Letter to Editor
Year 2015,
Volume: 62 Issue: 4, 255 - 260, 01.12.2015
Abstract
The present study aimed to investigate the caffeic acid phenetyl ester (CAPE) effect on levels of paraoxonase (PON1) activity, oxidative stress index (OSI), total antioxidant (TAS) and total oxidant (TOS) levels in acute poisoning of chlorpyrifos-ethyl (CPF). All experiments conducted on 32 male Mus musculussp. mice divided into 4 equal groups each 25-30 g as following. Group I were treated with saline (% 0.9 NaCl) by intraperitoneal injection (i.p.). Group II with 101 mg/kg CPF plus saline subcutaneously; group III with 10 μmol/kg CAPE intraperitonally; group IV with 101 mg/kg CPF plus 10 μmol/kg CAPE were applied. After serum samples obtained, PON1 activity, OSI, TAS and TOS levels were measured. In this study, PON1 189.21±22.31 U/L; TAS 1.03±0.13 mmol Trolox eqivalen/L; TOS 7.10±0.69 μmol H2O2 equiv./L and OSİ 0.69±0.09 arbitrary unit levels were found during CFP applications. As conclusion, OSI and TOS levels were increased while serum PON1 activity and TAS levels decreased under CPF intoxication compared to group I. Additionally, CAPE was demonstrated the protective effect by reducing CPF-induced oxidative stress
Keywords
Caffeic acid phenetyl ester chlorpyrifos-ethyl oxidative stress paraoxonase total antioxidant
References
- Akgür SA, Oztürk P, Solak I, Moral AR, Ege B (2003): Human serum paraoxonase (PON1) activity in acute organophosphorous insecticide poisoning. Forensic Sci Int, 133, 136-140.
- Alagöz G, Durukan P, Yıldız M, Bayar M.K, Ilhan N, Çevik Y, Seçkin D (2007): Akut zehirlenme hastalarında serum malondialdehid, paraoksonaz ve karaciğer fonksi- yon testleri arasındaki ilişkinin araştırılması. Fırat Tıp Derg., 12, 184-189.
- Alp H, Sak ME, Evsen MS, Fırat U, Evliyaoglu O, Penbegül N, Sancaktutar AA, Söylemez H, Tuzcu M (2012): Effects of malathion in fetal kidney tissues in pregnant rats: teratogenic effects ınduced by different doses. Kafkas Univ Vet Fak Derg., 18, 221-226.
- Ambali FS, Ayo JO, Esievo KAN Ojo SA (2011): Hemo- toxicity Induced by Chronic Chlorpyrifos Exposure in Wistar Rats: Mitigating Effect of Vitamin C. Vet Med Int , Article ID 945439, DOI: 10.4061/2011/945439.
- Bagchi D, Bagchi M, Hassoun EA, Stohs SJ (1995): In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology, 104, 129-140.
- Banskota AH, Tezuka Y, Kadota S (2001): Recent progress in pharmacological research of propolis. Phytother. Res., 15, 561-571.
- Borrelli F, Maffıa P, Pinto L, Ianaro A, Russo A, Capasso F, Ialenti A (2002): Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia, 73, 53-63.
- Castaldo S, Capasso F (2002): Propolis, an old remedy used in modern medicine. Fitoterapia, 73, 1-6.
- Chaudhuri K, Selvaraj S, Pal AK (1999): Studies on the genotoxicity of endosulfan in bacterial systems. Mutation Research, 439, 63-67.
- Cheeseman KH, Slater TF (1993): An introduction to free radical biochemistry. Br. Med. Bull., 49, 479-480.
- Chung MK, Kim JC, Han SS (2002): Developmental toxicity of flupyrazofos, a new organophosphate insectici- de in rats. Food and Chem. Toxicol., 40, 723-729.
- Costa LG, Mcdonald BE, Murphy SD, Omenn GS, Richter RJ, Motulsky AG, Furlong CE (1990): Serum Paraoxonase and its influence on paraoxon and chlorpyri- fos-oxon toxicity in rats. Toxicol. Appl. Pharmacol., 103, 66-76.
- Dwivedi PD, Mukul D, Khanna SK (1998): Role of cytochrome p-450 in quinalphos toxicity : Effect on hepatic and brain antioxidant enzymes in rats. Food and Chem Toxicol., 36, 437-444.
