Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Türkiye’de etlik piliçlerde görülen Eimeria türlerinin moleküler olarak belirlenmesi

Year 2013, Volume: 60 Issue: 4, 245 - 250, 01.12.2013

Esin GÜVEN Robert B. BECKSTEAD Sırrı KAR Zati VATANSEVER Zafer KARAER

https://doi.org/10.1501/Vetfak_0000002587
Cited By: 7

Abstract

Bu çalışmada tavuklarda coccidiosise neden olan 7 Eimeria türünün Türkiye’deki varlığı araştırılmıştır. Çalışma materyali olan altlık ve dışkı örnekleri, Eylül 2006-Eylül 2007 tarihleri arasında, broiler yetiştiriciliği yapılan 817 çiftlikteki toplam 1110 kümesten toplandı. İncelenen örneklerin 624 (%56.2)’ünde Eimeria oocystleri saptandı. Genomik rDNA’nın internal transcribed spacer-1 (ITS-1) bölgesini hedefleyen klasik ve nested PCR yöntemleri 7 Eimeria türü için uygulandı. Klasik PCR yöntemi ile E. maxima, E. tenella, E. acervuline ve E. praecox türleri saptanırken E. mitis ve E. brunetti ancak nested PCR yöntemi ile belirlendi. Morfolojik ve morfometrik incelemelerde E. necatrix’e benzeyen oocystler gözlenmiş olmasına ragmen, her iki PCR yönteminde de bu etken belirlenmedi. PCR yöntemi ile belirlenen 6 Eimeria türüne ait nükleotid dizi bilgileri HQ680469-HQ680474 numaraları ile Genbank veri tabanına kaydedildi

