Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

Year 2017, Volume: 64 Issue: 2, 125 - 130, 01.06.2017

Buket BAKIR Dinçer ERDAĞ Yildiz Sevda ELİŞ Sari Ebru KARADAĞ Hasan ASKER Mahmut SÖZMEN

https://doi.org/10.1501/Vetfak_0000002785
Cited By: 3

Abstract

The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. A total of 48 male mice (Mus musculus) were used in our study. Mice were divided 6 groups (control group, maize oil group, normal saline group, Onosma nigricaule group, malathion group, Onosma nigrcaule+malathion group). Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. The localization of CAT and Mn-SOD in the renal tissue was determined using the method of streptavidinbiotin-peroxidase. CAT immunoreactivity was determined with a weak intensity in epithelium of renal tubulus proximalis of mice in the malathion group, with a moderate intensity in Onosma nigricaule+malathion group and with a higher intensity in tubulus proximalis of other groups. A cytoplasmic Mn-SOD immunoreactivity was determined with weak intensity in renal medulla of mice in malathion group, with moderate intensity in renal medulla of mice in Onosma nigricaule plant extract+malathion group, maize oil group, and normal saline group and with highly intensity in control and Onosma nigricaule groups. It was concluded that Onosma nigricaule might play a protective role as an antioxidant against the oxidant features of malathion

Keywords

Catalase, kidney, malathion, mice, Onosma nigricaule, superoxide dismutase

References

  • Ami BH, Haim SA (1992): Direct effect of phosphamidon on isolated working rat heart electrical and mechanical function. Toxicol Appl Pharm, 110, 429-439.
  • Bingöl SA, Kocamış H (2010): The gene expression by RT- PCR and immunohistochemical expression pattern of catalase in the kidney tissue of both health and diabetic mice. J Fac Vet Med, 16, 825-834.
  • Buratti FM, D’Aniello A, Volpe MT, et al. (2005): Malathion bioactivation in the human liver: The contribution of different cytochrome p450 isoforms. Drug Metab Dispos 33, 295-302.
  • Cadırcı E, Suleyman H, Aksoy H, et al. (2007): Effects of Onosma armeniacum root extract on ethanol-induced oxidative stres in stomach tissue of mice. Chem Biol Interact, 170, 40-48.
  • Erdağ D (2012): The effects of allium czelghauricum (liliaceae), lathyrus karsianus (fabaceae) and onosma nigricaule (boraginaceae) extracts on oxidation parameters in malathion treated mice. Health Science Institute, Kafkas University, PhD thesis.
  • Fadillioglu E, Erdogan H, Sogut S, et al. (2003): Protective effects of erdosteine against doxorubicin- induced cardiomyopathy in mice. J Appl Toxicol, 23, 71-74.
  • Freeman BA, Crapo JD (1982): Biology of disease: Free radicals and tissue injury. Lab Inves, 47, 412-426.
  • Fridovich I (1983): Superoxide radical: An endogenous toxicant. Ann Rev Pharm Toxicol, 23, 239-257.
  • Gerdy GF, Rose HA, Starey NH (1987): Effect of lenght of exposure to malathion on xenobiotic biotransformation in male rat liver. Toxicol Lett, 38, 193-19.
  • Glusczak l, Miron DDS, Moraes BS, et al. (2007): Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver Catfish (Rhamdia Quelen). Comp Biochem Physiol Part C, 146, 519-524.
  • Guest JA, Copley MP, Hormenic KL (1991): Carsinogenic effects of pesticides. Pathol Pharmacol, 73, 387-390.
  • Halliwell B, Gutteridge JMC (1989): Free radicals in biology and medicine. Clarendon Press. Oxford. UK. Last Edition 2007, p: 95.
  • Johkura K, Usuda N, Liang Y, et al. (1998): Immunohistochemical localization of peroxisomal enzymes in developing rat kidney tissues. J Histochem Cytochem, 46, 1161-1173.
  • Keramati V, Jamili S, Ramin M (2010): Effect of diazinon on catalase antioxidant enzyme activity in liver tissue of Rutilus rutilus. Jour Fisher Aqua Sci, 5, 368-367.
  • Kettarman AJ, Pond SM, Becker CE (1987): The effect of carboxylesterase and glutathion S-transferase activities on malathion toxicity in mice. Toxicol Appl Pharmacol, 87, 389-392. of cytochrome P-450
  • Lemos P, Medeiros RS, Zanuncio JC, et al. (2005): Effect of sublethal concentrations of permetherin on ovary activation in the predator. Braz J Biol, 65, 287-290.
  • Luna LG (1968): Manual of Histologic Staining Methods of Armed Forces Institute of Pathology. Third ed. Mc Graw- Hill Book Comp, London.
  • Mates JM, Sanchez-Jimenez F (1999): Antioxidant enzymes and their implications in pathophysiologic processes. Front Biosci, 15, 339-345.
  • McEven FL, Stephenson GL (1989): The use and significiance of pesticides in the environment. John Wiley & Sons Pub, New York, p:538.
  • Morikawa S, Harada T (1969): Immunohistochemical localization of catalase in mammalian tissues. J Histochem Cytochem, 17, 30-35.
  • Mukhopadhyay S, Das SK, Mukherjee S (2004): Expression nontumorigenic and tumorigenic human mammary epithelial cells. J Biomed Biotech, 4, 195-202. gene in
  • Nirmala P, Akira M, Sloane S, et al. (2011): Generation and characterization of a novel kidney-specific manganese superoxide dismutase knockout Mice. Free Radical Biol Med, 51, 406-416.
  • Pant N, Srivastava SP (2003): Testicular and spermatotoxic effects of Quinalphos in mice. J Appl Toxicol, 23, 271-274.
  • Peterson SV, Enghild JJ (2005): Extracellular superoxide dismutase: Structural and functional considerations of a protein shaped by two different disulfide bridge patterns. Biomed Pharmacother, 59, 175-182.
  • Sibilia V, Rindi G, Pagani F, et al. (2003): Ghrelin protects against ethanol-induced gastric ulcers in mice: studies on the mechanisms of action. Endocrinol, 144, 353- 359.
  • Tosun A, Akkol EK, Bahadir O, et al. (2008): Evaluation of antiinflammatory and antinociceptive activities of some Onosma L. species growing in Turkey. J Ethnopharmacol, 120, 378-831.
  • Tru LD (1990): Principles of Immunohistochemistry. True L.D. Atlas of Diagnostic Immunohistopathology. Newyork Press, USA, p: 1-31.
  • Valko M, Rhodes CJ, Moncol J, et al. (2006): Free radicals, metals and antioxidants in oxidative stres-induced cancer. Chem Bioll Interac, 160, 1-40.
  • Weizman Z, Sofer S (1992): Acute pancreatitis in children with anticholinesterase insecticide intoxication. Pediatr, 90, 204-206.