- Eaton DL, Daroff RB, Autrup H, Bridges J, Buffler P, Costa LG, Coyle J, McKhann G, Mobley WC, Nadel L, Neubert D, Schulte-Hermann R, Spencer PS (2008): Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit. Rev. in Toxicol., 38, 1-125.
- Eckerson HW, Romson J, Wyte CM, La Du BN (1983): The Identification of phenotypes by their response to salts. Am. J. Hum. Genet., 35, 214-227. polymorphism:
- Ekebaş S, Çakır S, Ertuğrul O, Kence A (2000): The detection of mutagenic activity of some chemicals (azamethypos, dichlorvos, methyl parathion, aflatoxin b1) by the smart test in drosophila melanogaster. Turk. J. Vet. Anim. Sci., 24, 563-569.
- El-Banna SG, Attia AM, Hafez AM, El-Kazaz SM (2009): Effect of garlic consumption on blood lipid and oxidant/antioxidant parameters in rat males exposed to chlorpyrifos. Slovak J. Anim. Sci., 42, 111-117.
- Erel O (2004): A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable abts radical cation. Clin. Biochem., 37, 277-285.
- Erel O (2005): A new automated colorimetric method for measuring total oxidant status. Clin. Biochem., 38, 1103- 1111.
- Eyer P, Szinicz L, Thiermann H, Worek F, Zilker T (2007): Testing of antidotes for organophosphorus compounds: Experimental procedures and clinical reality. Toxicology, 233, 108-119.
- Gökalp O, Mollaoglu H, Yılmaz HR, Altuntas D (2003): Organofosfat insektisit fenthion’un rat amilaz ve lipaz enzimleri üzerine etkisi: Vitamin E ve C’nin rolü. Süley- man Demirel Üniv. Tıp Fak. Derg., 10, 21-23.
- Gülcü F, Gürsu MF (2003): Paraoksonaz ve aril esteraz aktivite ölçümlerinin standardizasyonu. Turk J. Biochem., 28, 45-49.
- Gültekin F, Oztürk M, Akdogan M (2000): The effect of organophosphate lipid peroxidation and antioxidant enzymes (in vitro). Arch. Toxicol., 74, 533–538.
- chlorpyrifos-ehtyl on
- Gültekin F, Delibaş N, Yaşar S, Kılınç I (2001): In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch. Toxicol., 75, 88-96.
- Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RBG, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS (2004): Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nat. Struct. Mol. Biol., 11, 412-419.
- Ilhan A, Iraz M, Gürel A, Armutcu F, Akyol O (2004): Caffeic acid phenethyl ester exerts a neuroprotective effect on CNS against pentylenetetrazol-induced seizures in mice. Neurochem Res., 29, 2287-2292.
- Jacobsen H, Qstergaard G, Lam HR, Poulsen ME, Frandsen H, Ladefoged O, Meyer O (2004): Repeated dose 28-day oral toxicity study in Wistar rats with a mixture of five pesticides often found as residues in food: alphacypermethrin, chlorpyrifos and mancozeb. Food and Chem. Toxicol., 42, 1269-1277. carbendazim,
- Khan S, Khan, NN (1986): The mobility of some organophosphorus pesticides in soils as affected by some soil parameters. Soil Sci., 142, 214-222.
- Kösecik M, Erel O, Sevinc E, Selek S (2005): Increased oxidative stress in children exposed to passive smoking. Int J Cardiol., 100, 61-64.
- Li WF, Furlong C, Costa LG (1995): Paraoxonase protects against chlorpyrifos toxicity in mice. Toxicol. Lett., 76, 219-226.
- Lodovici M, Cassalini C, Briani C, Dolara P (1997): Oxidative liver DNA damage in rats treated with pesticide mixtures. Toxicology, 117, 55-60.
- Mitra NK, Nadarajah VD, Siong, HH (2009): Effect of concurrent application of heat, swim stres and repeated dermal application of chlorpyrifos on the hippocampal neurons in mice. Folia neuropath., 47, 60-68.
- Ncibi S, Othman MB, Akacha A, Krifi MN, Zourgui L (2008): Opuntia ficus indica extract protects against chlorpyrifos-induced damage on mice liver. Food and Chem Toxicol., 46, 797-802.
- Oncü M, Gültekin F, Karaöz E, Altuntaş I, Delibaş N (2002): Klorprifos-etil tarafından oluşturulan oksidatif ha- sarın sıçan karaciğerine etkileri. T Klin Tıp Bil. Derg., 22, 50-55.
