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Arthritisli ishalli buzağılarda eklem sıvısı proteinlerinin SDS-PAGE ile elektroforetik analizi

Year 2012, Volume: 59 Issue: 3, 169 - 174, 01.09.2012

Arif Altıntaş Hamdi Uysal Ulvi R. Fidancı Ömer Beşaltı Mert Pekcan

https://doi.org/10.1501/Vetfak_0000002521

Abstract

Çalışmada, klinik olarak sağlıklı (kontrol) ve ishalli buzağılara ait arthritisli eklem sıvısı örneklerinde proteinlerin elektroforetik olarak incelenmesi (SDS-PAGE) amaçlanmıştır. Çalışmada 3-29 günlük sağlıklı 7 buzağı (kontrol) ile arthritis gözlenen 19 ishalli buzağıdan (2-75 günlük) materyal olarak yararlanılmıştır. Eklem sıvısı proteinlerinin molekül ağırlıklarına göre ayırımı SDS-PAGE (8x10 cm mini-jelde %10 SDS içeren %7.5 akrilamid) ile gerçekleştirilmiştir. SDS-PAGE sonrası sağlıklı ve arthritisli eklem sıvılarında 48-50 kDa ve 56-57 kDa temel bandlar olarak gözlenmiştir. İki grup arasında fark yaratan 18 protein bandı dikkat çekici bulunmuştur. Sonuç olarak; eklem sıvısında 205.5: 200-190; 144-140; 110-100; 64-62; 39-40 ve muhtemelen ≤10 kDa protein bandlarının tek tek ya da birlikte ishalli buzağılarda arthritisin şiddetini belirleyici bir gösterge olabileceği ve protein bandlarda gözlenen değişikliklerin ishalli buzağılarda eklem bozukluğunun patofizyolojisinde rol oynayan etyolojik faktörleri anlamada yardımcı olabileceği, bu nedenle de Veteriner klinikte tanısal ve prognostik önemi olabileceği kanısına varılmıştır