Malathion ve Onosma nigricaule (Boraginaceae)’nin fare böbrek dokusunda katalaz (CAT) vesüperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkileri

Year 2017, Volume: 64 Issue: 2, 125 - 130, 01.06.2017

Buket BAKIR Dinçer ERDAĞ Yildiz Sevda ELİŞ Sari Ebru KARADAĞ Hasan ASKER Mahmut SÖZMEN

https://doi.org/10.1501/Vetfak_0000002785
Cited By: 3

Abstract

Bu çalışmada, tarımda insektisit olarak kullanılan ve farelerde oksidasyon parametrelerine neden olan malathiona karşı Onosma nigricaule’den elde edilen bitki ekstraktlarının immunohistokimyasal yöntemle böbrek dokusunda katalaz (CAT) ve süperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkilerinin saptanması amaçlanmıştır. Çalışmamızda 48 adet erkek (Mus musculus) fare kullanıldı. Fareler 6 gruba (kontrol grubu, mısır yağı grubu, serum fizyolojik grup, Onosma nigricaule grubu, malathion grubu, Onosma nigricaule+malathion grubu) ayrıldı. Histolojik ve patolojik değerlendirmeler için hematoksilen-eozin and triple boyama yöntemleri kullanıldı. Böbrek dokusunda CAT ve Mn-SOD lokalizasyonu için ise streptavidin-biotin- peroxidase yöntemi kullanıldı. CAT immunoreaktivitesinin fare böbrek dokusunun tubulus proksimalisinde olduğu görüldü. CAT immunoreaktivitesinin malathion grubunda zayıf, malathion+Onosma nigricaule grubunda orta yoğunlukta, diğer gruplarda ise yüksek yoğunlukta olduğu tespit edildi. Mn-SOD immunoreaktivitesinin böbrek medullasında olduğu görüldü. Malathion grubunda zayıf, mısır yağı grubu, serum fizyolojik ve Onosma nigricaule+malathion gruplarında orta yoğunlukta, kontrol ve Onosma nigricaule gruplarında ise yüksek yoğunlukta olduğu tespit edildi. Onosma nigricaule’nin malathionun oksidadif etkilerine karşı antioksidan gibi koruyucu bir rol oynadığı sonucuna varılabilir