Keywords

Broiler, Eimeria, klasik PCR, nested PCR

References

  • Aarthi S, Dhinakar Raj G, Raman M, Gomathinayagam S, Kumanan K (2010): Molecular prevalence and preponderance of Eimeria spp. among chickens in Tamil Nadu, India. Parasitol Res, 107, 1013- 1017.
  • Allen PC, Fetterer RH (2002): Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin Microbiol Rev, 15, 58-65.
  • Cantacessi C, Riddell S, Morris GM, Doran T, Woods WG, Otranto D, Gasser RB (2008): Genetic characterization of three unique operational taxonomic units of Eimeria from chickens in Australia based on nuclear spacer ribosomal DNA. Vet Parasitol, 152, 226- 34.
  • Carvalho FS, Wenceslau AA, Teixeira M, Matos Carneiro JA, Melo AD, Albuquerque GR (2011): Diagnosis of Eimeria species using traditional and molecular methods in field studies. Vet Parasitol, 176, 95-100.
  • Demir S (1992): Bursa bölgesi tavuklarında coccidiose etkenleri ve bunların yayılışı. Uludağ Univ Vet Fak Derg, 2, 143-151.
  • Gasser RB, Skinner R, Fadavi R, Richards G, Morris G (2005): High-throughput capillary electrophoresis for the identification and differentiation of seven species of Eimeria from chickens. Electrophoresis, 26, 3479-3485.
  • Haug A, Thebo P, Mattsson JG (2007): A simplified protocol for molecular identification of Eimeria species in field samples. Vet Parasitol, 146, 35-45.
  • Haug A, Gjevre A, Thebo P, Mattsson JG, Kaldhusdal M (2008): Coccidial infections in commercial broilers: Epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques. Avian Pathol, 37, 161-170.
  • Jordan FTW, Pattison M (1996) Poultry Diseases. W.B. Saunders Company Ltd, London.
  • Karaer Z, Guven E, Akcay A, Kar S, Nalbantoglu S, Cakmak A (2012): Prevalence of subclinical coccidiosis in broiler farms in Turkey. Trop Anim Health Pro, 44, 589- 594.
  • Karaş GD (2004): Etlik piliçlerde canlı aşı uygulama- larının koksidiyozdan korunmadaki etkisinin araştırılması. Unpublished doctoral dissertation. University of Ankara, Turkey.
  • Lee BH, Kim WH, Jeong J, Yoo J, Kwon YK, Jung BY, Kwon JH, Lillehoj HS, Min W (2010): Prevalence and cross-immunity of Eimeria species on Korean chicken farms. J Vet Med Sci, 72, 985-9.
  • Lew AE, Anderson GR, Minchin CM, Jeston PJ, Jorgensen WK (2003): Inter- and intra-strain variation and PCR detection of the internal transcribed spacer 1 (ITS-1) sequences of Australian isolates of Eimeria species from chickens. Vet Parasitol, 112, 33-50.
  • Lien YY, Sheu SC, Liu HJ, Chen SC, Tsai MY, Luo SC, Wu KC, Liu SS, Su HY (2007): Cloning and nucleotide sequencing of the second internal transcribed spacer of ribosomal DNA for three species of Eimeria from chickens in Taiwan. Vet J, 173, 186-191.
  • Long PL, Reid WM (1982): A guide for the diagnosis of coccidiosisin chickens. The University of Georgia, College of Agriculture Experiment Stations, Research Report 404, 1–17.
  • Long PL, Joyner LP (1984): Problems in the identification of species of Eimeria. J Protozool, 31, 535–541.
  • Mattiello R, Boviez JD, McDougald LR (2000): Eimeria brunetti and Eimeria necatrix in chickens of Argentina and confirmation of seven species of Eimeria. Avian Dis, 44, 711-714.
  • McDougald LR (2003): Protozoal infections. 973-1023. In: YM Saif, HJ Barnes, JR Glisson, AM Fadly, LR McDougald, DE Swayne (Eds), Diseases of Poultry. Iowa State Press, Ames.
  • Ministry of Agriculture, Fisheries and Food (1986): Enumeration of coccidial oocysts. 85-87. In: Manual of Veterinary Parasitological Laboratory Techniques, Reference Book 418. Her Majesty’s Stationary Office, London.
  • Morgan JA, Morris GM, Wlodek BM, Byrnes R, Jenner M, Constantinoiu CC, Anderson GR, Lew- Tabor AE, Molloy JB, Gasser RB, Jorgensen WK (2009): Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens. Mol Cell Probes, 23, 83-89.
  • Morris GM, Gasser RB (2006): Biotechnological advances in the diagnosis of avian coccidiosis and the analysis of genetic variation in Eimeria. Biotechnol Adv, 24, 590-603.
  • Ogedengbe JD, Hunter DB, Barta JR (2011): Molecular identification of Eimeria species infecting market-age meat chickens in commercial flocks in Ontario. Vet Parasitol, 178, 350-354.
  • Patra G, Ali MA, Chanu KV, Jonathan J, Joy LK, Prava M, Ravindran R, Das G, Devi LI (2010): PCR based diagnosis of Eimeria tenella infection in broiler chicken. Int J Poultry Sci, 9, 813-818.
  • Schnitzler BE, Thebo P, Mattson JG, Tomley F, Shirley MW (1998): Development of a diagnostic PCR assay for the detection anddiscrimination of four pathogenic Eimeria species of the chicken. Avian Pathol, 27, 490-497.
  • Schwarz RS, Jenkins MC, Klopp S, Miska KB (2009): Genomic analysis of Eimeria spp. populations in relation to performance levels of broiler chicken farms in Arkansas and North Carolina. J Parasitol, 95, 871-880.
  • Shirley MW, Smith AL, Tomley FM (2005): The biology of avian Eimeria with an emphasis on their control by vaccination. Adv Parasitol, 60, 285–330.
  • Sun XM, Pang W, Jia T, Yan WC, He G, Hao LL, Bentue´ M, Suo X (2009): Prevalence of Eimeria species in broilers with subclinical signs from fifty farms. Avian Dis, 53, 301-305.
  • Thebo P, Lunden A, Uggla A, Hooshmand-Rad P (1998): Identification of seven Eimeria species in Swedish domestic fowl. Avian Pathol, 27, 613-617.
  • Williams RB (1998): Epidemiological aspects of the use of live anticoccidial vaccines for chickens. Int J Parasitol, 28, 1089-1098.
  • Woods WG, Whithear KG, Richards DG, Anderson GR, Jorgensen WK, Gasser RB (2000): Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia. Int J Parasitol, 30, 1019-1023.
  • Zhang JJ, Wang LX, Ruan WK, An J (2013): Investigation into the prevalence of coccidiosis and maduramycin drug resistance in chickens in China. Vet Parasitol, 191, 29–34.