Keywords

Arthritis buzağı ishali eklem sıvısı SDS-PAGE sinovyal protein

References

  • Altıntaş A, Karagül H, Fidancı UR, Uysal H, Beşaltı Ö, Pekcan M,Aypak SÜ, Çiftci G, Bilgihan S, Hanedan B (2010). Hyaluronic Acid Levels and Physical Characteristics of Synovial Fluid in Healthy and Diarrheic Calves with Arthritis. Turkish Journal of Biochemistry, 35 (1): 14 - 19.
  • Anonim (-). http://www.physiological values for all species. Erişim Tarihi: 19.02.2007.
  • Anonim (2007): Comprehensıve Lıst Of Procedures. http://www.labcorp.com/datasets/labcorp/html/chapter/mo no/pr009500.htm. Copyright © 2007 by Laboratory Corporation of America® Holdings and Lexi-Comp Inc.
  • Bailey MJ, Lacey DC, de Kok BV, Veith PD, Reynolds EC, Hamilton JA (2010): Extracellular proteomes of M- CSF (CSF-1) and GM-CSF-dependent macrophages. Immunol Cell Biol. Jul 27 (E-basım).
  • Brenner J, Elad D, Markovics A and Trainin Z (1993): Epidemiological study of neonatal calf diarrhoea in İsrael- A one year survey of fecal samples. Isr. J. Vet. Med. 48: 113-116.
  • Brenner J, David D, Bernstein M and Elad D. (1998). Description of an episode of multifocal polyarthritis of unknown aetiology in Newborn Calves. J. Vet. Med. B, 52:(2) 98.
  • Cherniakova IuM, Pinchuk LS (2009): Molecular mass distribution of proteins in serum and synovial fluid. Klin Lab Diagn. 12: 8-11.
  • Fujimura K, Segami N, Yoshitake Y, Tsuruoka N, Kaneyama K, Sato J, Kobayashi S (2005): Electrophoretic separation of the synovial fluid proteins in patients with temporomandibular joint disorders. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Apr;101(4):463-8. Epub 2005 Oct 14.
  • Gibson DS, Finnegan S, Jordan G, Scaife C, Brockbank S, Curry J, McAllister C, Pennington S, Dunn M, Rooney ME (2009): Stratification and monitoring of juvenile idiopathic arthritis patients by synovial proteome analysis. J Proteome Res. 8 (12):5601-9.
  • González-Buitrago JM, Ferreira L, Muñiz MC (2008): Clinical proteomics and new biomarkers in biological fluids. Med Clin (Barc). 4, 131 (11):426-34.
  • Hain NA, Stuhlmuller B, Hahn GR, Kalden JR, Deutzmann R and Burmester GR (1996): Biochemical characterization and microsequencing of a 205-kDa synovial protein stimulatory for T cells and reactive with rheumatoid factor containing sera. The Journal of Immunology, 157 (4): 1773-1780.
  • Harvey S, Weisman M, O'Dell J, Scott T, Krusemeier M, Visor J, Swindlehurst C (1998): Enzymes and Protein Markers. Chondrex: new marker of joint disease. Clinical Chemistry. 1998; 44:509-516.
  • Hewicker-Trautwein M, Peters M, Gruber A, Baum B, Liverköhne I, Buchenau I, Kleinschmidt S (2003): Bronchopneumonia and polyarthritis due to Mycoplasma bovis in a calf. Dtsch Tierarztl Wochenschr. 110 (4):147-150.
  • HSI (1993): Hoeffer Scientific Instruments. Hoeffer Protein electrophoresis Applications Guide. San Francisco USA.
  • Ježek J, Klopčıč M and Klınkon M (2006). Influence of age on biochemical parameters in calves. Bull Vet Inst Pulawy 50, 211-214.
  • Karagül H, Altıntaş A, Fidancı UR, Sel T (2000): Klinik Biyokimya. Medisan Yayınevi. Yayın Serisi: 45. 1. Baskı- Ankara.
  • Kinloch A, Lundberg K, Wait R, Wegner N, Lim NH, Zendman AJ, Saxne T, Malmström V, Venables PJ (2008): Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis. Arthritis Rheum. 58 (8): 2287-2295.
  • Li TW, Zheng BR, Huang ZX, Lin Q, Zhao LK, Liao ZT, Zhao JJ, Lin ZM, Gu JR (2010): Screening disease- associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study. Chin Med J (Engl). 5; 123 (5): 537-543.
  • Lipowitz AJ (1985): Synovial Fluid. Alınmıştır: Textbook of Small Animal Orthopaedics, CD Newton and DM Nunamaker (Eds). 1985.
  • Nyırkos P and Golds E.E (1990): Human synovial cells secrete a 39 kDa protein similar to a bovine mammary protein expressed during the non-lactating period. Biochem J. 268, 265-268.
  • Rosenkranz ME, Wilson DC, Marinov AD, Decewicz A, Grof-Tisza P, Kirchner D, Giles B, Reynolds PR, Liebman MN, Kolli VS, Thompson SD, Hirsch R (2010): Synovial fluid proteins differentiate between the subtypes of juvenile idiopathic arthritis. Arthritis Rheum. 62 (6): 1813-23.
  • Smith BP (1996): Large Animal Internal Medicine. Diseases of Horses, Cattle, Sheep and Goats. Second edition. pp. 403, St. Louis, Mosby-Year Book, Inc.
  • Svensson C, Jensen MB (2007): Short communication: Identification of diseased calves by use of data from automatic milk feeders. J Dairy Sci. 90 (2): 994-997.
  • Şahal M, Kurtdede A, Börkü K, Ünsüren H, İmren HY, Özlem MB, Kalınbacak A (1994): Yeni doğan ishalli buzağıların klinik bulguları ve asit baz dengesi dikkate alınarak sodyum bikarbonat ve elektrolitik sıvılarla sağaltımı. Ankara Üniv Vet Fak Derg, 41: 599-625.
  • Uchida T, Fukawa A, Uchida M, Fujita K, and Saito K (2002): Application of a Novel Protein Biochip Technology for detection and identification of Rheumatoid Arthritis Biomarkers in Synovial Fluid. Journal of Proteome Research, 1 (6): 495-499.
  • Yamagiwa H, Sarkar G, Charlesworth MC, McCormick DJ, Bolander ME (2003): Two-dimensional gel electrophoresis of synovial fluid: method for detecting candidate protein markers for osteoarthritis. J Orthop Sci, 8 (4): 482-490. Geliş tarihi: 25.01.2011 / Kabul tarihi: 20.01.2012 Yazışma Prof. Dr. Arif Altıntaş Ankara Üniverstesi Veteriner Fakültesi Biyokimya AD, 06110 Ankara E-posta: altintas@veterinary.ankara.edu.tr

Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves

Year 2012, Volume: 59 Issue: 3, 169 - 174, 01.09.2012

Arif Altıntaş Hamdi Uysal Ulvi R. Fidancı Ömer Beşaltı Mert Pekcan

https://doi.org/10.1501/Vetfak_0000002521

Abstract

In the study, synovial fluid samples obtained from clinically healthy calves (control) and diarrheic calves with arthritis were examined by electrophoresis (SDS-PAGE) to determine the distribution of denatured proteins for understanding their clinical importance. In the study, 3-29 days old 7 healthy calves (control) and 19 diarrheaic calves observed in arthritis (2-75 days old) were used as material. Synovial fluid proteins were separated by SDS-PAGE (7.5% + 10% SDS-containing acrylamide, on 8x10 cm mini-gel) according to the molecular weights. Protein bands with the molecular weight of 48-50 kDa and 56-57 kDa were the main bands after SDS-PAGE analysis of joint fluids of calves with and without arthritis. Eighteen protein bands were dominantly found in arthritic group. As a result, joint fluid protein bands with the molecular weights of 205.5; 200-190; 144-140; 110-100; 6462; 40-39 and <10 kDa may be important together or individually to determine the degree of severity of arthritis in calves. Moreover, these changes in protein bands may also be useful to understand the ethiologic factors in the pathophysiology of arthritis. Therefore, obtained results may have a diagnostic and prognostic value in the veterinary clinics

