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Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin

Year 2015, , 45 - 50, 01.03.2015

Gökhan Nur Sevda Eliş Yıldız Mümtaz Nazlı Mahmut Sözmen

https://doi.org/10.1501/Vetfak_0000002656
Cited By: 1

Abstract

In this study, the effect of capsaicin on the immunohistochemical distribution of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) were examined in liver of the rat at the pubertal age.Thirty rats (Sprague-Dawley) aging 50 days old were used in this study. Rats were divided into three groups; experimental, sham and control group. Experimental group (n = 10) rats, was injected 1 mg / kg doses of capsaicin (10% ethanol, 1% tween, containing 80% distilled water mixture) subcutaneously every day for a period of one week. Sham group (n = 10) rats, were subcutaneously injected with a mixture containing 10% ethanol and 1% tween within 80% distilled water. The control group made no application. A week after the last injection, rats of three groups body was weighed and euthanized with ether anesthesia and liver tissues were taken. Body weights compared within the groups.The tissue localization of COX-1 and COX-2 was analysed by immunohistochemical method.Streptavidin-biotin-peroxidase complex technique was applied.COX-1 immunoreactivity experimental, sham and control groups were observed in centralis vein and the environment Kiernan centrilobuler region. Immunoreactivity was found in similar concentrations in all three groups. COX-2 is a experimental, sham and control groups were observed in centralis vein and Kiernan centrilobuler region in the portal area