Molecular identification of Eimeria species of broiler chickens in Turkey

Year 2013, Volume: 60 Issue: 4, 245 - 250, 01.12.2013

Esin GÜVEN Robert B. BECKSTEAD Sırrı KAR Zati VATANSEVER Zafer KARAER

https://doi.org/10.1501/Vetfak_0000002587
Cited By: 7

Abstract

The objective of this study was to determine whether seven Eimeria species involved in chicken coccidiosis are present in Turkey and to assess their prevelance in commercial flocks. Litter and faecal samples were collected from 1110 broiler flocks housed in 817 farms (about 12% of all broiler farms in Turkey) between September 2006 and September 2007. Coccidian oocysts were found in 624 (56.2 %) of the samples examined. Species-specific polymerase chain reaction (PCR) and nested PCR tests targeting the internal transcribed spacer-1 (ITS-1) sequences of the genomic rDNA were performed for all seven Eimeria species. The results of species-specific PCR assays confirmed the presence of E. maxima, E. tenella, E. acervulina and E. praecox, and nested PCR results showed the presence of E. mitis and E. brunetti. Although morphologic and morphometric observations revealed the presence of oocysts resembling E. necatrix; it was not confirmed by species specific or nested PCR. Nucleotide sequences of Turkish Eimeria isolates obtained by sequencing of the PCR products from 6 Eimeria species have been entered into the GenBank sequence database under accession numbers HQ680469 through HQ680474