Keywords

Böbrek, fare, katalaz, malathion, Onosma nigricaule, süperoksit dismutaz

References

  • Ami BH, Haim SA (1992): Direct effect of phosphamidon on isolated working rat heart electrical and mechanical function. Toxicol Appl Pharm, 110, 429-439.
  • Bingöl SA, Kocamış H (2010): The gene expression by RT- PCR and immunohistochemical expression pattern of catalase in the kidney tissue of both health and diabetic mice. J Fac Vet Med, 16, 825-834.
  • Buratti FM, D’Aniello A, Volpe MT, et al. (2005): Malathion bioactivation in the human liver: The contribution of different cytochrome p450 isoforms. Drug Metab Dispos 33, 295-302.
  • Cadırcı E, Suleyman H, Aksoy H, et al. (2007): Effects of Onosma armeniacum root extract on ethanol-induced oxidative stres in stomach tissue of mice. Chem Biol Interact, 170, 40-48.
  • Erdağ D (2012): The effects of allium czelghauricum (liliaceae), lathyrus karsianus (fabaceae) and onosma nigricaule (boraginaceae) extracts on oxidation parameters in malathion treated mice. Health Science Institute, Kafkas University, PhD thesis.
  • Fadillioglu E, Erdogan H, Sogut S, et al. (2003): Protective effects of erdosteine against doxorubicin- induced cardiomyopathy in mice. J Appl Toxicol, 23, 71-74.
  • Freeman BA, Crapo JD (1982): Biology of disease: Free radicals and tissue injury. Lab Inves, 47, 412-426.
  • Fridovich I (1983): Superoxide radical: An endogenous toxicant. Ann Rev Pharm Toxicol, 23, 239-257.
  • Gerdy GF, Rose HA, Starey NH (1987): Effect of lenght of exposure to malathion on xenobiotic biotransformation in male rat liver. Toxicol Lett, 38, 193-19.
  • Glusczak l, Miron DDS, Moraes BS, et al. (2007): Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver Catfish (Rhamdia Quelen). Comp Biochem Physiol Part C, 146, 519-524.
  • Guest JA, Copley MP, Hormenic KL (1991): Carsinogenic effects of pesticides. Pathol Pharmacol, 73, 387-390.
  • Halliwell B, Gutteridge JMC (1989): Free radicals in biology and medicine. Clarendon Press. Oxford. UK. Last Edition 2007, p: 95.
  • Johkura K, Usuda N, Liang Y, et al. (1998): Immunohistochemical localization of peroxisomal enzymes in developing rat kidney tissues. J Histochem Cytochem, 46, 1161-1173.
  • Keramati V, Jamili S, Ramin M (2010): Effect of diazinon on catalase antioxidant enzyme activity in liver tissue of Rutilus rutilus. Jour Fisher Aqua Sci, 5, 368-367.
  • Kettarman AJ, Pond SM, Becker CE (1987): The effect of carboxylesterase and glutathion S-transferase activities on malathion toxicity in mice. Toxicol Appl Pharmacol, 87, 389-392. of cytochrome P-450
  • Lemos P, Medeiros RS, Zanuncio JC, et al. (2005): Effect of sublethal concentrations of permetherin on ovary activation in the predator. Braz J Biol, 65, 287-290.
  • Luna LG (1968): Manual of Histologic Staining Methods of Armed Forces Institute of Pathology. Third ed. Mc Graw- Hill Book Comp, London.
  • Mates JM, Sanchez-Jimenez F (1999): Antioxidant enzymes and their implications in pathophysiologic processes. Front Biosci, 15, 339-345.
  • McEven FL, Stephenson GL (1989): The use and significiance of pesticides in the environment. John Wiley & Sons Pub, New York, p:538.
  • Morikawa S, Harada T (1969): Immunohistochemical localization of catalase in mammalian tissues. J Histochem Cytochem, 17, 30-35.
  • Mukhopadhyay S, Das SK, Mukherjee S (2004): Expression nontumorigenic and tumorigenic human mammary epithelial cells. J Biomed Biotech, 4, 195-202. gene in
  • Nirmala P, Akira M, Sloane S, et al. (2011): Generation and characterization of a novel kidney-specific manganese superoxide dismutase knockout Mice. Free Radical Biol Med, 51, 406-416.
  • Pant N, Srivastava SP (2003): Testicular and spermatotoxic effects of Quinalphos in mice. J Appl Toxicol, 23, 271-274.
  • Peterson SV, Enghild JJ (2005): Extracellular superoxide dismutase: Structural and functional considerations of a protein shaped by two different disulfide bridge patterns. Biomed Pharmacother, 59, 175-182.
  • Sibilia V, Rindi G, Pagani F, et al. (2003): Ghrelin protects against ethanol-induced gastric ulcers in mice: studies on the mechanisms of action. Endocrinol, 144, 353- 359.
  • Tosun A, Akkol EK, Bahadir O, et al. (2008): Evaluation of antiinflammatory and antinociceptive activities of some Onosma L. species growing in Turkey. J Ethnopharmacol, 120, 378-831.
  • Tru LD (1990): Principles of Immunohistochemistry. True L.D. Atlas of Diagnostic Immunohistopathology. Newyork Press, USA, p: 1-31.
  • Valko M, Rhodes CJ, Moncol J, et al. (2006): Free radicals, metals and antioxidants in oxidative stres-induced cancer. Chem Bioll Interac, 160, 1-40.
  • Weizman Z, Sofer S (1992): Acute pancreatitis in children with anticholinesterase insecticide intoxication. Pediatr, 90, 204-206.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA43UH58ZB
Journal Section Research Article
Authors

