Letter to Editor
Investigation of the levels of serum haptoglobin, oxidative indicators and some biochemicalparameters in calves naturally infected with Toxocara vitulorum
Abstract
The purpose of the study was to determine the levels of serum haptoglobin, oxidative indicators and some biochemical parameters in calves naturally infected with Toxocara vitulorum, and to investigate their diagnostic importance. For this purpose, 10 clinically healthy calves as control and 20 calves with naturally infected with T. vitulorum at 1-3 months of age with different gender total 30 Brown Swiss calves were used. Blood samples were collected from V. jugularis for biochemical analyses. Part of blood samples were stored without any process as a whole blood. The serum was seperated from remaining blood samples. Whole blood reduced glutathione (GSH), serum malondialdehyde (MDA), haptoglobin, albumin, aspartate amino transferase (AST), alkaline phosphatase (ALP), urea, total protein and iron (Fe) levels were determined by colorimetric methods. Levels of serum haptoglobin (P<0.01), AST, ALP and MDA (P<0.05) were determined to increase in blood whereas the levels of albumin, total protein, Fe, GSH (P<0.05) decreased in calves infected with T. vitulorum. In conclusion, the results indicated that the acute phase response was occured in calves infected with T. vitulorum, and as a results of this, the levels of serum haptoglobin synthesis increased, the functions of liver deteriorated and oxidative stress was amplified
References
- Akaike T, Suga M, Maeda H (1998): Free radicals in viral pathogenesis. Moleculer mechanisms involving superoxide and NO. Proc Soc Exp Biol Med, 217, 64-73.
- Arslan MÖ, Sarı B, Taşçı GT ve ark. (2008): Prevalence of Toxocara vitulorum in calves of Erzurum province. Kafkas Univ Vet Med J, 14, 37-40.
- Avcıoğlu H, Balkaya İ (2011): Prevalence of Toxocara vitulorum in calves in Erzurum, Turkey. Kafkas Univ Vet Med J, 17, 345-347.
- Beutler E, Duron O, Kelly BM (1963): Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882-888.
- Cighetti G, Duca L, Bortone L ve ark. (2002): Oxidative status and malondialdehyde in beta-thalassaemia patients. Eur J Clin Invest, 32, 55-60.
- Dede S, Değer Y, Kahraman T ve ark. (2002): Oxidation products of nitric oxide and concentration of antioxidant vitamins in parasitized goats. Acta Vet Brno, 71, 341-345.
- Değer S, Değer Y, Ertekin A ve ark. (2008a): Determination of the status of lipid peroxidation and antioxidants in cattle infected with Dictyocaulus viviparus. Turkiye Parazitol Derg, 32, 234-237.
- Değer Y, Ertekin A, Değer S ve ark. (2008b): Lipid peroxidation and antioxidant potential of sheep liver infected naturally with Distomatosis. Turkiye Parazitol Derg, 32, 23-26.
- Dzik JM, Gołos B, Jagielska E ve ark. (2002): Early response of guinea-pig lungs to Trichinella spiralis infection. Parasite Immunol, 24, 369-379.
- Eckersall PD (2000): Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue Med Vet, 151, 577-584.
- Eckersall PD, Bell R (2010): Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27.
- Eckersall PD, Conner JG (1988): Bovine and canine acute phase proteins. Vet Res Commun, 12, 169-178.
- Enemark HL, Bille-Hansen V, Lind P ve ark. (2003): Pathogenicity of Cryptosporidium parvum-evaluation of an animal infection model. Vet Parasitol, 113, 35-57.
- Ganheim C, Höglund J, Waller KP (2004): Acute phase proteins in response to Dictyocaulus viviparus infection in calves. Acta Vet Scand, 45, 79-86.
- Glass EJ, Craigmile SC, Springbett A ve ark. (2003): The protozoan parasite, Theileria annulata, induces a distinct acute phase protein response in cattle that is associated with pathology. Int J Parasitol, 33, 1409-1418.
- Gruys E, Toussaint MJM, Niewold TA ve ark. (2005): Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci B, 6, 1045-1056.
- Gutteridge JM (1993): Free radicals in disease processes: A compilation of cause and consequence. Free Radic Res Commun, 19, 141-158.
