaşının, tavukların serum ve yumurtalık dokusundaki bazı oksidatif stres parametreleri üzerine etkileri incelendi. Bu amaçla 30 ve 60 haftalık yaşta, iki farklı sistemden (konvansiyonel kafes ve organik) toplam 48 adet ticari yumurtacı hibrit olan Bovans White kullanıldı. Kesim işlemini takiben tavukların serum ve yumurtalık dokuları toplanarak, malondialdehid (MDA), glutatyon (GSH), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) düzeyleri belirlendi. Konvansiyonel kafeste yetiştirilen hem 30, hem de 60 haftalık tavukların serum MDA düzeyleri önemli derecede yüksek bulundu (P<0.05). Konvansiyonel kafeste GSH-Px aktivitesi düşük, aksine CAT aktivitesi önemli düzeyde yüksek tespit edildi (P<0.05). Tavuk yaşı serum MDA, GSH, GSH-Px ve CAT düzeylerini etkilemedi (P>0.05). Buna ek olarak, serumda, yetiştirme sistemi ve tavuk yaşı arasında önemli bir etkileşim bulunmadı (P>0.05). Konvansiyonel sistemdeki tavukların yumurtalık dokusunda MDA (P=0.010) ve GSH (P<0.01) seviyeleri önemli düzeyde yüksek bulunurken, GSH seviyesi 60 haftalık yaşta arttı (P<0.01). GSH-Px ve CAT aktiviteleri gruplar arasında benzer çıktı (P>0.05). Yetiştirme sistemi ve tavuk yaşı arasındaki etkileşim yumurtalık dokusunda CAT aktivitesini etkiledi (P<0.05). Sonuç olarak, konvansiyonel bataryalı kafesler, lipid peroksidasyon ve MDA düzeylerinin artmasıyla ilişkili olarak tavuklarda oksidatif strese neden oldu. Bu sistemde MDA’nın azalmasında rol oynayan antioksidan enzimler yetersiz kaldı. Oksidan/antioksidan dengesi 30 ve 60 haftalık yaşlarda tavuk organizması tarafından kurulabildi ve dolayısıyla dokuların MDA düzeyleri yaştan etkilenmedi
References
- Aebi H (1984): Methods of Enzymatic Analysis. 671-684. In: HU Bergmeyer (Ed), Catalase. Academic Press, London.
- Anderson KE (2011): Comparison of fatty acid, cholesterol, and vitamin A and E composition in eggs from hens housed in conventional cage and range production facilities. Poult Sci, 90, 1600-1608.
- AOAC (1995): Official methods of analysis: 16th ed. Arlington.
- Appleby MC (2003): The European Union ban on conventional cages for laying hens: History and prospects. J Appl Anim Welf Sci, 6, 103-121.
- Atmaca N, Çınar M, Güner B, et al. (2015): Evaluation of oxidative parameters during Toxoplasma gondii infection in gerbils. Ankara Üniv Vet Fak Derg, 62, 165-170. biochemical
- Balin AK, Allen RG (1986): Mechanisms of biologic aging. Dermatol Clin, 4, 347-358.
- Barbieri E, Sestili P (2012): Reactive oxygen species in skeletal muscle signaling. J Signal Transduct, Article ID 982794, 17.
- Başbakanlık Mevzuatı Geliştirme ve Yayın Genel Müdürlüğü (2011): Çiftlik Hayvanlarının Refahına İlişkin Yönetmelik. T.C. Resmi Gazete, 28151.
- Chavan S, Sava L, Saxena V, et al. (2005): Reduced Glutathione: Importance of specimen collection. I J of Clin Biochem, 20, 150-152.
- Cheng HW (2010): Breeding of tomorrow’s chickens to improve well-being. Poult Sci, 89, 805-813.
- Cole GM, Teter B, Frautschy SA (2007): Neuroprotective effects of curcumin. Adv Exp Med Biol, 595, 197-212.
- Cooper JJ, Albentosa MJ (2003): Behavioural priorities of laying hens. Avian Poult Biol Rev, 14, 127-149.
- David B, Moe RO, Michel V, et al. (2015): Air quality in alternative housing systems may have an impact on laying hen welfare. Part I-Dust. Animals (Basel), 5, 495-511.
- Downing J (2012): Non-invasive assessment of stress in commercial housing systems. AECL Publication No US108A, Australia.
- Englmaierová M, Tůmová E, Charvátová V, et al. (2014): Effects of laying hens housing system on laying performance, egg quality characteristics, and egg microbial contamination. Czech J Anim Sci, 59, 345–352.
- Erişir M, Erişir Z, Seyran A (2010): Farklı yetiştirme sistemlerinin pekin ördeklerindeki plazma malondialdehit, retinol ve β‐karoten düzeyleri üzerine etkisi. Atatürk Üniversitesi Vet Bil Derg, 5, 21‐25.
- European Union (1997): Treaty of Amsterdam Amending the Treaty on European Union, the Treaties Establishing the European Communities and Certain Related Acts. Official Journal of European Communities, C 340, 110.
- European Union (1999): Production of organic livestock (supplementing EU Regulation 2092/91). Official Journal of European Communities, L 222, 13-28.
- Fairbanks VF, Klee GG (1986): Biochemical aspects of hematology. 1532-1534. In: NW Tietz (Ed), Textbook of Clinical Chemistry. WB Saunders Company, Philadelphia.
- Grotto D, Santa Maria L, Valentini J, et al. (2009): Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim Nova, 32, 169-174.