- Peter JV, Moran JL, Graham P (2006): Oxime therapy and outcomes in human organophosphate poisoning: An evaluation using meta-analytic techniques. Crit. Care Med., 34, 502-510.
- Reuber MD (1978): Carcinomas and other lesions of the liver in mice ingestingorganochlorine pesticides. Clinical Toxicol., 13, 231- 256.
- Russo A, Longo R, Vanella A (2002): Antioxidant activity of propolis: Role of caffeic acid phenethyl ester and galangin. Fitoterapia, 73, 21-29.
- Stephen B, Kyle L, Yong X, Cynthia A, Donald E, Earl F, James E (1997): Role of oxidative stress in the mecha- nism of dieldrin's hepatotoxicity. Annals of Clin. and Lab. Sci., 27, 196-208.
- Timchalk C, Busby A, Campbell JA, Needham LL, Barr DN (2007): Comparative pharmacokinetics of the organophosphorus insecticide chlorpyrifos and its major metabolites diethylphosphate, diethylthiophosphate and 3,5,6-trichloro-2-pyridinol in the rat. Toxicology, 237, 145- 157.
- Uzun FG, Demir F, Kalender S, Baş H, Kalender Y (2010): Protective effect of catechin and quercetin on chlorpyrifos-induced lung toxicity in male rats. Food and Chem Toxicol., 48, 1714-1720.
- Watson AD, Berliner JA, Rama SY, La Du BN, Faul KF, Fogelman AM, Navab M (1995): Protective effect of high density lipoprotein associated Paraoxonase: Inhibition of the biological activity of minimally oxidized low density lipoprotein. J. Clin. Invest., 96, 2882-2891. 42. WHO:
- Organophosphorus Pesticides. Erişim
- (http://www.WHO.Org). Erişim Tarihi. 21.03.2013.
Year 2015,
Volume: 62 Issue: 4, 255 - 260, 01.12.2015
Abstract
Bu çalışmada organofosfatlı pestisitlerden biri olan klorprifos-etil (CPF) ile akut zehirlenmede kafeik asit fenetil ester (CAPE)’in paraoksonaz (PON1) aktivitesi, oksidatif stres indeksi (OSİ), total antioksidan (TAS) ve total oksidan (TOS) seviyeleri üzerine etkisinin araştırılması amaçlandı. Çalışmada her biri 25-30 g ağırlığında, 32 adet erkek Mus musculus türü fare rastgele 4 eşit gruba ayrıldı. Grup I’e periton içi enjeksiyon ile serum fizyolojik (% 0.9 NaCl); grup II’ye deri altı serum fizyolojik ile 101 mg/kg CPF; grup III’e periton içi 10 μmol/kg CAPE; grup IV’e ise deri altı 101 mg/kg CPF ve periton içi 10 μmol/kg CAPE uygulandı. Elde edilen serum örneklerinde PON1 aktivitesi, OSİ, TAS ve TOS düzeyleri analiz edildi. Çalışmada CPF uygulamalarında, PON1 189.21±22.31 U/L; TAS 1.03±0.13 mmol Trolox eqivalen/L; TOS 7.10±0.69 μmol H2O2 equiv./L ve OSİ değerleri 0.69±0.09 arbitrary birim olarak bulundu. CPF ile oluşturulan intoksikasyonda PON1 aktivitesi ve TAS düzeyleri azalırken, OSİ ve TOS düzeylerinin arttığı tespit edildi. CAPE’nin ise, CPF’nin neden olduğu oksidatif stresi azaltarak koruyucu etki gösterdiği belirlendi
References
- Akgür SA, Oztürk P, Solak I, Moral AR, Ege B (2003): Human serum paraoxonase (PON1) activity in acute organophosphorous insecticide poisoning. Forensic Sci Int, 133, 136-140.
- Alagöz G, Durukan P, Yıldız M, Bayar M.K, Ilhan N, Çevik Y, Seçkin D (2007): Akut zehirlenme hastalarında serum malondialdehid, paraoksonaz ve karaciğer fonksi- yon testleri arasındaki ilişkinin araştırılması. Fırat Tıp Derg., 12, 184-189.
- Alp H, Sak ME, Evsen MS, Fırat U, Evliyaoglu O, Penbegül N, Sancaktutar AA, Söylemez H, Tuzcu M (2012): Effects of malathion in fetal kidney tissues in pregnant rats: teratogenic effects ınduced by different doses. Kafkas Univ Vet Fak Derg., 18, 221-226.
