Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings

Year 2017, Volume: 64 Issue: 2, 99 - 102, 01.06.2017

Ayşe DOĞAN Mine ERİŞİR

https://doi.org/10.1501/Vetfak_0000002782

Abstract

This study examined the effect of Aroclor 1254 on arginase activity and protective role of vitamin E among adult rats, pregnant rats and their offsprings. Totally 90 Sprague-Dawley rats were divided into three main groups as 30 pregnant, 30 adult and 30 offsprings (pups of the pregnant rats) based on the treatments administered during the pregnancy period (20 days) with subcutaneous daily injections of Aroclor 1254 (2 mg/kg/day) alone or associated with vitamin E (50 mg/kg/day) or normal saline in controls. Female rats and their offsprings (10 offsprings per groups) were analyzed in terms of the arginase activity in liver. A statistically significant increase was determined in liver arginase activity of adult rats exposed to Aroclor 1254 (p<0.05). Vitamin E administered simultaneously with Aroclor enabled liver arginase activity of adult rats to approach statistically normal values. A statistically insignificant increase was found in the arginase activity among pregnant rats and offsprings. While vitamin E administered simultaneously with Aroclor was ineffective in pregnant rats, it caused a statistically significant increase in offsprings (p<0.05). We recommend that addition of vitamin E can prevent the increase in the liver arginase activity caused by Aroclor 1254