- Hayat CS, Khalid M, Iqbal Z ve ark. (1999): Haematological and biochemical disturbance associated with Toxocara vitulorum infection in buffalo calves. Int J Agri Biol, 4, 247-249.
- Kaneko JJ, Harvey JW, Bruss ML (2008): Clinical Biochemistry of Domestic Animals. Sixth Ed., Academic Press, New York, pp 364-390.
- Katz D, Mazor D, Dvilansky A ve ark. (1996): Effects of radiation on red cell membrane and intracelluler oxidative defense systems. Free Radic Res, 24, 199-204.
- Kolodziejczyk L, Siemieniuk E, Skrzydlewska E (2005): Antioxidant potential of rat liver in experimental infection with Fasciola hepatica. Parasitol Res, 96, 367-372.
- Kozan E, Avcı G, Sevimli Kırcalı F ve ark. (2010): Determine of the antioxidant levels and some biochemical parameters on infected with ascaridiosis and treated dogs. Vet J Ankara Univ, 57, 93-97.
- Lobetti RG, Möhr AJ, Dippenaar T ve ark. (2000): A preliminary study on the serum protein response in canine babesiosis. J S Afr Vet Assoc, 71, 38-42.
- Özcel MA (2013): Veteriner Hekimliğinde Parazit Hastalıkları, First Ed., Meta basım, İzmir, pp 233-237.
- Özkurt G, Gökçen A, Çamkerten İ ve ark. (2012): Erythrocyte SOD, CAT, GPx enzymes activity and MDA level in Kilis goats with naturally-occurred nematodiasis. Harran Univ Vet Med J, 1, 107-110.
- Petersen HH, Nielsen JP, Heegaard PM (2004): Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35, 163-187.
- Radostits OM, Blood DC, Gay CC (1994): Askarid infections. In: Radostits, O.M., Blood, D.C., Gay, C.C., (Eds.), Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses, 8th edition, London, Saunders Company, pp 1556-1558.
- Russell KE, Roussel AJ (2007): Evaluation of the ruminant serum chemistry profile. Vet Clin Food Anim, 23, 403-426.
- Sarma K, Saravanan M, Mondal DB ve ark. (2012): Influence of natural infection of Toxocara vitulorum on markers of oxidative stress in Indian buffalo calves. Indian J Anim Sci, 82, 1142-1145.
- Sezer K, Keskin M (2014): Role of the free oxygen radicals on the pathogenesis of the diseases. Fırat Univ Vet J Health Sci, 28, 49-56.
- Skinner JG, Brown RA, Roberts L (1991): Bovine haptoglobin conditions.Vet Rec, 128, 147-149. clinically defined field
- SPSS (2008): SPSS for windows release 16.0.2. SPSS Inc., Chicago
- Tabakoğlu E, Durgut R (2013): Oxidative stress in veterinary medicine and effects in some important diseases. AVKAE J, 3, 69-75.
- Tothova C, Nagy O, Kovac G (2014): Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med, 59, 163-180.
- Toussaint MJM, van Ederen AM, Gruys E (1995): Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Int, 5, 149-157.
- Ulutas B, Bayramli G, Ulutas PA ve ark. (2005): Serum concentration of some acute phase proteins in naturally occuring canine babesiosis: Preliminary studies. Vet Clin Pathol, 34, 144-147.
- Ulutaş PA, Voyvoda H, Ulutaş B ve ark. (2008): Haptoglobin, concentrations in goats with mixed helminth infection. Turkiye Parazitol Derg, 32, 229-233. and ceruloplasmin
- Urquhart GM, Armour J, Duncan JL ve ark. (1996): Veterinary Parasitology. 2nd Ed., Blackwell Scientific Publications, Oxford, pp 270.
- Van Der Steen L, Pardon B, Sarre C ve ark. (2014): Intestinal obstruction by Toxocara vitulorum in a calf. Vlaams Diergen Tijds, 83, 299-305.