- Ambali FS, Ayo JO, Esievo KAN Ojo SA (2011): Hemo- toxicity Induced by Chronic Chlorpyrifos Exposure in Wistar Rats: Mitigating Effect of Vitamin C. Vet Med Int , Article ID 945439, DOI: 10.4061/2011/945439.
- Bagchi D, Bagchi M, Hassoun EA, Stohs SJ (1995): In vitro and in vivo generation of reactive oxygen species, DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology, 104, 129-140.
- Banskota AH, Tezuka Y, Kadota S (2001): Recent progress in pharmacological research of propolis. Phytother. Res., 15, 561-571.
- Borrelli F, Maffıa P, Pinto L, Ianaro A, Russo A, Capasso F, Ialenti A (2002): Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia, 73, 53-63.
- Castaldo S, Capasso F (2002): Propolis, an old remedy used in modern medicine. Fitoterapia, 73, 1-6.
- Chaudhuri K, Selvaraj S, Pal AK (1999): Studies on the genotoxicity of endosulfan in bacterial systems. Mutation Research, 439, 63-67.
- Cheeseman KH, Slater TF (1993): An introduction to free radical biochemistry. Br. Med. Bull., 49, 479-480.
- Chung MK, Kim JC, Han SS (2002): Developmental toxicity of flupyrazofos, a new organophosphate insectici- de in rats. Food and Chem. Toxicol., 40, 723-729.
- Costa LG, Mcdonald BE, Murphy SD, Omenn GS, Richter RJ, Motulsky AG, Furlong CE (1990): Serum Paraoxonase and its influence on paraoxon and chlorpyri- fos-oxon toxicity in rats. Toxicol. Appl. Pharmacol., 103, 66-76.
- Dwivedi PD, Mukul D, Khanna SK (1998): Role of cytochrome p-450 in quinalphos toxicity : Effect on hepatic and brain antioxidant enzymes in rats. Food and Chem Toxicol., 36, 437-444.
- Eaton DL, Daroff RB, Autrup H, Bridges J, Buffler P, Costa LG, Coyle J, McKhann G, Mobley WC, Nadel L, Neubert D, Schulte-Hermann R, Spencer PS (2008): Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit. Rev. in Toxicol., 38, 1-125.
- Eckerson HW, Romson J, Wyte CM, La Du BN (1983): The Identification of phenotypes by their response to salts. Am. J. Hum. Genet., 35, 214-227. polymorphism:
- Ekebaş S, Çakır S, Ertuğrul O, Kence A (2000): The detection of mutagenic activity of some chemicals (azamethypos, dichlorvos, methyl parathion, aflatoxin b1) by the smart test in drosophila melanogaster. Turk. J. Vet. Anim. Sci., 24, 563-569.
- El-Banna SG, Attia AM, Hafez AM, El-Kazaz SM (2009): Effect of garlic consumption on blood lipid and oxidant/antioxidant parameters in rat males exposed to chlorpyrifos. Slovak J. Anim. Sci., 42, 111-117.
- Erel O (2004): A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable abts radical cation. Clin. Biochem., 37, 277-285.
- Erel O (2005): A new automated colorimetric method for measuring total oxidant status. Clin. Biochem., 38, 1103- 1111.
- Eyer P, Szinicz L, Thiermann H, Worek F, Zilker T (2007): Testing of antidotes for organophosphorus compounds: Experimental procedures and clinical reality. Toxicology, 233, 108-119.
- Gökalp O, Mollaoglu H, Yılmaz HR, Altuntas D (2003): Organofosfat insektisit fenthion’un rat amilaz ve lipaz enzimleri üzerine etkisi: Vitamin E ve C’nin rolü. Süley- man Demirel Üniv. Tıp Fak. Derg., 10, 21-23.
- Gülcü F, Gürsu MF (2003): Paraoksonaz ve aril esteraz aktivite ölçümlerinin standardizasyonu. Turk J. Biochem., 28, 45-49.
- Gültekin F, Oztürk M, Akdogan M (2000): The effect of organophosphate lipid peroxidation and antioxidant enzymes (in vitro). Arch. Toxicol., 74, 533–538.
