Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings
Abstract
This study examined the effect of Aroclor 1254 on arginase activity and protective role of vitamin E among adult rats, pregnant rats and their offsprings. Totally 90 Sprague-Dawley rats were divided into three main groups as 30 pregnant, 30 adult and 30 offsprings (pups of the pregnant rats) based on the treatments administered during the pregnancy period (20 days) with subcutaneous daily injections of Aroclor 1254 (2 mg/kg/day) alone or associated with vitamin E (50 mg/kg/day) or normal saline in controls. Female rats and their offsprings (10 offsprings per groups) were analyzed in terms of the arginase activity in liver. A statistically significant increase was determined in liver arginase activity of adult rats exposed to Aroclor 1254 (p<0.05). Vitamin E administered simultaneously with Aroclor enabled liver arginase activity of adult rats to approach statistically normal values. A statistically insignificant increase was found in the arginase activity among pregnant rats and offsprings. While vitamin E administered simultaneously with Aroclor was ineffective in pregnant rats, it caused a statistically significant increase in offsprings (p<0.05). We recommend that addition of vitamin E can prevent the increase in the liver arginase activity caused by Aroclor 1254
Keywords
References
- Agency for Toxic Substances and Disease Registry (ATSDR): Toxicological Profile for Polychlorinated Biphenyls (PCBs) (2000): US Dept Health Services. Public Health Service, Atlanta.
- Ando M, Saito H, Wakisaka I (1986): Gas chromatographic and mass spectrometric analysis of polychlorinated biphenyls in human placenta and cord blood. Environ Res, 41, 14-22.
- Ash DE (2004): Structure and function of arginases. J Nutr, 134, 2760-2767.
- Carpenter DO (1998): Polychlorinated biphenyls and human health. Int J Occup Med Environ Health, 11, 291-303.
- Carpenter DO, Stoner CT, Lawrence DA, et al. (1996): Multiple mechanisms of PCB neurotoxicity. Proc Pacific Basin Conference on Hazardous Waste. Kuala Lumpur, Malaysia, 404-418.
- Chen CJ, Yu ML, Rogan WJ, et al. (1994): A 6-year follow-up of behavior and activity disorders in the Taiwan Yu-cheng children. Am J Public Health, 84, 415-421.
- Chrzanowska A, Graboń W, Mielczarek-Puta M, et al. (2014): Significance of arginase determination in body fluids of patients with hepatocellular carcinoma and liver cirrhosis before and after surgical treatment. Clin Biochem, 47, 1056-1059.
- Chu I, Villeneuve DC, Yagminas A, et al. (1994): Subchronic toxicity of 3,3’,4,4’,5-pentachlorobiphenyl in the rat. I. Clinical, biochemical, hematological, and histopathological changes. Fundam Appl Toxicol, 22, 457- 468.
- Dundar Y, Aslan R (1999): Bir antioksidan olarak vitamin E. Genel Tıp Derg, 9, 109-116.
- Ebner K, Couri D (1988): Aroclor 1254 treatment and fasting influences on rat liver mitochondrial carbamoyl phosphate synthesis with ADP and ATP. Toxicol Appl Pharmacol, 96, 75-84.
- Ebner KV, Bergen WG, Braselton WE Jr (1987): Nitrogen deficits in aroclor 1254-treated rats. Fundam Appl Toxicol, 8, 89-96.
- Erisir M, Beytut E, Ozan S, et al. (2003): Effects of dietary vitamin E and selenium on arginase activity in the liver, kidney and heart of rats treated with high doses of glucocorticoids. Cell Biochem Funct, 21, 331-335.
- Gallo-Torres HE (1980): Absorbtion, blood transport and metabolism of vitamin E. 170-267. In: Machlin LJ (Ed), Comprehensive Treatise. Marcel-Decker, New York.
- Geyer JW, Dabich D (1971): Rapid method for determination of arginase activity in tissue homogenates. Anal Biochem, 39, 412-417.
- Hansen LG (1999): The Ortho Side of PCBs. Occurrence and Disposition. Kluwer Academic Publishers, Boston.
- Herrera E, Knopp RH, Freinkel N (1969): Carbohydrate metabolism in pregnancy. VI. Plasma fuels, insulin, liver composition, gluconeogenesis and nitrogen metabolism during late gestation in the fed and fasted rats. J Clin Invest, 48, 2260-2272.
