Abstract
Bu çalışmada, Yeni Zelanda Beyazı 6-8 haftalık tavşanlara intranazal, intratraheal ve intravenöz olarak tavşan orijinli Pasteurella multocida 3:A serotipi inoküle edildi. Hayvanlar inokülasyon sonrası 4-6, 8-10 ve 14. günlerde öldü ya da ötanazi uygulandı. Tüm dokular makroskobik ve mikroskobik olarak muayene edildi. Bunlara ilaveten, akciğer lezyonları elektron mikroskobik olarak incelendi. Akciğer lezyonları en fazla intratraheal inokülasyon grubunda, daha az olarak intranazal inokülasyon grubunda ve en az olarak da intravenöz olarak inoküle edilen tavşanlarda görüldü. Pasteurella multocida 3:A serotipi inoküle edilmiş tüm gruplarda özellikle aşağı solunum sistemi lezyonları şekillendi. Akciğer ve plöra lezyonları intranazal olarak inoküle edilmiş hayvanların %50’sinde, intratraheal olarak inoküle edilmiş hayvanların %75’inde ve intravenöz olarak inoküle edilmiş hayvanların ise %17’sinde görüldü. Tavşanlarda histolojik olarak şiddetli fibrinopurulent ve nekrotik pnömoni, fibrinopurulent plöritis ve fibrinopurulent perikarditise rastlandı. Pasteurella multocida’lara elektron mikroskobik olarak serbest halde alveol boşluklarında ve fagosite edilmiş halde kapillar lumenlerindeki ve alveol boşluklarındaki nötrofil granülositlerde rastlandı. Sonuç olarak, Pasteurella multocida 3:A serotipi tavşanlara hangi yolla inoküle edilirse edilsin pnömoni oluşturulabildiği fakat en etkili yolun intratraheal yol olduğu, bunu sırası ile intranazal ve intravenöz yolun izlediği belirlendi
References
- Alexander MM, Sawin PB, Roehm DA (1952): Respiratory infection in the rabbit: An enzootie caused by Pasteurella lepiseptica and attempts to control it by vaccination. J Infect Dis, 90, 30-33.
- Al-Haddawi MH, Jasni S, Israf DA, Zamri-Saad M, Mutalib AR, Sheikh-Omar AR (2001): Ultrastructural pathology of nasal and tracheal mucosa of rabbits experimentally infected with Pasteurella multocida serotype D:1. Res Vet Sci, 70, 191-197.
- Al-Haddawi MH, Jasni S, Zamri-Saad M, Mutalib AR, Son R, Sheikh-Omar AR (2000a): Ultrastructural observation of nasal and pulmonary intracellular Pasteurella multocida A:3 in rabbits. Vet Res Commun, 24, 153-167.
- Al-Haddawi MH, Jasni S, Zamri-Saad M, Mutalib AR, Zulkifli I, Son R, Sheikh-Omar AR (2000b): In vitro study of Pasteurella multocida adhesion to trachea, lung and aorta of rabbits. Vet J, 159, 274-281.
- DeLong D, Manning PJ (1994): Bacterial disease, pp. 131-170. In Manning PJ, Ringler DH, Newcomer CE The Biology of Laboratory Rabbit. 2nd edition. Academic Press, Inc., Orlando, Fla.
- Dillehay DL, Paul KS, Digiacomo RF, Chengappa MM (1990): Pathogenicity of Pasteurella multocida A:3 in Flemish Giant and New Zealand White rabbits. Lab Anim, 25, 337-341.
- Flatt RE, Dungworth DL (1971a): Enzootic pneumonia in rabbits: Naturally occurring lesions in lungs of apparently healthy young rabbits. Am J Vet Res, 32, 321- 326.
- Flatt RE, Dungworth DL (1971b): Enzootic pneumonia in rabbits: Microbiology and comparison with lesions experimentally produced by Pasteurella multocida and a chlamydial organism. Am J Vet Res, 32, 627-637.