- chlorpyrifos-ehtyl on
- Gültekin F, Delibaş N, Yaşar S, Kılınç I (2001): In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch. Toxicol., 75, 88-96.
- Harel M, Aharoni A, Gaidukov L, Brumshtein B, Khersonsky O, Meged R, Dvir H, Ravelli RBG, McCarthy A, Toker L, Silman I, Sussman JL, Tawfik DS (2004): Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nat. Struct. Mol. Biol., 11, 412-419.
- Ilhan A, Iraz M, Gürel A, Armutcu F, Akyol O (2004): Caffeic acid phenethyl ester exerts a neuroprotective effect on CNS against pentylenetetrazol-induced seizures in mice. Neurochem Res., 29, 2287-2292.
- Jacobsen H, Qstergaard G, Lam HR, Poulsen ME, Frandsen H, Ladefoged O, Meyer O (2004): Repeated dose 28-day oral toxicity study in Wistar rats with a mixture of five pesticides often found as residues in food: alphacypermethrin, chlorpyrifos and mancozeb. Food and Chem. Toxicol., 42, 1269-1277. carbendazim,
- Khan S, Khan, NN (1986): The mobility of some organophosphorus pesticides in soils as affected by some soil parameters. Soil Sci., 142, 214-222.
- Kösecik M, Erel O, Sevinc E, Selek S (2005): Increased oxidative stress in children exposed to passive smoking. Int J Cardiol., 100, 61-64.
- Li WF, Furlong C, Costa LG (1995): Paraoxonase protects against chlorpyrifos toxicity in mice. Toxicol. Lett., 76, 219-226.
- Lodovici M, Cassalini C, Briani C, Dolara P (1997): Oxidative liver DNA damage in rats treated with pesticide mixtures. Toxicology, 117, 55-60.
- Mitra NK, Nadarajah VD, Siong, HH (2009): Effect of concurrent application of heat, swim stres and repeated dermal application of chlorpyrifos on the hippocampal neurons in mice. Folia neuropath., 47, 60-68.
- Ncibi S, Othman MB, Akacha A, Krifi MN, Zourgui L (2008): Opuntia ficus indica extract protects against chlorpyrifos-induced damage on mice liver. Food and Chem Toxicol., 46, 797-802.
- Oncü M, Gültekin F, Karaöz E, Altuntaş I, Delibaş N (2002): Klorprifos-etil tarafından oluşturulan oksidatif ha- sarın sıçan karaciğerine etkileri. T Klin Tıp Bil. Derg., 22, 50-55.
- Peter JV, Moran JL, Graham P (2006): Oxime therapy and outcomes in human organophosphate poisoning: An evaluation using meta-analytic techniques. Crit. Care Med., 34, 502-510.
- Reuber MD (1978): Carcinomas and other lesions of the liver in mice ingestingorganochlorine pesticides. Clinical Toxicol., 13, 231- 256.
- Russo A, Longo R, Vanella A (2002): Antioxidant activity of propolis: Role of caffeic acid phenethyl ester and galangin. Fitoterapia, 73, 21-29.
- Stephen B, Kyle L, Yong X, Cynthia A, Donald E, Earl F, James E (1997): Role of oxidative stress in the mecha- nism of dieldrin's hepatotoxicity. Annals of Clin. and Lab. Sci., 27, 196-208.
- Timchalk C, Busby A, Campbell JA, Needham LL, Barr DN (2007): Comparative pharmacokinetics of the organophosphorus insecticide chlorpyrifos and its major metabolites diethylphosphate, diethylthiophosphate and 3,5,6-trichloro-2-pyridinol in the rat. Toxicology, 237, 145- 157.
- Uzun FG, Demir F, Kalender S, Baş H, Kalender Y (2010): Protective effect of catechin and quercetin on chlorpyrifos-induced lung toxicity in male rats. Food and Chem Toxicol., 48, 1714-1720.
- Watson AD, Berliner JA, Rama SY, La Du BN, Faul KF, Fogelman AM, Navab M (1995): Protective effect of high density lipoprotein associated Paraoxonase: Inhibition of the biological activity of minimally oxidized low density lipoprotein. J. Clin. Invest., 96, 2882-2891. 42. WHO:
- Organophosphorus Pesticides. Erişim
- (http://www.WHO.Org). Erişim Tarihi. 21.03.2013.
There are 44 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA43FM24PJ |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2015 |
| Published in Issue | Year 2015Volume: 62 Issue: 4 |