Keywords

Arthritis calf diarrhea SDS-PAGE synovial fluid synovial protein

References

  • Altıntaş A, Karagül H, Fidancı UR, Uysal H, Beşaltı Ö, Pekcan M,Aypak SÜ, Çiftci G, Bilgihan S, Hanedan B (2010). Hyaluronic Acid Levels and Physical Characteristics of Synovial Fluid in Healthy and Diarrheic Calves with Arthritis. Turkish Journal of Biochemistry, 35 (1): 14 - 19.
  • Anonim (-). http://www.physiological values for all species. Erişim Tarihi: 19.02.2007.
  • Anonim (2007): Comprehensıve Lıst Of Procedures. http://www.labcorp.com/datasets/labcorp/html/chapter/mo no/pr009500.htm. Copyright © 2007 by Laboratory Corporation of America® Holdings and Lexi-Comp Inc.
  • Bailey MJ, Lacey DC, de Kok BV, Veith PD, Reynolds EC, Hamilton JA (2010): Extracellular proteomes of M- CSF (CSF-1) and GM-CSF-dependent macrophages. Immunol Cell Biol. Jul 27 (E-basım).
  • Brenner J, Elad D, Markovics A and Trainin Z (1993): Epidemiological study of neonatal calf diarrhoea in İsrael- A one year survey of fecal samples. Isr. J. Vet. Med. 48: 113-116.
  • Brenner J, David D, Bernstein M and Elad D. (1998). Description of an episode of multifocal polyarthritis of unknown aetiology in Newborn Calves. J. Vet. Med. B, 52:(2) 98.
  • Cherniakova IuM, Pinchuk LS (2009): Molecular mass distribution of proteins in serum and synovial fluid. Klin Lab Diagn. 12: 8-11.
  • Fujimura K, Segami N, Yoshitake Y, Tsuruoka N, Kaneyama K, Sato J, Kobayashi S (2005): Electrophoretic separation of the synovial fluid proteins in patients with temporomandibular joint disorders. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Apr;101(4):463-8. Epub 2005 Oct 14.
  • Gibson DS, Finnegan S, Jordan G, Scaife C, Brockbank S, Curry J, McAllister C, Pennington S, Dunn M, Rooney ME (2009): Stratification and monitoring of juvenile idiopathic arthritis patients by synovial proteome analysis. J Proteome Res. 8 (12):5601-9.
  • González-Buitrago JM, Ferreira L, Muñiz MC (2008): Clinical proteomics and new biomarkers in biological fluids. Med Clin (Barc). 4, 131 (11):426-34.
  • Hain NA, Stuhlmuller B, Hahn GR, Kalden JR, Deutzmann R and Burmester GR (1996): Biochemical characterization and microsequencing of a 205-kDa synovial protein stimulatory for T cells and reactive with rheumatoid factor containing sera. The Journal of Immunology, 157 (4): 1773-1780.
  • Harvey S, Weisman M, O'Dell J, Scott T, Krusemeier M, Visor J, Swindlehurst C (1998): Enzymes and Protein Markers. Chondrex: new marker of joint disease. Clinical Chemistry. 1998; 44:509-516.
  • Hewicker-Trautwein M, Peters M, Gruber A, Baum B, Liverköhne I, Buchenau I, Kleinschmidt S (2003): Bronchopneumonia and polyarthritis due to Mycoplasma bovis in a calf. Dtsch Tierarztl Wochenschr. 110 (4):147-150.
  • HSI (1993): Hoeffer Scientific Instruments. Hoeffer Protein electrophoresis Applications Guide. San Francisco USA.
  • Ježek J, Klopčıč M and Klınkon M (2006). Influence of age on biochemical parameters in calves. Bull Vet Inst Pulawy 50, 211-214.
  • Karagül H, Altıntaş A, Fidancı UR, Sel T (2000): Klinik Biyokimya. Medisan Yayınevi. Yayın Serisi: 45. 1. Baskı- Ankara.
  • Kinloch A, Lundberg K, Wait R, Wegner N, Lim NH, Zendman AJ, Saxne T, Malmström V, Venables PJ (2008): Synovial fluid is a site of citrullination of autoantigens in inflammatory arthritis. Arthritis Rheum. 58 (8): 2287-2295.
  • Li TW, Zheng BR, Huang ZX, Lin Q, Zhao LK, Liao ZT, Zhao JJ, Lin ZM, Gu JR (2010): Screening disease- associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study. Chin Med J (Engl). 5; 123 (5): 537-543.
  • Lipowitz AJ (1985): Synovial Fluid. Alınmıştır: Textbook of Small Animal Orthopaedics, CD Newton and DM Nunamaker (Eds). 1985.
  • Nyırkos P and Golds E.E (1990): Human synovial cells secrete a 39 kDa protein similar to a bovine mammary protein expressed during the non-lactating period. Biochem J. 268, 265-268.
  • Rosenkranz ME, Wilson DC, Marinov AD, Decewicz A, Grof-Tisza P, Kirchner D, Giles B, Reynolds PR, Liebman MN, Kolli VS, Thompson SD, Hirsch R (2010): Synovial fluid proteins differentiate between the subtypes of juvenile idiopathic arthritis. Arthritis Rheum. 62 (6): 1813-23.
  • Smith BP (1996): Large Animal Internal Medicine. Diseases of Horses, Cattle, Sheep and Goats. Second edition. pp. 403, St. Louis, Mosby-Year Book, Inc.
  • Svensson C, Jensen MB (2007): Short communication: Identification of diseased calves by use of data from automatic milk feeders. J Dairy Sci. 90 (2): 994-997.
  • Şahal M, Kurtdede A, Börkü K, Ünsüren H, İmren HY, Özlem MB, Kalınbacak A (1994): Yeni doğan ishalli buzağıların klinik bulguları ve asit baz dengesi dikkate alınarak sodyum bikarbonat ve elektrolitik sıvılarla sağaltımı. Ankara Üniv Vet Fak Derg, 41: 599-625.
  • Uchida T, Fukawa A, Uchida M, Fujita K, and Saito K (2002): Application of a Novel Protein Biochip Technology for detection and identification of Rheumatoid Arthritis Biomarkers in Synovial Fluid. Journal of Proteome Research, 1 (6): 495-499.
  • Yamagiwa H, Sarkar G, Charlesworth MC, McCormick DJ, Bolander ME (2003): Two-dimensional gel electrophoresis of synovial fluid: method for detecting candidate protein markers for osteoarthritis. J Orthop Sci, 8 (4): 482-490. Geliş tarihi: 25.01.2011 / Kabul tarihi: 20.01.2012 Yazışma Prof. Dr. Arif Altıntaş Ankara Üniverstesi Veteriner Fakültesi Biyokimya AD, 06110 Ankara E-posta: altintas@veterinary.ankara.edu.tr
There are 26 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA64EG36US
Journal Section Research Article
Authors