Buket BAKIR
Namık Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology,

Dinçer ERDAĞ
Namık Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology,

Yildiz Sevda ELİŞ
Namık Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology,

Sari Ebru KARADAĞ
Namık Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology,

Hasan ASKER
Namık Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology,

Mahmut SÖZMEN
Namık Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology,

Publication Date June 1, 2017
Published in Issue Year 2017Volume: 64 Issue: 2

Cite

Bibtex @letter to editor { auvfd526132, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2017}, volume = {64}, number = {2}, pages = {125 - 130}, doi = {10.1501/Vetfak\_0000002785}, title = {Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice}, key = {cite}, author = {Bakır, Buket and Erdağ, Dinçer and Eliş, Yildiz Sevda and Karadağ, Sari Ebru and Asker, Hasan and Sözmen, Mahmut} }
APA Bakır, B. , Erdağ, D. , Eliş, Y. S. , Karadağ, S. E. , Asker, H. & Sözmen, M. (2017). Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 64 (2) , 125-130 . DOI: 10.1501/Vetfak_0000002785
MLA Bakır, B. , Erdağ, D. , Eliş, Y. S. , Karadağ, S. E. , Asker, H. , Sözmen, M. "Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 125-130 <http://vetjournal.ankara.edu.tr/en/pub/issue/43289/526132>
Chicago Bakır, B. , Erdağ, D. , Eliş, Y. S. , Karadağ, S. E. , Asker, H. , Sözmen, M. "Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 (2017 ): 125-130
RIS TY - JOUR T1 - Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice AU - BuketBakır, DinçerErdağ, Yildiz SevdaEliş, Sari EbruKaradağ, HasanAsker, MahmutSözmen Y1 - 2017 PY - 2017 N1 - doi: 10.1501/Vetfak_0000002785 DO - 10.1501/Vetfak_0000002785 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 125 EP - 130 VL - 64 IS - 2 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002785 UR - https://doi.org/10.1501/Vetfak_0000002785 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice %A Buket Bakır , Dinçer Erdağ , Yildiz Sevda Eliş , Sari Ebru Karadağ , Hasan Asker , Mahmut Sözmen %T Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice %D 2017 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 64 %N 2 %R doi: 10.1501/Vetfak_0000002785 %U 10.1501/Vetfak_0000002785
ISNAD Bakır, Buket , Erdağ, Dinçer , Eliş, Yildiz Sevda , Karadağ, Sari Ebru , Asker, Hasan , Sözmen, Mahmut . "Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice". Ankara Üniversitesi Veteriner Fakültesi Dergisi 64 / 2 (June 2017): 125-130 . https://doi.org/10.1501/Vetfak_0000002785
AMA Bakır B. , Erdağ D. , Eliş Y. S. , Karadağ S. E. , Asker H. , Sözmen M. Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice. Ankara Univ Vet Fak Derg. 2017; 64(2): 125-130.
Vancouver Bakır B. , Erdağ D. , Eliş Y. S. , Karadağ S. E. , Asker H. , Sözmen M. Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2017; 64(2): 125-130.
IEEE B. Bakır , D. Erdağ , Y. S. Eliş , S. E. Karadağ , H. Asker and M. Sözmen , "Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 64, no. 2, pp. 125-130, Jun. 2017, doi:10.1501/Vetfak_0000002785

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