Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR

Alper Karagöz; Bekir Çelebi; Hülya Şimşek; Müge Taner; Selçuk Kılıç; Rıza Durmaz; Mustafa Ertek

doi:10.1501/Vetfak_0000002590

Letter to Editor

Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR

Year 2013, , 235 - 239, 01.12.2013

Alper Karagöz Bekir Çelebi Hülya Şimşek Müge Taner Selçuk Kılıç Rıza Durmaz Mustafa Ertek

https://doi.org/10.1501/Vetfak_0000002590

Cited By: 7

Abstract

The species within the genus Bartonella are intracellular bacteria causing long-lasting bacteremia in humans and animals. Eleven Bartonella species isolated from animals have zoonotic characteristics. The four of zoonotic Bartonella species have been reported in rodents. In this study, Bartonella spp. in 42 field mice, (Microtus socialis), captured from one spot in Central Anatolia Region were investigated by culture and molecular methods. The positivity of Bartonella was 16.6% (7/42) by culture, and 57.1% (24/42) by PCR (Polymerase Chain Reaction) from liver tissues of field mice. The citrate synthesis gene regions (gltA) of the isolated seven strains were identified by DNA sequencing analysis. It was determined that five isolates were Bartonella taylorii, and two isolates were Bartonella grahamii which are zoonotic. This study is the first report for the presence of Bartonella spp. in rodents in Turkey