- Kimbrough RD, Krouskas CA (2003): Human exposure to polychlorinated biphenyls and health effects: A critical synopsis. Toxicol Rev, 22, 217-233.
- Kutlu S, Colakoglu N, Halifeoglu I, et al. (2007): Comparative evaluation of hepatotoxic and nephrotoxic effects of aroclors 1221 and 1254 in female rats. Cell Biochem Funct, 25, 167-172.
- Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951): Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
- Lu CF, Wang YM, Peng SQ, et al. (2009): Combined effects of repeated
- administration of 2,3,7,8- Effects of diethyl maleate,
- Powers GS, Meister T (1982): Urea synthesis and ammonia metabolism. 251-263. In: Arias I, Popper H, Schachter D, Shafrits DA, editors. The liver: Biology and Pathobiology. Raven Press, New York.
- Robertson LW, Hansen LG (2001): PCBs: Recent Advances in the Environmental Toxicology and Health Effects. The University Press of Kentucky, Lexington.
- Safe S (1994): Polychlorinated biphenyls (PCBs): Environmental impact, biochemical and toxic responses, and implications for risk assessment. Crit Rev Toxicol, 24, 87-149.
- Sonne C, Riget FF, Leat EHK, et al. (2013): Organohalogen contaminants and blood plasma clinical- chemical parameters in three colonies of North Atlantic Great skua (Stercorarius skua). Ecotox Environ Safe, 92, 245-251.
- Takagi Y, Aburada S, Hashimoto K, et al. (1986): Transfer polychlorinated biphenyls from maternal to fetal and suckling rats. Arch Environ Contam Toxicol, 15, 709-715.
- Ulbrich B, Stahlmann R (2004): Developmental toxicity of polychlorinated biphenyls (PCBs): A systematic review of experimental data. Arch Toxicol, 78, 252-268.
Aroclor 1254 ve vitamin E’nin yetişkin, gebe rat ve yavrularında arginaz aktivitesi üzerine etkileri
Abstract
Bu çalışmada yetişkin, gebe rat ve yavrularında arginaz aktivitesi üzerine Aroclor 1254’ün etkisi ve vitamin E’nin koruyucu rolü araştırıldı. Toplam 90 Sprague-Dawley rat 30 yetişkin, 30 gebe ve 30 yavru olmak üzere üç ana gruba ayrıldı. Gebelik süresince (20 gün) subkutan olarak Aroclor 1254 (2 mg/kg/gün) tek başına veya vitamin E (50 mg/kg/gün) ile birlikte ve kontrol grubuna serum fizyolojik uygulandı. Dişi ratlar ve onların yavrularının (her gruptan 10 yavru) karaciğerinde arginaz aktivitesi analiz edildi. Aroclor 1254’e maruz kalan yetişkin ratların karaciğer arginaz aktivitesinde anlamlı bir artış (p<0.05) belirlendi. Aroclor 1254 ile birlikte vitamin E uygulanan yetişkin ratların arginaz aktivitesi istatistiksel olarak normal değerlere yaklaştı. Gebe ratlar ve yavrularının arginaz aktivitesinde istatistiksel olarak anlamsız bir artış tespit edildi. Aroclor 1254 ile birlikte vitamin E uygulanması gebe ratlarda etkili olmasa da; yavru ratlarda anlamlı bir artışa (p<0.05) sebep olmuştur. Vitamin E ilavesinin, Aroclor 1254’ün sebep olduğu karaciğer arginaz aktivitesindeki artışı önleyebildiğini önermekteyiz
Keywords
References
- Agency for Toxic Substances and Disease Registry (ATSDR): Toxicological Profile for Polychlorinated Biphenyls (PCBs) (2000): US Dept Health Services. Public Health Service, Atlanta.
- Ando M, Saito H, Wakisaka I (1986): Gas chromatographic and mass spectrometric analysis of polychlorinated biphenyls in human placenta and cord blood. Environ Res, 41, 14-22.
- Ash DE (2004): Structure and function of arginases. J Nutr, 134, 2760-2767.
- Carpenter DO (1998): Polychlorinated biphenyls and human health. Int J Occup Med Environ Health, 11, 291-303.
- Carpenter DO, Stoner CT, Lawrence DA, et al. (1996): Multiple mechanisms of PCB neurotoxicity. Proc Pacific Basin Conference on Hazardous Waste. Kuala Lumpur, Malaysia, 404-418.