Keywords

Arginase, Aroclor 1254, offspring, pregnant, vitamin E

References

  • Agency for Toxic Substances and Disease Registry (ATSDR): Toxicological Profile for Polychlorinated Biphenyls (PCBs) (2000): US Dept Health Services. Public Health Service, Atlanta.
  • Ando M, Saito H, Wakisaka I (1986): Gas chromatographic and mass spectrometric analysis of polychlorinated biphenyls in human placenta and cord blood. Environ Res, 41, 14-22.
  • Ash DE (2004): Structure and function of arginases. J Nutr, 134, 2760-2767.
  • Carpenter DO (1998): Polychlorinated biphenyls and human health. Int J Occup Med Environ Health, 11, 291-303.
  • Carpenter DO, Stoner CT, Lawrence DA, et al. (1996): Multiple mechanisms of PCB neurotoxicity. Proc Pacific Basin Conference on Hazardous Waste. Kuala Lumpur, Malaysia, 404-418.
  • Chen CJ, Yu ML, Rogan WJ, et al. (1994): A 6-year follow-up of behavior and activity disorders in the Taiwan Yu-cheng children. Am J Public Health, 84, 415-421.
  • Chrzanowska A, Graboń W, Mielczarek-Puta M, et al. (2014): Significance of arginase determination in body fluids of patients with hepatocellular carcinoma and liver cirrhosis before and after surgical treatment. Clin Biochem, 47, 1056-1059.
  • Chu I, Villeneuve DC, Yagminas A, et al. (1994): Subchronic toxicity of 3,3’,4,4’,5-pentachlorobiphenyl in the rat. I. Clinical, biochemical, hematological, and histopathological changes. Fundam Appl Toxicol, 22, 457- 468.
  • Dundar Y, Aslan R (1999): Bir antioksidan olarak vitamin E. Genel Tıp Derg, 9, 109-116.
  • Ebner K, Couri D (1988): Aroclor 1254 treatment and fasting influences on rat liver mitochondrial carbamoyl phosphate synthesis with ADP and ATP. Toxicol Appl Pharmacol, 96, 75-84.
  • Ebner KV, Bergen WG, Braselton WE Jr (1987): Nitrogen deficits in aroclor 1254-treated rats. Fundam Appl Toxicol, 8, 89-96.
  • Erisir M, Beytut E, Ozan S, et al. (2003): Effects of dietary vitamin E and selenium on arginase activity in the liver, kidney and heart of rats treated with high doses of glucocorticoids. Cell Biochem Funct, 21, 331-335.
  • Gallo-Torres HE (1980): Absorbtion, blood transport and metabolism of vitamin E. 170-267. In: Machlin LJ (Ed), Comprehensive Treatise. Marcel-Decker, New York.
  • Geyer JW, Dabich D (1971): Rapid method for determination of arginase activity in tissue homogenates. Anal Biochem, 39, 412-417.
  • Hansen LG (1999): The Ortho Side of PCBs. Occurrence and Disposition. Kluwer Academic Publishers, Boston.
  • Herrera E, Knopp RH, Freinkel N (1969): Carbohydrate metabolism in pregnancy. VI. Plasma fuels, insulin, liver composition, gluconeogenesis and nitrogen metabolism during late gestation in the fed and fasted rats. J Clin Invest, 48, 2260-2272.
  • Kimbrough RD, Krouskas CA (2003): Human exposure to polychlorinated biphenyls and health effects: A critical synopsis. Toxicol Rev, 22, 217-233.
  • Kutlu S, Colakoglu N, Halifeoglu I, et al. (2007): Comparative evaluation of hepatotoxic and nephrotoxic effects of aroclors 1221 and 1254 in female rats. Cell Biochem Funct, 25, 167-172.
  • Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951): Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
  • Lu CF, Wang YM, Peng SQ, et al. (2009): Combined effects of repeated
  • administration of 2,3,7,8- Effects of diethyl maleate,
  • Powers GS, Meister T (1982): Urea synthesis and ammonia metabolism. 251-263. In: Arias I, Popper H, Schachter D, Shafrits DA, editors. The liver: Biology and Pathobiology. Raven Press, New York.
  • Robertson LW, Hansen LG (2001): PCBs: Recent Advances in the Environmental Toxicology and Health Effects. The University Press of Kentucky, Lexington.
  • Safe S (1994): Polychlorinated biphenyls (PCBs): Environmental impact, biochemical and toxic responses, and implications for risk assessment. Crit Rev Toxicol, 24, 87-149.
  • Sonne C, Riget FF, Leat EHK, et al. (2013): Organohalogen contaminants and blood plasma clinical- chemical parameters in three colonies of North Atlantic Great skua (Stercorarius skua). Ecotox Environ Safe, 92, 245-251.
  • Takagi Y, Aburada S, Hashimoto K, et al. (1986): Transfer polychlorinated biphenyls from maternal to fetal and suckling rats. Arch Environ Contam Toxicol, 15, 709-715.
  • Ulbrich B, Stahlmann R (2004): Developmental toxicity of polychlorinated biphenyls (PCBs): A systematic review of experimental data. Arch Toxicol, 78, 252-268.

Aroclor 1254 ve vitamin E’nin yetişkin, gebe rat ve yavrularında arginaz aktivitesi üzerine etkileri

Year 2017, Volume: 64 Issue: 2, 99 - 102, 01.06.2017

Ayşe DOĞAN Mine ERİŞİR

https://doi.org/10.1501/Vetfak_0000002782

Abstract

Bu çalışmada yetişkin, gebe rat ve yavrularında arginaz aktivitesi üzerine Aroclor 1254’ün etkisi ve vitamin E’nin koruyucu rolü araştırıldı. Toplam 90 Sprague-Dawley rat 30 yetişkin, 30 gebe ve 30 yavru olmak üzere üç ana gruba ayrıldı. Gebelik süresince (20 gün) subkutan olarak Aroclor 1254 (2 mg/kg/gün) tek başına veya vitamin E (50 mg/kg/gün) ile birlikte ve kontrol grubuna serum fizyolojik uygulandı. Dişi ratlar ve onların yavrularının (her gruptan 10 yavru) karaciğerinde arginaz aktivitesi analiz edildi. Aroclor 1254’e maruz kalan yetişkin ratların karaciğer arginaz aktivitesinde anlamlı bir artış (p<0.05) belirlendi. Aroclor 1254 ile birlikte vitamin E uygulanan yetişkin ratların arginaz aktivitesi istatistiksel olarak normal değerlere yaklaştı. Gebe ratlar ve yavrularının arginaz aktivitesinde istatistiksel olarak anlamsız bir artış tespit edildi. Aroclor 1254 ile birlikte vitamin E uygulanması gebe ratlarda etkili olmasa da; yavru ratlarda anlamlı bir artışa (p<0.05) sebep olmuştur. Vitamin E ilavesinin, Aroclor 1254’ün sebep olduğu karaciğer arginaz aktivitesindeki artışı önleyebildiğini önermekteyiz