- Kandemir FM, Erişir M, Yüksel M (2016): Comparison of lipid peroxidation and several antioxidants in blood of normally calved and dystocia affected cows and their newborn calves. Isr J Vet Med, 71, 19-23.
- Karagöz Y (2015): Etkileşimli (İnteraksiyonlu) Varyans Analizi. 277-284. Güncellenmiş 2. Basım, SPSS 22 Uygulamalı Biyoistatistik. Nobel, Ankara.
- Küçükyılmaz K, Bozkurt M, Yamaner Ç, et al. (2012): Effect of an organic and conventional rearing system on the mineral content of hen eggs. Food Chem, 132, 989-992.
- Lay Jr. DC, Fulton RM, Hester PY, et al. (2011): Hen welfare in different housing systems. Poult Sci, 90, 278-294.
- Lourenço dos Santos S, Baraibar MA, Lundberg S, et al. (2015): Oxidative proteome alterations during skeletal muscle ageing. Redox Biol, 5, 267-274.
- Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951): Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
- Matkovics B, Szabo I, Varga IS (1988): Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15, 248–249.
- Matur E, Akyazı İ, Eraslan E, et al. (2016): The effects of environmental enrichment and transport stress on the weights of lymphoid organs, cell-mediated immune response, heterophil functions and antibody production in laying hens. Anim Sci J, 87, 284-292.
- Mench JA, Van Tienhoven JA, Marsh CC, et al. (1986): Effects of cage and floor pen management on behavior, production and physiological stress responses of laying hens. Poult Sci, 65, 1058-1069.
- Mugnai C, Dal Bosco A, Moscati L, et al. (2011): Effect of genotype and husbandry system on blood parameters, oxidative and native immune status: Welfare and implications on performance of organic laying hens. Open Vet Sci J, 5, 12-18.
- Nicol CJ (1987): Behavioural responses of laying hens following a period of spatial restriction. Anim Behav, 35, 1709-1719.
- NRC (1994): Nutrient Requirements of Poultry. 9th rev ed, Natl. Acad. Press, Washington, DC.
- Ognik K, Czech A, Stachyra K (2013): Effect of a natural versus a synthetic antioxidant, and sex and age on the redox profile in the blood of growing turkeys. S Afr J Anim Sci, 43, 473-481.
- Petrik MT, Guerin MT, Widowski TM (2015): On-farm comparison of keel fracture prevalence and other welfare indicators in conventional cage and floor-housed laying hens in Ontario, Canada. Poult Sci, 94, 579-585.
- Placer ZA, Cushman LL, Johson BC (1966): Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem 16, 359–364.
- Pohle H, Cheng HW (2009): Comparative effects of furnished and battery cages on egg production and physiological parameters in White Leghorn hens. Poult Sci, 88, 2042-2051.
- Rahman K (2007): Studies on free radicals, antioxidants, and co-factors. Clin Interv Aging, 2, 219-236.
- Rodenburg TB, Tuyttens FAM, Sonck B, et al. (2005): Welfare, health, and hygiene of laying hens housed in furnished cages and in alternative housing systems. J Appl Anim Welf Sci, 8, 211-226.
- Shini S (2003): Physiological responses of laying hens to the alternative housing systems. Int J Poult Sci, 2, 357-360.
- Sies H (1999): Glutathione and its role in cellular functions. Free Radic Biol Med, 27, 916-921.
- Şimşek ÜG, Çiftçi M, Doğan G, et al. (2013): Antioxidant activity of cinnamon bark oil (cinnamomum zeylanicum l.) in Japanese quails under thermo neutral and heat stressed conditions. Kafkas Univ Vet Fak Derg, 19, 889-894.
- Sohal RS, Weindruch R (1996): Oxidative stress, caloric restriction, and aging. National Institutes of Health, 273, 59-63.
- Tactacan GB, Guenter W, Lewis NJ, et al. (2009): Performance and welfare of laying hens in conventional and enriched cages. Poult Sci, 88, 698-707.
- Tauson R, Wahlström A, Abrahamsson P (1999): Effect of two floor housing systems and cages on health, production, and fear response in layers. J Appl Poult Res, 8, 152-159.
- Toscano MJ, Booth F, Wilkins LJ, et al. (2015): The effects of long (C20/22) and short (C18) chain omega-3 fatty acids on keel bone fractures, bone biomechanics, behavior, and egg production in free-range laying hens. Poult Sci, 94, 823–835.
- Tsutsui H, Kinugawa S, Matsushima S (2011): Oxidative stress and heart failure. Am J Physiol Heart Circ Physiol, 301, 2181-2190.
- United Egg Producers UEP Certified Program (2010): Animal husbandry guide lines for U.S. egg laying flocks 2010 Edition.
- Venardos K, Harrison G, Headrick J, et al. (2004): Effects of dietary selenium on glutathione peroxidase and thioredoxin reductase activity and recovery from cardiac ischemia-reperfusion. J Trace Elem Med Biol, 18, 81-88.
- Verma PK, Singh Y, Raina R, et al. (2012): Stress biomarkers in Vanaraja chicken maintained under various rearing systems. J Advan Vet Res, 2, 5-8.
- Wang XJ, Li Y, Song QQ, et al. (2013): Corticosterone regulation of ovarian follicular development is dependent on the energy status of laying hens. J Lipid Res, 54, 1860-
There are 50 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA26JY56UU |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2018 |
| Published in Issue | Year 2018 |