- Glavits R, Magyar T (1990): The pathology of respiratory infection with Pasteurella multocida and Bordetella bronchiseptica in rabbits. Acta Vet Hung, 38, 211-215.
- Hagen KW (1958): Enzootic pasteurellosis in domestic rabbits. I. Pathology and bacteriology. J Am Vet Med Assoc, 133, 77-80.
- Ostler DC (1961): The disease of broiler rabbits. Vet Rec, 73, 1237-1253.
- Pace LW, Turk JR, Corstvet RE, Enright FM, Henk W (1994): Induction of acut bronchopneumonia in mice by intrabronchial inoculation of Pasteurella haemolytica serotype1. Can J Vet Res, 58, 79-82.
- Percy DH, Bhasin JL, Rosendal S (1986): Experimental pneumonia in rabbits inoculated with strains of Pasteurella multocida. Can J Vet Res, 50, 36-41.
- Redondo E, Roncero V, Masot AJ, Duran E, Gazquez A (1990): Experimental rabbit pneumonic pasteurellosis: an ultrastructural and morphometric study. Acta Med Vet, 36, 3-20.
- Redondo E, Masot AJ, Gázquez A, Roncero V, Durán E, Piriz S (1993): Experimental reproduction of acute pneumonic pasteurellosis in rabbits. Histol Histopathol, 8, 97-104.
- Sokkar SM, Mohammed MA, Fetaih H (1987). Pathogenesis of Pasteurella multocida in experimentally infected rabbits. Arch Exp Vet Med, 41, 516-521.
- Webster LT (1925): Epidemiological studies on respiratory infections of the rabbit. VII. Pneumonia associated with Bacterium lepisepticum. J Exp Med, 43, 555-572.
- Whiteley LO, Maheswaran SK, Weiss DJ, Ames TR (1991): Alteration in pulmonary morphology and peripheral coagulation profiles caused by intratracheal inoculation of live and ultraviolet light-killed Pasteurella haemolytica A1 in calves. Vet Pathol, 28, 275-285.
Abstract
In this study, three groups of New Zealand White rabbits, 6-8-week-old, were inoculated with Pasteurella multocida serotype 3:A strain of rabbit origin by routes of intranasal, intratracheal and intravenous inoculations, respectively. The animals died or killed after the 4-6, 8-10 and 14 days of observation periods were necropsied. All of tissues were examined macroscopically and microscopically. In addition, pulmonary lesions were examined with electron microscopically. Pneumonic lesions were detected most consistently in rabbits inoculated intratracheally. Intranasal inoculation was less effective than intratracheal inoculation. The lesions were minimal in intravenous dosed group. All of the routes of Pasteurella multocida serotype 3:A inoculations produced especially the lower respiratory tract lesions. Pulmonary lesions were observed in %50 of animal inoculated intranasally, %75 of animals inoculated intratracheally and %17 of animals inoculated intravenously. The histological examinations revealed severe fibrinopurulent and necrotic pneumoni, fibrinopurulent pleuritis and fibrinopurulent pericarditis. Pasteurella multocida were shown electron microscopically in free manner in alveolar spaces, in phagocytic vacuoles of cytoplasm of neutrophilic granulocytes in alveolar spaces and in alveolar capillary lumens. As a result, Pasteurella multocida 3:A serotype can induce pneumonia in rabbits through different routes of inoculation: intratracheal, intranasal, and intravenous. However, intratracheal inoculation is the most effective method to induce pneumonia while intranasal inoculation is the second most and intravenous inoculation is the least effective
References
- Alexander MM, Sawin PB, Roehm DA (1952): Respiratory infection in the rabbit: An enzootie caused by Pasteurella lepiseptica and attempts to control it by vaccination. J Infect Dis, 90, 30-33.