Keywords

Capsaicin COX-1 COX-2 immunohistochemistry liver

References

  • Bhandari P, Bateman AC, Mehta RL, Patel P (2005): Mucosal expression of cyclooxygenase isoforms 1 and 2 is increased with worsening damage to the gastric mucosa. Histopathology, 46: 280-6.
  • Birnbaum Y, Ye Y, Rosanio S, Tavackoli S, Hu ZY, Schwarz ER, Uretsky BF (2005): Prostaglandins mediate the cardioprotective effects of atorvastatin against ischemia-reperfusion injury. Cardiovasc. Res., 65: 345-55.
  • Cao M, Dong L, Lu X, Luo J (2008): Expression of cyclooxygenase-2 and its pathogenic effects in nonalcoholic fatty liver disease. Journal of Nanjing Medical University (JNMU), 22: 111-116.
  • Caterina MJ, Julius D (2001): The vanilloid Receptor: A Moleculer Gateway to the pain pathway. Annual Review of Neuroscience, 24: 487-517.
  • Dubois RN, Abramson SB, Crofford L (1998): Cyclooxygenase in biology and disease. The FASEB Journal, 12: 1063-73.
  • Heitmeier MR, Kelly CB, Ensor NJ, Gibson KA (2004): Role of cyclooxygenase-2 in cytokine-induced ß-cell dysfunction and damage by isolated rat and human islets. JBC Papers in Press, 279: 53145-53151.
  • Inoue H, Tanabe T, Umesono K (2000): Feedback control of cyclooxygenase-2 expression through PPARgamma. J. Biol. Chem., 275: 28028-32.
  • Jancso G, Such G (1983): Effects of capsaicin applied perineurally to the vagus nerve on cardiovascular and respiratory functions in the cat. The Journal of Physiology, 341: 359-370.
  • Kawada T, Hagihara K, Iwai K (1986): Effects of capsaicin on lipid metabolism in rats fed high fat diet. J Nutr., 116, 1272-1278.
  • Kawada T, Sakabe S, Watanabe T, Yamamoto M, Iwai K (1988): Some pungent principles of spices cause the adrenal medulla to secrete catecholamine in anesthetized rats. Proc Soc Exp Biol Med., 188, 229-233.
  • Keller SA, Paxian M, Lee SM, Clemens MG, Huynh T (2005): Kupffer cell ablation attenuates cyclooxygenase-2 Expression after Trauma and Sepsis. Journal of Surgical Research, 124, 126-133.
  • Kress M, Gutimann C, Averbeck B, Reeh PW (1999): Calcitonin gene related peptid and prostaglandin E2 but not substance P release induced by antidromic nevre stimulation from rat skin in vitro. Neuroscience, 89: 303-10.
  • Lembeck F (1987): Columbus, capsicum and capsaicin: Past, present and future. Acta Physiology of Hungary, 69: 265-73.
  • Liang YC, Huang YT, Tsai SH, Lin-Shiau SY, Chen CF, Lin JK (1999): Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis, 20: 1945-52.
  • Meinyk A, Himms-Hagen J (1995): Resistance to aging- associated obesity in capsaicin-desensitized rats one year after treatment. Obesity Research, 3: 337-44.
  • Monsereenusorn Y (1983): Subchronic toxicity studies of capsaicin and capsicum in rats. Research Communications in Chemical Pathology and Pharmacology, 41: 95-110.
  • Oh W, Ohta F (2003): Dose-dependent effect of capsaicin on endurance capacity in rats. British Journal of Nutrition, 90, 515-20.
  • Park ES, Jo S, Yi SJ, Kim JS, Lee HS, Lee IS, Seo KM, Sung JK, Lee I, Yoon YS (2004): Effect of capsaicin on cholecystokinin and neuropeptide Y ekspressions in the brain of high-fat diet fed rats. Journal of Veterinary Medical Science, 66: 107-114.
  • Sambaiah K, Satyanarayana MN (1982): Influence of red pepper and capsaicin on body composition and lipogenesis in rats. Journal of Bioscience, 4: 425-430.
  • Seidal T, Balaton AJ, Battifora H (2001): Interpretation and quantification of immunostains. Am J Surg Pathol, 25:1204–1207.
  • Shu S, G Ju, L Fan (1988): The glucose oxidase-dab- nickel in peroxidase histochemistry of the nervous system. Neuroscience Lett, 85: 169–171.
  • Smith WL, DeWitt DL, Garavito RM (2000): Cyclooxygenases: structural, cellular and molecular biology. Annu. Rev. Biochem., 69: 145-182.
  • Smith WL, Garavito RM, DeWitt DL (1996): Prostaglandin Endoperoxide H Sand-2. The Journal of Biological Chemistry, 271: 33157-33160.
  • Srinivasan MR, Satyanarayana MN (1987): Influence of capsaicin, curcumin and ferulic acid in rats fed high fat diets. Journal of Bioscience, 12: 143-152.
  • Szallasi A, Blumberg P.M (1999): Vanilliod (capsaicin) receptors and mechanisms. Pharmacological Reviews, 51: 159-211.
  • Şahin AF (2008): Kalın Barsak Adenokarsinomları ile adenomlarında COX-2, Beta-cetanin ve p53 İmmuno- reaktivitelerinin Değerlendirilmesi. Düzce Üniversitesi Sağlık Bilimleri Enstitüsü, Patoloji A.D., Uzmanlık Tezi, Düzce.
  • Takahata K, Chen X, Monobe K, Tada M (1999): Growth inhibition of capsaicin on hela cells is not mediated by intracellular calcium mobilization. Life Sciences, 64: 165, 71.
  • Takeeda M, Hayashi Y, Yamato M, Murakami M, Takeuchi K (2004): Roles of endogenous prostaglandins and cyclooxygenase izoenzymes in mucosal defense of inflamed rat stomach. J. Physiol. Pharmacol., 55: 193-205.
  • Teng CH, Kang JY, Wee A, Lee KO (1998): Protective action of capsaicin and chilli on haemorrhagic shock- induced gastric mucosal injury in the rat. Journal of Gastroenterology and Hepatology, 13: 1007-1014.
  • Tominaga M, Julius D (2000): Capsaicin receptor in the pain Pathway. The Japanase Journal of Pharmacology, 83: 20-24.
  • Turini ME, Dubois RN (2002): Cyclooxygenase-2: A Therapeutic Target. Annu. Rev. Med., 53: 35-57.
  • Tütüncü Ş, Özfiliz N (2009): Gelişme Sürecinde Capsaicin Uygulanmış Sıçan Ovaryumlarında Vanilloid Reseptör 1’in İmmunohistokimyasal Ekspresyonu. Uludağ Üniversitesi Doktora Tezi, Bursa.
  • Vane JR, Bakhle YS, Botting RM (1998): Cyclooxygenase. Pharmacol. Toxicol., 38: 97-120.
  • Yar AS (2008): Streptozotosin ile oluşturulmuş diyabetli sıçanlarda resveratrol kullanımı sonucunda siklooksijenaz- 1 (COX-1) ve siklooksijenaz-2 (COX-2) genlerinin ekspre- sonlarının araştırılması. Gazi Üniversitesi Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik A.D., Yüksek Lisans Tezi, Ankara.
  • Zhu QY (1989): Analysis of blood vessel invasion by cells of thyroid follicular carcinoma using image processing combined with immunohistochemistry. Zhonghua Yi Xue Za Zhi, 69:573–575. Geliş tarihi: 22.11.2013/ Kabul tarihi: 12.06.2014