Keywords

Arginaz, Aroclor 1254, gebe, vitamin E, yavru

References

  • Agency for Toxic Substances and Disease Registry (ATSDR): Toxicological Profile for Polychlorinated Biphenyls (PCBs) (2000): US Dept Health Services. Public Health Service, Atlanta.
  • Ando M, Saito H, Wakisaka I (1986): Gas chromatographic and mass spectrometric analysis of polychlorinated biphenyls in human placenta and cord blood. Environ Res, 41, 14-22.
  • Ash DE (2004): Structure and function of arginases. J Nutr, 134, 2760-2767.
  • Carpenter DO (1998): Polychlorinated biphenyls and human health. Int J Occup Med Environ Health, 11, 291-303.
  • Carpenter DO, Stoner CT, Lawrence DA, et al. (1996): Multiple mechanisms of PCB neurotoxicity. Proc Pacific Basin Conference on Hazardous Waste. Kuala Lumpur, Malaysia, 404-418.
  • Chen CJ, Yu ML, Rogan WJ, et al. (1994): A 6-year follow-up of behavior and activity disorders in the Taiwan Yu-cheng children. Am J Public Health, 84, 415-421.
  • Chrzanowska A, Graboń W, Mielczarek-Puta M, et al. (2014): Significance of arginase determination in body fluids of patients with hepatocellular carcinoma and liver cirrhosis before and after surgical treatment. Clin Biochem, 47, 1056-1059.
  • Chu I, Villeneuve DC, Yagminas A, et al. (1994): Subchronic toxicity of 3,3’,4,4’,5-pentachlorobiphenyl in the rat. I. Clinical, biochemical, hematological, and histopathological changes. Fundam Appl Toxicol, 22, 457- 468.
  • Dundar Y, Aslan R (1999): Bir antioksidan olarak vitamin E. Genel Tıp Derg, 9, 109-116.
  • Ebner K, Couri D (1988): Aroclor 1254 treatment and fasting influences on rat liver mitochondrial carbamoyl phosphate synthesis with ADP and ATP. Toxicol Appl Pharmacol, 96, 75-84.
  • Ebner KV, Bergen WG, Braselton WE Jr (1987): Nitrogen deficits in aroclor 1254-treated rats. Fundam Appl Toxicol, 8, 89-96.
  • Erisir M, Beytut E, Ozan S, et al. (2003): Effects of dietary vitamin E and selenium on arginase activity in the liver, kidney and heart of rats treated with high doses of glucocorticoids. Cell Biochem Funct, 21, 331-335.
  • Gallo-Torres HE (1980): Absorbtion, blood transport and metabolism of vitamin E. 170-267. In: Machlin LJ (Ed), Comprehensive Treatise. Marcel-Decker, New York.
  • Geyer JW, Dabich D (1971): Rapid method for determination of arginase activity in tissue homogenates. Anal Biochem, 39, 412-417.
  • Hansen LG (1999): The Ortho Side of PCBs. Occurrence and Disposition. Kluwer Academic Publishers, Boston.
  • Herrera E, Knopp RH, Freinkel N (1969): Carbohydrate metabolism in pregnancy. VI. Plasma fuels, insulin, liver composition, gluconeogenesis and nitrogen metabolism during late gestation in the fed and fasted rats. J Clin Invest, 48, 2260-2272.
  • Kimbrough RD, Krouskas CA (2003): Human exposure to polychlorinated biphenyls and health effects: A critical synopsis. Toxicol Rev, 22, 217-233.
  • Kutlu S, Colakoglu N, Halifeoglu I, et al. (2007): Comparative evaluation of hepatotoxic and nephrotoxic effects of aroclors 1221 and 1254 in female rats. Cell Biochem Funct, 25, 167-172.
  • Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951): Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
  • Lu CF, Wang YM, Peng SQ, et al. (2009): Combined effects of repeated
  • administration of 2,3,7,8- Effects of diethyl maleate,
  • Powers GS, Meister T (1982): Urea synthesis and ammonia metabolism. 251-263. In: Arias I, Popper H, Schachter D, Shafrits DA, editors. The liver: Biology and Pathobiology. Raven Press, New York.
  • Robertson LW, Hansen LG (2001): PCBs: Recent Advances in the Environmental Toxicology and Health Effects. The University Press of Kentucky, Lexington.
  • Safe S (1994): Polychlorinated biphenyls (PCBs): Environmental impact, biochemical and toxic responses, and implications for risk assessment. Crit Rev Toxicol, 24, 87-149.
  • Sonne C, Riget FF, Leat EHK, et al. (2013): Organohalogen contaminants and blood plasma clinical- chemical parameters in three colonies of North Atlantic Great skua (Stercorarius skua). Ecotox Environ Safe, 92, 245-251.
  • Takagi Y, Aburada S, Hashimoto K, et al. (1986): Transfer polychlorinated biphenyls from maternal to fetal and suckling rats. Arch Environ Contam Toxicol, 15, 709-715.
  • Ulbrich B, Stahlmann R (2004): Developmental toxicity of polychlorinated biphenyls (PCBs): A systematic review of experimental data. Arch Toxicol, 78, 252-268.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA48HE24ZP
Journal Section Research Article
Authors