Keywords

Bartonella, Microtus socialis, PCR

References

Avidor, B., M. Graidy, G. Efrat, C. Leibowitz, G. Shapira, A.Schattner, O. Zimhony, and M. Giladi, (2004): Bartonella koehlerae, a new cat-associated agent of culturenegative human endocarditis. J. Clin. Microbiol. 42, 3462–3468.
Bermond D, Heller R, Barrat F, Delacour G, Dehio C, Alliot A, Monteil H, Chomel B, Boulouis HJ, Piémont Y. (2000): Bartonella birtlesii sp. nov., isolated from small mammals (Apodemus spp.). Int J Syst Evol Microbiol. 6: 1973-9.
Birtles RJ, Harrison TG, Saunders NA, Molyneux DH. (1995): Proposals to unify the genera Grahamella and Bartonella, with descriptions of Bartonella talpae comb. nov., Bartonella peromysci comb. nov., and three new species, Bartonella grahamii sp. nov., Bartonella taylorii sp. nov., and Bartonella doshiae sp. nov. Int J Syst Bacteriol. 45:1-8.
Birtles RJ, Harrison TG, Molyneux DH. (1994): Grahamella in small woodland mammals in the U.K.: isolation, prevalence and host specificity. Ann Trop Med Parasitol. 88:317-27.
Boulouis HJ, Chang CC, Henn JB, Kasten RW, Chomel BB. (2005): Factors associated with the rapid emergence of zoonotic Bartonella infections. Vet Res. 36:383-410.
Çelebi B. (2008): Bartonella henselae and its infections. Mikrobiyol Bul. 42:163-75.
Celebi B, Kilic S, Aydin N, Tarhan G, Carhan A, Babur C. (2009): Investigation of Bartonella henselae in cats in Ankara, Turkey. Zoonoses Public Health. 56:169- 75.
Celebi B, Carhan A, Kilic S, Babur C. (2010): Detection and genetic diversity of Bartonella vinsonii subsp. berkhoffii strains isolated from dogs in Ankara, Turkey. J Vet Med Sci. 72:969-73.
Ankara Üniv Vet Fak Derg, 60, 2013
Celebi B, Taylan Ozkan A, Kilic S, Akca A, Koenhemsi L, Pasa S, Yildiz K, Mamak N, Guzel M. (2010): Seroprevalence of Bartonella vinsonii subsp. berkhoffii in urban and rural dogs in Turkey. J Vet Med Sci. 72:1491-4.
Çelebi B, Yalçın E, Babür C. (2010): Fever of unknown origin and detection of Bartonella henselae IgG seropositivity: a case report. Mikrobiyol Bul. 44:489-94.
Chomel, B. B., H. J. Boulouis, and E. B. Breitschwerdt,(2004): Cat scratch disease and other zoonotic Bartonella infections. J. Am. Vet. Med. Assoc. 224, 1270–1279.
Engbaek K, Lawson PA.(2004): Identification of Bartonella species in rodents, shrews and cats in Denmark: detection of two B. henselae variants, one in cats and the other in the long-tailed field mouse. APMIS. 112:336-41.
Gil H, García-Esteban C, Barandika JF, Peig J, Toledo A, Escudero R, Jado I, Rodríguez-Vargas M, García- Amil C, Lobo B, Roales P, Rodríguez-Moreno I, Olmeda AS, García-Pérez AL, Anda. P. (2010): Variability of Bartonella genotypes among small mammals in Spain. Appl Environ Microbiol. 76:8062-70.
Guzel M, Celebi B, Yalcin E, Koenhemsi L, Mamak N, Pasa S, Aslan O. (2011): A serological investigation of Bartonella henselae infection in cats in Turkey. J Vet Med Sci. 73:1513-6.
Holmberg M, Mills JN, McGill S, Benjamin G, Ellis BA.(2003): Bartonella infection in sylvatic small mammals of central Sweden. Epidemiol Infect. 130:149-57.
Iralu J, Bai Y, Crook L, Tempest B, Simpson G, Mckenzie T, Koster F. (2006): Rodent-associated Bartonella febrile illness, Southwestern United States. Emerg Infect Dis. 12:1081-6.
Kerkhoff FT, Bergmans AM, van Der Zee A, Rothova A. (1999): Demonstration of Bartonella grahamii DNA in ocular fluids of a patient with neuroretinitis. J Clin Microbiol. 37: 4034-8.
Knap N, Duh D, Birtles R, Trilar T, Petrovec M, Avsic- Zupanc T. (2007): Molecular detection of Bartonella species infecting rodents in Slovenia. FEMS Immunol Med Microbiol. 50: 45-50.
Maggi RG, Chomel B, Hegarty BC, Henn J, Breitschwerdt EB. (2006): A Bartonella vinsonii berkhoffii typing scheme based upon 16S-23S ITS and Pap31 sequences from dog, coyote, gray fox, and human isolates.Mol Cell Probes. 20:128-34.
Norman AF, Regnery R, Jameson P, Greene C, Krause DC. (1995): Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J Clin Microbiol.33:1797-803.
Reis C, Cote M, Le Rhun D, Lecuelle B, Levin ML, Vayssier-Taussat M, Bonnet SI. (2011): Vector competence of the tick Ixodes ricinus for transmission of Bartonella birtlesii. PLoS Negl Trop Dis.5:1186.
Serratrice J, Rolain JM, Granel B, Ene N, Conrath J, Avierinos JF, Disdier P, Raoult D, Weiller PJ.(2003): Bilateral retinal artery branch occlusions revealing Bartonella grahamii infection. Rev Med Interne. 24:629- 30.
Tea A, Alexiou-Daniel S, Papoutsi A, Papa A, Antoniadis A. (2004): Bartonella species isolated from rodents, Greece. Emerg Infect Dis. 10:963-4
Welch DF, Carroll KC, Hofmeister EK, Persing DH, Robison DA, Steigerwalt AG, Brenner DJ. (1999): Isolation of a new subspecies, Bartonella vinsonii subsp. arupensis, from a cattle rancher: identity with isolates found in conjunction with Borrelia burgdorferi and Babesia microti among naturally infected mice. J Clin Microbiol.37:2598-601.
Welc-Faleciak R, Paziewska A, Bajer A, Behnke JM, Siński E. (2008): Bartonella spp. infection in rodents from different habitats in the Mazury Lake District, Northeast Poland. Vector Borne Zoonotic Dis. 8:467-74.
Yılmaz C, Ergin C, Kaleli I. (2009):Investigation of Bartonella henselae seroprevalence and related risk factors in blood donors admitted to Pamukkale University Blood Center Mikrobiyol Bul. 43:391-401.

Tarla farelerinde (Microtus socialis) Bartonella Spp. varlığının kültür ve PCR yöntemleriylebelirlenmesi

Year 2013, , 235 - 239, 01.12.2013

Alper Karagöz Bekir Çelebi Hülya Şimşek Müge Taner Selçuk Kılıç Rıza Durmaz Mustafa Ertek

https://doi.org/10.1501/Vetfak_0000002590

Cited By: 7

Abstract

Bartonella genusundaki türler insanlarda ve hayvanlarda uzun süreli bakteriyemiye neden olan hücre içi bakterilerdir. Hayvanlardan izole edilen onbir Bartonella türü zoonoz karakterdedir. Zoonoz Bartonella türlerinden dördü rodentlerde bildirilmiştir. Bu çalışmada Orta Anadolu Bölgesinde bir odaktan yakalanan 42 Microtus socialis türü tarla faresinde kültür ve moleküler yöntemlerle Bartonella spp. araştırıldı. Tarla farelerinde, karaciğer dokusundan kültür ile % 16.6 (7/42), PCR (Polimeraz Zincir Reaksiyonu) ile % 57,1 (24/42) Bartonella pozitifliği tespit edildi. Kültürde izole edilen yedi izolatın, sitrat sentez gen bölgesi (gltA) DNA dizi analizi ile tür tanımlamaları yapıldı. İzolatlardan beşi Bartonella taylorii, ikisi zoonotik bir tür olan Bartonella grahamii olarak belirlendi. Bu çalışma ile ülkemizde rodentlerde ilk defa Bartonella türlerinin varlığı bildirilmektedir