Puberte döneminde capsaicin uygulanan sıçanların karaciğerinde COX-1 ve COX-2' nin immunohistokimyasal lokalizasyonu

Year 2015, , 45 - 50, 01.03.2015

Gökhan Nur Sevda Eliş Yıldız Mümtaz Nazlı Mahmut Sözmen

https://doi.org/10.1501/Vetfak_0000002656
Cited By: 1

Abstract

Çalışmamızda, puberte döneminde capsaicin uygulanan sıçanların karaciğerinde siklooksijenaz-1 (COX-1) ve siklooksijenaz-2 (COX-2)’nin immunohistokimyasal lokalizasyonu incelendi. Çalışmada 50 günlük 30 erkek sıçan (Sprague-Dawley ırkı) kullanıldı. Sıçanlar deneme, sham ve kontrol olmak üzere 3 gruba ayrıldı. Deneme grubundaki (n=10) sıçanlara subkutan yolla her gün 1 mg/kg dozda capsaicin, % 10 ethanol, % 1 tween, % 80 distile su içeren karışım bir hafta süre ile enjekte edildi. Sham grubunu (n=10) oluşturan sıçanlara da aynı deneme grubundaki sıçanlarda olduğu gibi subkutan yolla CAP yerine sadece % 10 ethanol, % 1 tween, % 80 distile su içeren karışım subkutan olarak yapıldı. Kontrol grubuna (n=10) ise herhangi bir uygulama yapılmadı. Bir haftanın sonunda her üç gruptaki hayvanların vücut ağırlıkları tartıldıktan sonra eter ile ötenazi edildi ve karaciğer dokuları alındı. Grupların canlı ağırlıkları karşılaştırıldı. COX-1 ve COX-2 lokalizasyonu, immunohistokimyasal yöntemle tespit edildi. Streptavidin-Biotin-Peroksidaz Kompleks tekniği uygulandı. COX-1’in immunoreaktivitesi deneme, sham ve kontrol gruplarında vena sentralis ve Kiernan aralığı çevresindeki hepatositlerin sitoplazmasında gözlendi. Her üç grupta da immunoreaktivite birbirine benzer yoğunlukta bulundu. COX-2 ise deneme, sham ve kontrol gruplarında vena sentralisler etrafında ve Kiernan aralığına kadar olan portal alanda gözlemlendi