Ayşe DOĞAN
Bitlis Eren University, Academy of Medicine, Department of Physiotherapy and Rehabilitation, Bitlis

Mine ERİŞİR
Bitlis Eren University, Academy of Medicine, Department of Physiotherapy and Rehabilitation, Bitlis

Publication Date June 1, 2017
Published in Issue Year 2017Volume: 64 Issue: 2

Cite

Bibtex @letter to editor { auvfd526113, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2017}, volume = {64}, number = {2}, pages = {99 - 102}, doi = {10.1501/Vetfak\_0000002782}, title = {Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings}, key = {cite}, author = {Doğan, Ayşe and Erişir, Mine} }
APA Doğan, A. & Erişir, M. (2017). Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 64 (2) , 99-102 . DOI: 10.1501/Vetfak_0000002782
MLA Doğan, A. , Erişir, M. "Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 99-102 <http://vetjournal.ankara.edu.tr/en/pub/issue/43289/526113>
Chicago Doğan, A. , Erişir, M. "Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 99-102
RIS TY - JOUR T1 - Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings AU - AyşeDoğan, MineErişir Y1 - 2017 PY - 2017 N1 - doi: 10.1501/Vetfak_0000002782 DO - 10.1501/Vetfak_0000002782 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 99 EP - 102 VL - 64 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002782 UR - https://doi.org/10.1501/Vetfak_0000002782 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings %A Ayşe Doğan , Mine Erişir %T Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings %D 2017 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 64 %N 2 %R doi: 10.1501/Vetfak_0000002782 %U 10.1501/Vetfak_0000002782
ISNAD Doğan, Ayşe , Erişir, Mine . "Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 / 2 (June 2017): 99-102 . https://doi.org/10.1501/Vetfak_0000002782
AMA Doğan A. , Erişir M. Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings. Ankara Univ Vet Fak Derg. 2017; 64(2): 99-102.
Vancouver Doğan A. , Erişir M. Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64(2): 99-102.
IEEE A. Doğan and M. Erişir , "Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 64, no. 2, pp. 99-102, Jun. 2017, doi:10.1501/Vetfak_0000002782