- Chen CJ, Yu ML, Rogan WJ, et al. (1994): A 6-year follow-up of behavior and activity disorders in the Taiwan Yu-cheng children. Am J Public Health, 84, 415-421.
- Chrzanowska A, Graboń W, Mielczarek-Puta M, et al. (2014): Significance of arginase determination in body fluids of patients with hepatocellular carcinoma and liver cirrhosis before and after surgical treatment. Clin Biochem, 47, 1056-1059.
- Chu I, Villeneuve DC, Yagminas A, et al. (1994): Subchronic toxicity of 3,3’,4,4’,5-pentachlorobiphenyl in the rat. I. Clinical, biochemical, hematological, and histopathological changes. Fundam Appl Toxicol, 22, 457- 468.
- Dundar Y, Aslan R (1999): Bir antioksidan olarak vitamin E. Genel Tıp Derg, 9, 109-116.
- Ebner K, Couri D (1988): Aroclor 1254 treatment and fasting influences on rat liver mitochondrial carbamoyl phosphate synthesis with ADP and ATP. Toxicol Appl Pharmacol, 96, 75-84.
- Ebner KV, Bergen WG, Braselton WE Jr (1987): Nitrogen deficits in aroclor 1254-treated rats. Fundam Appl Toxicol, 8, 89-96.
- Erisir M, Beytut E, Ozan S, et al. (2003): Effects of dietary vitamin E and selenium on arginase activity in the liver, kidney and heart of rats treated with high doses of glucocorticoids. Cell Biochem Funct, 21, 331-335.
- Gallo-Torres HE (1980): Absorbtion, blood transport and metabolism of vitamin E. 170-267. In: Machlin LJ (Ed), Comprehensive Treatise. Marcel-Decker, New York.
- Geyer JW, Dabich D (1971): Rapid method for determination of arginase activity in tissue homogenates. Anal Biochem, 39, 412-417.
- Hansen LG (1999): The Ortho Side of PCBs. Occurrence and Disposition. Kluwer Academic Publishers, Boston.
- Herrera E, Knopp RH, Freinkel N (1969): Carbohydrate metabolism in pregnancy. VI. Plasma fuels, insulin, liver composition, gluconeogenesis and nitrogen metabolism during late gestation in the fed and fasted rats. J Clin Invest, 48, 2260-2272.
- Kimbrough RD, Krouskas CA (2003): Human exposure to polychlorinated biphenyls and health effects: A critical synopsis. Toxicol Rev, 22, 217-233.
- Kutlu S, Colakoglu N, Halifeoglu I, et al. (2007): Comparative evaluation of hepatotoxic and nephrotoxic effects of aroclors 1221 and 1254 in female rats. Cell Biochem Funct, 25, 167-172.
- Lowry OH, Rosenbrough NJ, Farr AL, et al. (1951): Protein measurements with the folin phenol reagent. J Biol Chem, 193, 265-275.
- Lu CF, Wang YM, Peng SQ, et al. (2009): Combined effects of repeated
- administration of 2,3,7,8- Effects of diethyl maleate,
- Powers GS, Meister T (1982): Urea synthesis and ammonia metabolism. 251-263. In: Arias I, Popper H, Schachter D, Shafrits DA, editors. The liver: Biology and Pathobiology. Raven Press, New York.
- Robertson LW, Hansen LG (2001): PCBs: Recent Advances in the Environmental Toxicology and Health Effects. The University Press of Kentucky, Lexington.
- Safe S (1994): Polychlorinated biphenyls (PCBs): Environmental impact, biochemical and toxic responses, and implications for risk assessment. Crit Rev Toxicol, 24, 87-149.
- Sonne C, Riget FF, Leat EHK, et al. (2013): Organohalogen contaminants and blood plasma clinical- chemical parameters in three colonies of North Atlantic Great skua (Stercorarius skua). Ecotox Environ Safe, 92, 245-251.
- Takagi Y, Aburada S, Hashimoto K, et al. (1986): Transfer polychlorinated biphenyls from maternal to fetal and suckling rats. Arch Environ Contam Toxicol, 15, 709-715.
- Ulbrich B, Stahlmann R (2004): Developmental toxicity of polychlorinated biphenyls (PCBs): A systematic review of experimental data. Arch Toxicol, 78, 252-268.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA48HE24ZP |
| Journal Section | Research Article |
| Authors | |
| Publication Date | June 1, 2017 |
| Published in Issue | Year 2017Volume: 64 Issue: 2 |