Keywords

Broiler, Eimeria species, species-specific PCR, nested PCR

References

  • Aarthi S, Dhinakar Raj G, Raman M, Gomathinayagam S, Kumanan K (2010): Molecular prevalence and preponderance of Eimeria spp. among chickens in Tamil Nadu, India. Parasitol Res, 107, 1013- 1017.
  • Allen PC, Fetterer RH (2002): Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin Microbiol Rev, 15, 58-65.
  • Cantacessi C, Riddell S, Morris GM, Doran T, Woods WG, Otranto D, Gasser RB (2008): Genetic characterization of three unique operational taxonomic units of Eimeria from chickens in Australia based on nuclear spacer ribosomal DNA. Vet Parasitol, 152, 226- 34.
  • Carvalho FS, Wenceslau AA, Teixeira M, Matos Carneiro JA, Melo AD, Albuquerque GR (2011): Diagnosis of Eimeria species using traditional and molecular methods in field studies. Vet Parasitol, 176, 95-100.
  • Demir S (1992): Bursa bölgesi tavuklarında coccidiose etkenleri ve bunların yayılışı. Uludağ Univ Vet Fak Derg, 2, 143-151.
  • Gasser RB, Skinner R, Fadavi R, Richards G, Morris G (2005): High-throughput capillary electrophoresis for the identification and differentiation of seven species of Eimeria from chickens. Electrophoresis, 26, 3479-3485.
  • Haug A, Thebo P, Mattsson JG (2007): A simplified protocol for molecular identification of Eimeria species in field samples. Vet Parasitol, 146, 35-45.
  • Haug A, Gjevre A, Thebo P, Mattsson JG, Kaldhusdal M (2008): Coccidial infections in commercial broilers: Epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques. Avian Pathol, 37, 161-170.
  • Jordan FTW, Pattison M (1996) Poultry Diseases. W.B. Saunders Company Ltd, London.
  • Karaer Z, Guven E, Akcay A, Kar S, Nalbantoglu S, Cakmak A (2012): Prevalence of subclinical coccidiosis in broiler farms in Turkey. Trop Anim Health Pro, 44, 589- 594.
  • Karaş GD (2004): Etlik piliçlerde canlı aşı uygulama- larının koksidiyozdan korunmadaki etkisinin araştırılması. Unpublished doctoral dissertation. University of Ankara, Turkey.
  • Lee BH, Kim WH, Jeong J, Yoo J, Kwon YK, Jung BY, Kwon JH, Lillehoj HS, Min W (2010): Prevalence and cross-immunity of Eimeria species on Korean chicken farms. J Vet Med Sci, 72, 985-9.
  • Lew AE, Anderson GR, Minchin CM, Jeston PJ, Jorgensen WK (2003): Inter- and intra-strain variation and PCR detection of the internal transcribed spacer 1 (ITS-1) sequences of Australian isolates of Eimeria species from chickens. Vet Parasitol, 112, 33-50.
  • Lien YY, Sheu SC, Liu HJ, Chen SC, Tsai MY, Luo SC, Wu KC, Liu SS, Su HY (2007): Cloning and nucleotide sequencing of the second internal transcribed spacer of ribosomal DNA for three species of Eimeria from chickens in Taiwan. Vet J, 173, 186-191.
  • Long PL, Reid WM (1982): A guide for the diagnosis of coccidiosisin chickens. The University of Georgia, College of Agriculture Experiment Stations, Research Report 404, 1–17.
  • Long PL, Joyner LP (1984): Problems in the identification of species of Eimeria. J Protozool, 31, 535–541.
  • Mattiello R, Boviez JD, McDougald LR (2000): Eimeria brunetti and Eimeria necatrix in chickens of Argentina and confirmation of seven species of Eimeria. Avian Dis, 44, 711-714.
  • McDougald LR (2003): Protozoal infections. 973-1023. In: YM Saif, HJ Barnes, JR Glisson, AM Fadly, LR McDougald, DE Swayne (Eds), Diseases of Poultry. Iowa State Press, Ames.
  • Ministry of Agriculture, Fisheries and Food (1986): Enumeration of coccidial oocysts. 85-87. In: Manual of Veterinary Parasitological Laboratory Techniques, Reference Book 418. Her Majesty’s Stationary Office, London.
  • Morgan JA, Morris GM, Wlodek BM, Byrnes R, Jenner M, Constantinoiu CC, Anderson GR, Lew- Tabor AE, Molloy JB, Gasser RB, Jorgensen WK (2009): Real-time polymerase chain reaction (PCR) assays for the specific detection and quantification of seven Eimeria species that cause coccidiosis in chickens. Mol Cell Probes, 23, 83-89.
  • Morris GM, Gasser RB (2006): Biotechnological advances in the diagnosis of avian coccidiosis and the analysis of genetic variation in Eimeria. Biotechnol Adv, 24, 590-603.
  • Ogedengbe JD, Hunter DB, Barta JR (2011): Molecular identification of Eimeria species infecting market-age meat chickens in commercial flocks in Ontario. Vet Parasitol, 178, 350-354.
  • Patra G, Ali MA, Chanu KV, Jonathan J, Joy LK, Prava M, Ravindran R, Das G, Devi LI (2010): PCR based diagnosis of Eimeria tenella infection in broiler chicken. Int J Poultry Sci, 9, 813-818.
  • Schnitzler BE, Thebo P, Mattson JG, Tomley F, Shirley MW (1998): Development of a diagnostic PCR assay for the detection anddiscrimination of four pathogenic Eimeria species of the chicken. Avian Pathol, 27, 490-497.
  • Schwarz RS, Jenkins MC, Klopp S, Miska KB (2009): Genomic analysis of Eimeria spp. populations in relation to performance levels of broiler chicken farms in Arkansas and North Carolina. J Parasitol, 95, 871-880.
  • Shirley MW, Smith AL, Tomley FM (2005): The biology of avian Eimeria with an emphasis on their control by vaccination. Adv Parasitol, 60, 285–330.
  • Sun XM, Pang W, Jia T, Yan WC, He G, Hao LL, Bentue´ M, Suo X (2009): Prevalence of Eimeria species in broilers with subclinical signs from fifty farms. Avian Dis, 53, 301-305.
  • Thebo P, Lunden A, Uggla A, Hooshmand-Rad P (1998): Identification of seven Eimeria species in Swedish domestic fowl. Avian Pathol, 27, 613-617.
  • Williams RB (1998): Epidemiological aspects of the use of live anticoccidial vaccines for chickens. Int J Parasitol, 28, 1089-1098.
  • Woods WG, Whithear KG, Richards DG, Anderson GR, Jorgensen WK, Gasser RB (2000): Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia. Int J Parasitol, 30, 1019-1023.
  • Zhang JJ, Wang LX, Ruan WK, An J (2013): Investigation into the prevalence of coccidiosis and maduramycin drug resistance in chickens in China. Vet Parasitol, 191, 29–34.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA56HB72AM
Journal Section Research Article
Authors

Esin GÜVEN
Department of Parasitology, Faculty of Veterinary Medicine, Ataturk University, Erzurum/Turkey;

Robert B. BECKSTEAD
Department of Parasitology, Faculty of Veterinary Medicine, Ataturk University, Erzurum/Turkey;