Keywords

Bartonella, Microtus socialis, PCR

References

Avidor, B., M. Graidy, G. Efrat, C. Leibowitz, G. Shapira, A.Schattner, O. Zimhony, and M. Giladi, (2004): Bartonella koehlerae, a new cat-associated agent of culturenegative human endocarditis. J. Clin. Microbiol. 42, 3462–3468.
Bermond D, Heller R, Barrat F, Delacour G, Dehio C, Alliot A, Monteil H, Chomel B, Boulouis HJ, Piémont Y. (2000): Bartonella birtlesii sp. nov., isolated from small mammals (Apodemus spp.). Int J Syst Evol Microbiol. 6: 1973-9.
Birtles RJ, Harrison TG, Saunders NA, Molyneux DH. (1995): Proposals to unify the genera Grahamella and Bartonella, with descriptions of Bartonella talpae comb. nov., Bartonella peromysci comb. nov., and three new species, Bartonella grahamii sp. nov., Bartonella taylorii sp. nov., and Bartonella doshiae sp. nov. Int J Syst Bacteriol. 45:1-8.
Birtles RJ, Harrison TG, Molyneux DH. (1994): Grahamella in small woodland mammals in the U.K.: isolation, prevalence and host specificity. Ann Trop Med Parasitol. 88:317-27.
Boulouis HJ, Chang CC, Henn JB, Kasten RW, Chomel BB. (2005): Factors associated with the rapid emergence of zoonotic Bartonella infections. Vet Res. 36:383-410.
Çelebi B. (2008): Bartonella henselae and its infections. Mikrobiyol Bul. 42:163-75.
Celebi B, Kilic S, Aydin N, Tarhan G, Carhan A, Babur C. (2009): Investigation of Bartonella henselae in cats in Ankara, Turkey. Zoonoses Public Health. 56:169- 75.
Celebi B, Carhan A, Kilic S, Babur C. (2010): Detection and genetic diversity of Bartonella vinsonii subsp. berkhoffii strains isolated from dogs in Ankara, Turkey. J Vet Med Sci. 72:969-73.
Ankara Üniv Vet Fak Derg, 60, 2013
Celebi B, Taylan Ozkan A, Kilic S, Akca A, Koenhemsi L, Pasa S, Yildiz K, Mamak N, Guzel M. (2010): Seroprevalence of Bartonella vinsonii subsp. berkhoffii in urban and rural dogs in Turkey. J Vet Med Sci. 72:1491-4.
Çelebi B, Yalçın E, Babür C. (2010): Fever of unknown origin and detection of Bartonella henselae IgG seropositivity: a case report. Mikrobiyol Bul. 44:489-94.
Chomel, B. B., H. J. Boulouis, and E. B. Breitschwerdt,(2004): Cat scratch disease and other zoonotic Bartonella infections. J. Am. Vet. Med. Assoc. 224, 1270–1279.
Engbaek K, Lawson PA.(2004): Identification of Bartonella species in rodents, shrews and cats in Denmark: detection of two B. henselae variants, one in cats and the other in the long-tailed field mouse. APMIS. 112:336-41.
Gil H, García-Esteban C, Barandika JF, Peig J, Toledo A, Escudero R, Jado I, Rodríguez-Vargas M, García- Amil C, Lobo B, Roales P, Rodríguez-Moreno I, Olmeda AS, García-Pérez AL, Anda. P. (2010): Variability of Bartonella genotypes among small mammals in Spain. Appl Environ Microbiol. 76:8062-70.
Guzel M, Celebi B, Yalcin E, Koenhemsi L, Mamak N, Pasa S, Aslan O. (2011): A serological investigation of Bartonella henselae infection in cats in Turkey. J Vet Med Sci. 73:1513-6.
Holmberg M, Mills JN, McGill S, Benjamin G, Ellis BA.(2003): Bartonella infection in sylvatic small mammals of central Sweden. Epidemiol Infect. 130:149-57.
Iralu J, Bai Y, Crook L, Tempest B, Simpson G, Mckenzie T, Koster F. (2006): Rodent-associated Bartonella febrile illness, Southwestern United States. Emerg Infect Dis. 12:1081-6.
Kerkhoff FT, Bergmans AM, van Der Zee A, Rothova A. (1999): Demonstration of Bartonella grahamii DNA in ocular fluids of a patient with neuroretinitis. J Clin Microbiol. 37: 4034-8.
Knap N, Duh D, Birtles R, Trilar T, Petrovec M, Avsic- Zupanc T. (2007): Molecular detection of Bartonella species infecting rodents in Slovenia. FEMS Immunol Med Microbiol. 50: 45-50.
Maggi RG, Chomel B, Hegarty BC, Henn J, Breitschwerdt EB. (2006): A Bartonella vinsonii berkhoffii typing scheme based upon 16S-23S ITS and Pap31 sequences from dog, coyote, gray fox, and human isolates.Mol Cell Probes. 20:128-34.
Norman AF, Regnery R, Jameson P, Greene C, Krause DC. (1995): Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J Clin Microbiol.33:1797-803.
Reis C, Cote M, Le Rhun D, Lecuelle B, Levin ML, Vayssier-Taussat M, Bonnet SI. (2011): Vector competence of the tick Ixodes ricinus for transmission of Bartonella birtlesii. PLoS Negl Trop Dis.5:1186.
Serratrice J, Rolain JM, Granel B, Ene N, Conrath J, Avierinos JF, Disdier P, Raoult D, Weiller PJ.(2003): Bilateral retinal artery branch occlusions revealing Bartonella grahamii infection. Rev Med Interne. 24:629- 30.
Tea A, Alexiou-Daniel S, Papoutsi A, Papa A, Antoniadis A. (2004): Bartonella species isolated from rodents, Greece. Emerg Infect Dis. 10:963-4
Welch DF, Carroll KC, Hofmeister EK, Persing DH, Robison DA, Steigerwalt AG, Brenner DJ. (1999): Isolation of a new subspecies, Bartonella vinsonii subsp. arupensis, from a cattle rancher: identity with isolates found in conjunction with Borrelia burgdorferi and Babesia microti among naturally infected mice. J Clin Microbiol.37:2598-601.
Welc-Faleciak R, Paziewska A, Bajer A, Behnke JM, Siński E. (2008): Bartonella spp. infection in rodents from different habitats in the Mazury Lake District, Northeast Poland. Vector Borne Zoonotic Dis. 8:467-74.
Yılmaz C, Ergin C, Kaleli I. (2009):Investigation of Bartonella henselae seroprevalence and related risk factors in blood donors admitted to Pamukkale University Blood Center Mikrobiyol Bul. 43:391-401.