- Al-Haddawi MH, Jasni S, Israf DA, Zamri-Saad M, Mutalib AR, Sheikh-Omar AR (2001): Ultrastructural pathology of nasal and tracheal mucosa of rabbits experimentally infected with Pasteurella multocida serotype D:1. Res Vet Sci, 70, 191-197.
- Al-Haddawi MH, Jasni S, Zamri-Saad M, Mutalib AR, Son R, Sheikh-Omar AR (2000a): Ultrastructural observation of nasal and pulmonary intracellular Pasteurella multocida A:3 in rabbits. Vet Res Commun, 24, 153-167.
- Al-Haddawi MH, Jasni S, Zamri-Saad M, Mutalib AR, Zulkifli I, Son R, Sheikh-Omar AR (2000b): In vitro study of Pasteurella multocida adhesion to trachea, lung and aorta of rabbits. Vet J, 159, 274-281.
- DeLong D, Manning PJ (1994): Bacterial disease, pp. 131-170. In Manning PJ, Ringler DH, Newcomer CE The Biology of Laboratory Rabbit. 2nd edition. Academic Press, Inc., Orlando, Fla.
- Dillehay DL, Paul KS, Digiacomo RF, Chengappa MM (1990): Pathogenicity of Pasteurella multocida A:3 in Flemish Giant and New Zealand White rabbits. Lab Anim, 25, 337-341.
- Flatt RE, Dungworth DL (1971a): Enzootic pneumonia in rabbits: Naturally occurring lesions in lungs of apparently healthy young rabbits. Am J Vet Res, 32, 321- 326.
- Flatt RE, Dungworth DL (1971b): Enzootic pneumonia in rabbits: Microbiology and comparison with lesions experimentally produced by Pasteurella multocida and a chlamydial organism. Am J Vet Res, 32, 627-637.
- Glavits R, Magyar T (1990): The pathology of respiratory infection with Pasteurella multocida and Bordetella bronchiseptica in rabbits. Acta Vet Hung, 38, 211-215.
- Hagen KW (1958): Enzootic pasteurellosis in domestic rabbits. I. Pathology and bacteriology. J Am Vet Med Assoc, 133, 77-80.
- Ostler DC (1961): The disease of broiler rabbits. Vet Rec, 73, 1237-1253.
- Pace LW, Turk JR, Corstvet RE, Enright FM, Henk W (1994): Induction of acut bronchopneumonia in mice by intrabronchial inoculation of Pasteurella haemolytica serotype1. Can J Vet Res, 58, 79-82.
- Percy DH, Bhasin JL, Rosendal S (1986): Experimental pneumonia in rabbits inoculated with strains of Pasteurella multocida. Can J Vet Res, 50, 36-41.
- Redondo E, Roncero V, Masot AJ, Duran E, Gazquez A (1990): Experimental rabbit pneumonic pasteurellosis: an ultrastructural and morphometric study. Acta Med Vet, 36, 3-20.
- Redondo E, Masot AJ, Gázquez A, Roncero V, Durán E, Piriz S (1993): Experimental reproduction of acute pneumonic pasteurellosis in rabbits. Histol Histopathol, 8, 97-104.
- Sokkar SM, Mohammed MA, Fetaih H (1987). Pathogenesis of Pasteurella multocida in experimentally infected rabbits. Arch Exp Vet Med, 41, 516-521.
- Webster LT (1925): Epidemiological studies on respiratory infections of the rabbit. VII. Pneumonia associated with Bacterium lepisepticum. J Exp Med, 43, 555-572.
- Whiteley LO, Maheswaran SK, Weiss DJ, Ames TR (1991): Alteration in pulmonary morphology and peripheral coagulation profiles caused by intratracheal inoculation of live and ultraviolet light-killed Pasteurella haemolytica A1 in calves. Vet Pathol, 28, 275-285.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA37ZB32BA |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2011 |
| Published in Issue | Year 2011Volume: 58 Issue: 1 |