Arif Altıntaş

Hamdi Uysal

Ulvi R. Fidancı

Ömer Beşaltı

Mert Pekcan

Publication Date September 1, 2012
Published in Issue Year 2012Volume: 59 Issue: 3

Cite

APA Altıntaş, A., Uysal, H., Fidancı, U. R., Beşaltı, Ö., et al. (2012). Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 59(3), 169-174. https://doi.org/10.1501/Vetfak_0000002521
AMA Altıntaş A, Uysal H, Fidancı UR, Beşaltı Ö, Pekcan M. Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves. Ankara Univ Vet Fak Derg. September 2012;59(3):169-174. doi:10.1501/Vetfak_0000002521
Chicago Altıntaş, Arif, Hamdi Uysal, Ulvi R. Fidancı, Ömer Beşaltı, and Mert Pekcan. “Electrophoretic Analysis of Arthritic Synovial Fluid Proteins by SDS-PAGE in Diarrheic Calves”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 59, no. 3 (September 2012): 169-74. https://doi.org/10.1501/Vetfak_0000002521.
EndNote Altıntaş A, Uysal H, Fidancı UR, Beşaltı Ö, Pekcan M (September 1, 2012) Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves. Ankara Üniversitesi Veteriner Fakültesi Dergisi 59 3 169–174.
IEEE A. Altıntaş, H. Uysal, U. R. Fidancı, Ö. Beşaltı, and M. Pekcan, “Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves”, Ankara Univ Vet Fak Derg, vol. 59, no. 3, pp. 169–174, 2012, doi: 10.1501/Vetfak_0000002521.
ISNAD Altıntaş, Arif et al. “Electrophoretic Analysis of Arthritic Synovial Fluid Proteins by SDS-PAGE in Diarrheic Calves”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 59/3 (September 2012), 169-174. https://doi.org/10.1501/Vetfak_0000002521.
JAMA Altıntaş A, Uysal H, Fidancı UR, Beşaltı Ö, Pekcan M. Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves. Ankara Univ Vet Fak Derg. 2012;59:169–174.
MLA Altıntaş, Arif et al. “Electrophoretic Analysis of Arthritic Synovial Fluid Proteins by SDS-PAGE in Diarrheic Calves”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 59, no. 3, 2012, pp. 169-74, doi:10.1501/Vetfak_0000002521.
Vancouver Altıntaş A, Uysal H, Fidancı UR, Beşaltı Ö, Pekcan M. Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves. Ankara Univ Vet Fak Derg. 2012;59(3):169-74.