- Yoshioka T, Kawada K, Shimada T ve ark. (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376. Geliş tarihi: 03.02.2016 /Kabul tarihi:18.08.2016
Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesi
Abstract
Bu çalışmanın amacı Toxocara vitulorum ile doğal enfekte buzağılarda serum haptoglobin düzeyi, oksidatif belirteçler ve bazı biyokimyasal parametrelerin düzeylerinin belirlenmesi ve diyagnostik önemlerinin araştırılmasıdır. Bu amaçla, kontrol grubu olarak 10 adet klinik açıdan sağlıklı buzağı ve 1-3 aylık, 20 adet farklı cinsiyette T. vitulorum ile doğal enfekte toplam 30 adet montofon ırkı buzağı kullanıldı. Biyokimyasal analizler için kan örnekleri V. jugularis’ten alındı. Alınan kan örneklerinden bir miktarı tam kan olarak ayrıldıktan sonra geriye kalan kanların serumları elde edildi. Tam kan redükte glutatyon (GSH), serum malondialdehit (MDA), haptoglobin, albümin, aspartat amino transferaz (AST), alkalen fosfataz (ALP), üre, toplam protein ve demir (Fe) düzeyleri kolorimetrik yöntemlerle belirlendi. T. vitulorum ile doğal enfekte buzağılarda serum haptoglobin (P<0.01), AST, ALP ve MDA değerlerinin yükseldiği (P<0.05) albümin, toplam protein, Fe, GSH değerinin ise azaldığı belirlendi (P<0.05). Sonuç olarak, T. vitulorum ile enfekte buzağılarda akut faz yanıt oluştuğu ve bunun sonucu olarak da serum haptoglobin sentezinin arttığı, karaciğerin etkilenip fonksiyonlarının bozulduğu ve oksidatif stres oluştuğu belirlendi
References
- Akaike T, Suga M, Maeda H (1998): Free radicals in viral pathogenesis. Moleculer mechanisms involving superoxide and NO. Proc Soc Exp Biol Med, 217, 64-73.
- Arslan MÖ, Sarı B, Taşçı GT ve ark. (2008): Prevalence of Toxocara vitulorum in calves of Erzurum province. Kafkas Univ Vet Med J, 14, 37-40.
- Avcıoğlu H, Balkaya İ (2011): Prevalence of Toxocara vitulorum in calves in Erzurum, Turkey. Kafkas Univ Vet Med J, 17, 345-347.
- Beutler E, Duron O, Kelly BM (1963): Improved method for the determination of blood glutathione. J Lab Clin Med, 61, 882-888.
- Cighetti G, Duca L, Bortone L ve ark. (2002): Oxidative status and malondialdehyde in beta-thalassaemia patients. Eur J Clin Invest, 32, 55-60.
- Dede S, Değer Y, Kahraman T ve ark. (2002): Oxidation products of nitric oxide and concentration of antioxidant vitamins in parasitized goats. Acta Vet Brno, 71, 341-345.
- Değer S, Değer Y, Ertekin A ve ark. (2008a): Determination of the status of lipid peroxidation and antioxidants in cattle infected with Dictyocaulus viviparus. Turkiye Parazitol Derg, 32, 234-237.
- Değer Y, Ertekin A, Değer S ve ark. (2008b): Lipid peroxidation and antioxidant potential of sheep liver infected naturally with Distomatosis. Turkiye Parazitol Derg, 32, 23-26.
- Dzik JM, Gołos B, Jagielska E ve ark. (2002): Early response of guinea-pig lungs to Trichinella spiralis infection. Parasite Immunol, 24, 369-379.
- Eckersall PD (2000): Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue Med Vet, 151, 577-584.
- Eckersall PD, Bell R (2010): Acute phase proteins: Biomarkers of infection and inflammation in veterinary medicine. Vet J, 185, 23-27.
- Eckersall PD, Conner JG (1988): Bovine and canine acute phase proteins. Vet Res Commun, 12, 169-178.
- Enemark HL, Bille-Hansen V, Lind P ve ark. (2003): Pathogenicity of Cryptosporidium parvum-evaluation of an animal infection model. Vet Parasitol, 113, 35-57.
- Ganheim C, Höglund J, Waller KP (2004): Acute phase proteins in response to Dictyocaulus viviparus infection in calves. Acta Vet Scand, 45, 79-86.
- Glass EJ, Craigmile SC, Springbett A ve ark. (2003): The protozoan parasite, Theileria annulata, induces a distinct acute phase protein response in cattle that is associated with pathology. Int J Parasitol, 33, 1409-1418.
- Gruys E, Toussaint MJM, Niewold TA ve ark. (2005): Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci B, 6, 1045-1056.