There are 27 citations in total.

Details

Primary Language	English
Subjects	Veterinary Surgery
Other ID	JA27UJ45YT
Journal Section	Research Article
Authors	Alper Karagöz Bekir Çelebi Hülya Şimşek Müge Taner Selçuk Kılıç Rıza Durmaz Mustafa Ertek
Publication Date	December 1, 2013
Published in Issue	Year 2013

Cite

APA	Karagöz, A., Çelebi, B., Şimşek, H., Taner, M., et al. (2013). Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 60(4), 235-239. https://doi.org/10.1501/Vetfak_0000002590
AMA	Karagöz A, Çelebi B, Şimşek H, Taner M, Kılıç S, Durmaz R, Ertek M. Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR. Ankara Univ Vet Fak Derg. December 2013;60(4):235-239. doi:10.1501/Vetfak_0000002590
Chicago	Karagöz, Alper, Bekir Çelebi, Hülya Şimşek, Müge Taner, Selçuk Kılıç, Rıza Durmaz, and Mustafa Ertek. “Detection of Bartonella Spp. In Field Mice (Microtus Socialis) by Culture and PCR”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60, no. 4 (December 2013): 235-39. https://doi.org/10.1501/Vetfak_0000002590.
EndNote	Karagöz A, Çelebi B, Şimşek H, Taner M, Kılıç S, Durmaz R, Ertek M (December 1, 2013) Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60 4 235–239.
IEEE	A. Karagöz, B. Çelebi, H. Şimşek, M. Taner, S. Kılıç, R. Durmaz, and M. Ertek, “Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR”, Ankara Univ Vet Fak Derg, vol. 60, no. 4, pp. 235–239, 2013, doi: 10.1501/Vetfak_0000002590.
ISNAD	Karagöz, Alper et al. “Detection of Bartonella Spp. In Field Mice (Microtus Socialis) by Culture and PCR”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60/4 (December 2013), 235-239. https://doi.org/10.1501/Vetfak_0000002590.
JAMA	Karagöz A, Çelebi B, Şimşek H, Taner M, Kılıç S, Durmaz R, Ertek M. Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR. Ankara Univ Vet Fak Derg. 2013;60:235–239.
MLA	Karagöz, Alper et al. “Detection of Bartonella Spp. In Field Mice (Microtus Socialis) by Culture and PCR”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 60, no. 4, 2013, pp. 235-9, doi:10.1501/Vetfak_0000002590.
Vancouver	Karagöz A, Çelebi B, Şimşek H, Taner M, Kılıç S, Durmaz R, Ertek M. Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR. Ankara Univ Vet Fak Derg. 2013;60(4):235-9.