Keywords

Capsaicin COX-1 COX-2 immunohistokimya karaciğer

References

  • Bhandari P, Bateman AC, Mehta RL, Patel P (2005): Mucosal expression of cyclooxygenase isoforms 1 and 2 is increased with worsening damage to the gastric mucosa. Histopathology, 46: 280-6.
  • Birnbaum Y, Ye Y, Rosanio S, Tavackoli S, Hu ZY, Schwarz ER, Uretsky BF (2005): Prostaglandins mediate the cardioprotective effects of atorvastatin against ischemia-reperfusion injury. Cardiovasc. Res., 65: 345-55.
  • Cao M, Dong L, Lu X, Luo J (2008): Expression of cyclooxygenase-2 and its pathogenic effects in nonalcoholic fatty liver disease. Journal of Nanjing Medical University (JNMU), 22: 111-116.
  • Caterina MJ, Julius D (2001): The vanilloid Receptor: A Moleculer Gateway to the pain pathway. Annual Review of Neuroscience, 24: 487-517.
  • Dubois RN, Abramson SB, Crofford L (1998): Cyclooxygenase in biology and disease. The FASEB Journal, 12: 1063-73.
  • Heitmeier MR, Kelly CB, Ensor NJ, Gibson KA (2004): Role of cyclooxygenase-2 in cytokine-induced ß-cell dysfunction and damage by isolated rat and human islets. JBC Papers in Press, 279: 53145-53151.
  • Inoue H, Tanabe T, Umesono K (2000): Feedback control of cyclooxygenase-2 expression through PPARgamma. J. Biol. Chem., 275: 28028-32.
  • Jancso G, Such G (1983): Effects of capsaicin applied perineurally to the vagus nerve on cardiovascular and respiratory functions in the cat. The Journal of Physiology, 341: 359-370.
  • Kawada T, Hagihara K, Iwai K (1986): Effects of capsaicin on lipid metabolism in rats fed high fat diet. J Nutr., 116, 1272-1278.
  • Kawada T, Sakabe S, Watanabe T, Yamamoto M, Iwai K (1988): Some pungent principles of spices cause the adrenal medulla to secrete catecholamine in anesthetized rats. Proc Soc Exp Biol Med., 188, 229-233.
  • Keller SA, Paxian M, Lee SM, Clemens MG, Huynh T (2005): Kupffer cell ablation attenuates cyclooxygenase-2 Expression after Trauma and Sepsis. Journal of Surgical Research, 124, 126-133.
  • Kress M, Gutimann C, Averbeck B, Reeh PW (1999): Calcitonin gene related peptid and prostaglandin E2 but not substance P release induced by antidromic nevre stimulation from rat skin in vitro. Neuroscience, 89: 303-10.
  • Lembeck F (1987): Columbus, capsicum and capsaicin: Past, present and future. Acta Physiology of Hungary, 69: 265-73.
  • Liang YC, Huang YT, Tsai SH, Lin-Shiau SY, Chen CF, Lin JK (1999): Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis, 20: 1945-52.
  • Meinyk A, Himms-Hagen J (1995): Resistance to aging- associated obesity in capsaicin-desensitized rats one year after treatment. Obesity Research, 3: 337-44.
  • Monsereenusorn Y (1983): Subchronic toxicity studies of capsaicin and capsicum in rats. Research Communications in Chemical Pathology and Pharmacology, 41: 95-110.
  • Oh W, Ohta F (2003): Dose-dependent effect of capsaicin on endurance capacity in rats. British Journal of Nutrition, 90, 515-20.
  • Park ES, Jo S, Yi SJ, Kim JS, Lee HS, Lee IS, Seo KM, Sung JK, Lee I, Yoon YS (2004): Effect of capsaicin on cholecystokinin and neuropeptide Y ekspressions in the brain of high-fat diet fed rats. Journal of Veterinary Medical Science, 66: 107-114.
  • Sambaiah K, Satyanarayana MN (1982): Influence of red pepper and capsaicin on body composition and lipogenesis in rats. Journal of Bioscience, 4: 425-430.
  • Seidal T, Balaton AJ, Battifora H (2001): Interpretation and quantification of immunostains. Am J Surg Pathol, 25:1204–1207.
  • Shu S, G Ju, L Fan (1988): The glucose oxidase-dab- nickel in peroxidase histochemistry of the nervous system. Neuroscience Lett, 85: 169–171.
  • Smith WL, DeWitt DL, Garavito RM (2000): Cyclooxygenases: structural, cellular and molecular biology. Annu. Rev. Biochem., 69: 145-182.
  • Smith WL, Garavito RM, DeWitt DL (1996): Prostaglandin Endoperoxide H Sand-2. The Journal of Biological Chemistry, 271: 33157-33160.
  • Srinivasan MR, Satyanarayana MN (1987): Influence of capsaicin, curcumin and ferulic acid in rats fed high fat diets. Journal of Bioscience, 12: 143-152.
  • Szallasi A, Blumberg P.M (1999): Vanilliod (capsaicin) receptors and mechanisms. Pharmacological Reviews, 51: 159-211.
  • Şahin AF (2008): Kalın Barsak Adenokarsinomları ile adenomlarında COX-2, Beta-cetanin ve p53 İmmuno- reaktivitelerinin Değerlendirilmesi. Düzce Üniversitesi Sağlık Bilimleri Enstitüsü, Patoloji A.D., Uzmanlık Tezi, Düzce.
  • Takahata K, Chen X, Monobe K, Tada M (1999): Growth inhibition of capsaicin on hela cells is not mediated by intracellular calcium mobilization. Life Sciences, 64: 165, 71.
  • Takeeda M, Hayashi Y, Yamato M, Murakami M, Takeuchi K (2004): Roles of endogenous prostaglandins and cyclooxygenase izoenzymes in mucosal defense of inflamed rat stomach. J. Physiol. Pharmacol., 55: 193-205.
  • Teng CH, Kang JY, Wee A, Lee KO (1998): Protective action of capsaicin and chilli on haemorrhagic shock- induced gastric mucosal injury in the rat. Journal of Gastroenterology and Hepatology, 13: 1007-1014.
  • Tominaga M, Julius D (2000): Capsaicin receptor in the pain Pathway. The Japanase Journal of Pharmacology, 83: 20-24.
  • Turini ME, Dubois RN (2002): Cyclooxygenase-2: A Therapeutic Target. Annu. Rev. Med., 53: 35-57.
  • Tütüncü Ş, Özfiliz N (2009): Gelişme Sürecinde Capsaicin Uygulanmış Sıçan Ovaryumlarında Vanilloid Reseptör 1’in İmmunohistokimyasal Ekspresyonu. Uludağ Üniversitesi Doktora Tezi, Bursa.
  • Vane JR, Bakhle YS, Botting RM (1998): Cyclooxygenase. Pharmacol. Toxicol., 38: 97-120.
  • Yar AS (2008): Streptozotosin ile oluşturulmuş diyabetli sıçanlarda resveratrol kullanımı sonucunda siklooksijenaz- 1 (COX-1) ve siklooksijenaz-2 (COX-2) genlerinin ekspre- sonlarının araştırılması. Gazi Üniversitesi Sağlık Bilimleri Enstitüsü, Tıbbi Biyoloji ve Genetik A.D., Yüksek Lisans Tezi, Ankara.
  • Zhu QY (1989): Analysis of blood vessel invasion by cells of thyroid follicular carcinoma using image processing combined with immunohistochemistry. Zhonghua Yi Xue Za Zhi, 69:573–575. Geliş tarihi: 22.11.2013/ Kabul tarihi: 12.06.2014
There are 35 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA25ZC99VU
Journal Section Research Article
Authors