- Gutteridge JM (1993): Free radicals in disease processes: A compilation of cause and consequence. Free Radic Res Commun, 19, 141-158.
- Hayat CS, Khalid M, Iqbal Z ve ark. (1999): Haematological and biochemical disturbance associated with Toxocara vitulorum infection in buffalo calves. Int J Agri Biol, 4, 247-249.
- Kaneko JJ, Harvey JW, Bruss ML (2008): Clinical Biochemistry of Domestic Animals. Sixth Ed., Academic Press, New York, pp 364-390.
- Katz D, Mazor D, Dvilansky A ve ark. (1996): Effects of radiation on red cell membrane and intracelluler oxidative defense systems. Free Radic Res, 24, 199-204.
- Kolodziejczyk L, Siemieniuk E, Skrzydlewska E (2005): Antioxidant potential of rat liver in experimental infection with Fasciola hepatica. Parasitol Res, 96, 367-372.
- Kozan E, Avcı G, Sevimli Kırcalı F ve ark. (2010): Determine of the antioxidant levels and some biochemical parameters on infected with ascaridiosis and treated dogs. Vet J Ankara Univ, 57, 93-97.
- Lobetti RG, Möhr AJ, Dippenaar T ve ark. (2000): A preliminary study on the serum protein response in canine babesiosis. J S Afr Vet Assoc, 71, 38-42.
- Özcel MA (2013): Veteriner Hekimliğinde Parazit Hastalıkları, First Ed., Meta basım, İzmir, pp 233-237.
- Özkurt G, Gökçen A, Çamkerten İ ve ark. (2012): Erythrocyte SOD, CAT, GPx enzymes activity and MDA level in Kilis goats with naturally-occurred nematodiasis. Harran Univ Vet Med J, 1, 107-110.
- Petersen HH, Nielsen JP, Heegaard PM (2004): Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res, 35, 163-187.
- Radostits OM, Blood DC, Gay CC (1994): Askarid infections. In: Radostits, O.M., Blood, D.C., Gay, C.C., (Eds.), Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses, 8th edition, London, Saunders Company, pp 1556-1558.
- Russell KE, Roussel AJ (2007): Evaluation of the ruminant serum chemistry profile. Vet Clin Food Anim, 23, 403-426.
- Sarma K, Saravanan M, Mondal DB ve ark. (2012): Influence of natural infection of Toxocara vitulorum on markers of oxidative stress in Indian buffalo calves. Indian J Anim Sci, 82, 1142-1145.
- Sezer K, Keskin M (2014): Role of the free oxygen radicals on the pathogenesis of the diseases. Fırat Univ Vet J Health Sci, 28, 49-56.
- Skinner JG, Brown RA, Roberts L (1991): Bovine haptoglobin conditions.Vet Rec, 128, 147-149. clinically defined field
- SPSS (2008): SPSS for windows release 16.0.2. SPSS Inc., Chicago
- Tabakoğlu E, Durgut R (2013): Oxidative stress in veterinary medicine and effects in some important diseases. AVKAE J, 3, 69-75.
- Tothova C, Nagy O, Kovac G (2014): Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med, 59, 163-180.
- Toussaint MJM, van Ederen AM, Gruys E (1995): Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Int, 5, 149-157.
- Ulutas B, Bayramli G, Ulutas PA ve ark. (2005): Serum concentration of some acute phase proteins in naturally occuring canine babesiosis: Preliminary studies. Vet Clin Pathol, 34, 144-147.
- Ulutaş PA, Voyvoda H, Ulutaş B ve ark. (2008): Haptoglobin, concentrations in goats with mixed helminth infection. Turkiye Parazitol Derg, 32, 229-233. and ceruloplasmin
- Urquhart GM, Armour J, Duncan JL ve ark. (1996): Veterinary Parasitology. 2nd Ed., Blackwell Scientific Publications, Oxford, pp 270.
- Van Der Steen L, Pardon B, Sarre C ve ark. (2014): Intestinal obstruction by Toxocara vitulorum in a calf. Vlaams Diergen Tijds, 83, 299-305.
- Yoshioka T, Kawada K, Shimada T ve ark. (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376. Geliş tarihi: 03.02.2016 /Kabul tarihi:18.08.2016
There are 40 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA53CE93YS |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2017 |
| Published in Issue | Year 2017Volume: 64 Issue: 2 |