Sırrı KAR
Department of Parasitology, Faculty of Veterinary Medicine, Ataturk University, Erzurum/Turkey;

Zati VATANSEVER
Department of Parasitology, Faculty of Veterinary Medicine, Ataturk University, Erzurum/Turkey;

Zafer KARAER
Department of Parasitology, Faculty of Veterinary Medicine, Ataturk University, Erzurum/Turkey;

Publication Date December 1, 2013
Published in Issue Year 2013Volume: 60 Issue: 4

Cite

Bibtex @letter to editor { auvfd533253, journal = {Ankara Üniversitesi Veteriner Fakültesi Dergisi}, issn = {1300-0861}, eissn = {1308-2817}, address = {}, publisher = {Ankara University}, year = {2013}, volume = {60}, number = {4}, pages = {245 - 250}, doi = {10.1501/Vetfak\_0000002587}, title = {Molecular identification of Eimeria species of broiler chickens in Turkey}, key = {cite}, author = {Güven, Esin and Beckstead, Robert B. and Kar, Sırrı and Vatansever, Zati and Karaer, Zafer} }
APA Güven, E. , Beckstead, R. B. , Kar, S. , Vatansever, Z. & Karaer, Z. (2013). Molecular identification of Eimeria species of broiler chickens in Turkey . Ankara Üniversitesi Veteriner Fakültesi Dergisi , 60 (4) , 245-250 . DOI: 10.1501/Vetfak_0000002587
MLA Güven, E. , Beckstead, R. B. , Kar, S. , Vatansever, Z. , Karaer, Z. "Molecular identification of Eimeria species of broiler chickens in Turkey" . Ankara Üniversitesi Veteriner Fakültesi Dergisi 60 (2013 ): 245-250 <http://vetjournal.ankara.edu.tr/en/pub/issue/43575/533253>
Chicago Güven, E. , Beckstead, R. B. , Kar, S. , Vatansever, Z. , Karaer, Z. "Molecular identification of Eimeria species of broiler chickens in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 60 (2013 ): 245-250
RIS TY - JOUR T1 - Molecular identification of Eimeria species of broiler chickens in Turkey AU - EsinGüven, Robert B.Beckstead, SırrıKar, ZatiVatansever, ZaferKaraer Y1 - 2013 PY - 2013 N1 - doi: 10.1501/Vetfak_0000002587 DO - 10.1501/Vetfak_0000002587 T2 - Ankara Üniversitesi Veteriner Fakültesi Dergisi JF - Journal JO - JOR SP - 245 EP - 250 VL - 60 IS - 4 SN - 1300-0861-1308-2817 M3 - doi: 10.1501/Vetfak_0000002587 UR - https://doi.org/10.1501/Vetfak_0000002587 Y2 - 2023 ER -
EndNote %0 Ankara Üniversitesi Veteriner Fakültesi Dergisi Molecular identification of Eimeria species of broiler chickens in Turkey %A Esin Güven , Robert B. Beckstead , Sırrı Kar , Zati Vatansever , Zafer Karaer %T Molecular identification of Eimeria species of broiler chickens in Turkey %D 2013 %J Ankara Üniversitesi Veteriner Fakültesi Dergisi %P 1300-0861-1308-2817 %V 60 %N 4 %R doi: 10.1501/Vetfak_0000002587 %U 10.1501/Vetfak_0000002587
ISNAD Güven, Esin , Beckstead, Robert B. , Kar, Sırrı , Vatansever, Zati , Karaer, Zafer . "Molecular identification of Eimeria species of broiler chickens in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 60 / 4 (December 2013): 245-250 . https://doi.org/10.1501/Vetfak_0000002587
AMA Güven E. , Beckstead R. B. , Kar S. , Vatansever Z. , Karaer Z. Molecular identification of Eimeria species of broiler chickens in Turkey. Ankara Univ Vet Fak Derg. 2013; 60(4): 245-250.
Vancouver Güven E. , Beckstead R. B. , Kar S. , Vatansever Z. , Karaer Z. Molecular identification of Eimeria species of broiler chickens in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2013; 60(4): 245-250.
IEEE E. Güven , R. B. Beckstead , S. Kar , Z. Vatansever and Z. Karaer , "Molecular identification of Eimeria species of broiler chickens in Turkey", Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 60, no. 4, pp. 245-250, Dec. 2013, doi:10.1501/Vetfak_0000002587

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