Gökhan Nur

Sevda Eliş Yıldız

Mümtaz Nazlı

Mahmut Sözmen

Publication Date March 1, 2015
Published in Issue Year 2015

Cite

APA Nur, G., Eliş Yıldız, S., Nazlı, M., Sözmen, M. (2015). Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 62(1), 45-50. https://doi.org/10.1501/Vetfak_0000002656
AMA Nur G, Eliş Yıldız S, Nazlı M, Sözmen M. Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin. Ankara Univ Vet Fak Derg. March 2015;62(1):45-50. doi:10.1501/Vetfak_0000002656
Chicago Nur, Gökhan, Sevda Eliş Yıldız, Mümtaz Nazlı, and Mahmut Sözmen. “Immunohistochemical Distribution of COX-1 and COX-2 in the Liver to During the Puberty Period Ofrats Applied With Capsaicin”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 62, no. 1 (March 2015): 45-50. https://doi.org/10.1501/Vetfak_0000002656.
EndNote Nur G, Eliş Yıldız S, Nazlı M, Sözmen M (March 1, 2015) Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin. Ankara Üniversitesi Veteriner Fakültesi Dergisi 62 1 45–50.
IEEE G. Nur, S. Eliş Yıldız, M. Nazlı, and M. Sözmen, “Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin”, Ankara Univ Vet Fak Derg, vol. 62, no. 1, pp. 45–50, 2015, doi: 10.1501/Vetfak_0000002656.
ISNAD Nur, Gökhan et al. “Immunohistochemical Distribution of COX-1 and COX-2 in the Liver to During the Puberty Period Ofrats Applied With Capsaicin”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 62/1 (March 2015), 45-50. https://doi.org/10.1501/Vetfak_0000002656.
JAMA Nur G, Eliş Yıldız S, Nazlı M, Sözmen M. Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin. Ankara Univ Vet Fak Derg. 2015;62:45–50.
MLA Nur, Gökhan et al. “Immunohistochemical Distribution of COX-1 and COX-2 in the Liver to During the Puberty Period Ofrats Applied With Capsaicin”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 62, no. 1, 2015, pp. 45-50, doi:10.1501/Vetfak_0000002656.
Vancouver Nur G, Eliş Yıldız S, Nazlı M, Sözmen M. Immunohistochemical distribution of COX-1 and COX-2 in the liver to during the puberty period ofrats applied with capsaicin. Ankara Univ Vet Fak Derg. 2015;62(1):45-50.

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https://doi.org/10.18016/ksutarimdoga.vi.1196713