Abstract
Capsaicin (CAP) is pungent ingredient in red peppers. On the ovary, the effects of high dose capsaicin are wellestablished, although the effects of low doses are unknown. The goal of the present study was to investigate the effects of low dose of CAP, equal to getting from hot pepper in daily diet, on the expression of X-linked Inhibitor of Apoptosis Protein (XIAP) and transcription factor Nuclear Factor kappa B (NF-kB) proteins having important roles on ovarian follicular development. In this study, 80 immature female Spraque-Dawley rats (21 days old) were used. The rats were randomly allocated into 4 groups according to injection periods as followed 6, 9, 12, 15 days. At the end of the experiment, tissue samples were collected and ovary sections were stained with streptavidin-biotin technique using primary antibodies against XIAP and NF-kB. As a result, while positive XIAP and NF-kB immunreactivity were observed in all healthy follicle stages in the ovary, no staining was observed in the granulosa and theca cells of the atretic follicles. Moreover, interstitial cells, ovarian surface epithelial cells and oocytes were also positively stained with XIAP and NF-kB. It was observed that the expression of XIAP and NF-kB protein were dependent on each other and particularly in 15 days injection group, these protein expressions in the experiment groups were significantly higher than both in control A and control B groups. As a result, short term-low dose CAP treatment induced XIAP expression via NF-kB activation and protected follicles from atresia. Consequently, it was thought that the low dose capsaicin may be one of the factors arranging the follicle development
Keywords
References
- Asselin E, Mills GB, Tsang BK (2001): XIAP regulates Akt activity and caspase-3-dependent cleavage during cisplatin-induced apoptosis in human ovarian epithelial cancer cells. Cancer Res, 61, 1862-1868.
- Calka J, McDonald JK, Ojeda SR (1988): The innervation of the immature rat ovary by calcitonin gene- related peptide. Biol Reprod, 39, 1215– 1223.
- Delfino F, Walker WH (1998): Stage-specific nuclear expression of NF-kB in mammalian testis. Mol Endocrinol, 12, 1696–1707.
- Deveraux QL, Reed JC (1999): IAP family proteins \320 suppressors of apoptosis. Genes Dev, 13, 239-252.
- Deveraux QL, Roy N, Steinicke HR, Van A, Zhou Q, Srinivasula SM, Alnemri ES, Salvesen GS, Reed JC (1998): IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J, 17, 2215–2223.
- Fitzgerald M (1983): Capsaicin and sensory neurons: a review. Pain, 15, 109-130.
- Geymayer S, Doppler W (2000): Activation of NF-_B p50/p65 is regulated in the developing mammary gland and inhibits STAT5-mediated-casein gene expression. FASEB, 14, 1159–1170.
- Jensen- Jaorlim E, Gajdzik L, Haberl I (1998): Hot spices influence permeability of human intestinal epithelial monolayers. J Nutr, 128, 577-581.
- Kannisto P, Ekblad E, Helm G, Owman C, Sjöberg NO, Stjernquist M, Sundler F, Walles B (1986): Existence and coexistence of peptides in nerves of the mammalian ovary and oviduct demonstrated by immunocytochemistry. Histochemistry, 86, 25–34.
- Klein CM, Burden HW (1988): Substance P and vasoactive intestinal polypeptide (VIP) immunoreactive nerve fibers in relation to ovarian postganglionic perikarya in para and prevertebral ganglia: evidence from combined retrograde tracing and immunocytochemistry. Cell Tissue Res, 252, 403–410.
- Moran C, Morales L, Selene Razo R, Apolonio J, Quiroz U, Chavira R, Domınguez R (2003): Effects of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy. Life Sci, 73, 2113–2125.
- Nance DM, King TR, Nance PW (1987): 21. Wang Y, Asselin E, Tsang BK (2002): Involvement of Neuroendocrine and behavioral effects of intrathecal capsaicin in adult female rats. Brain Res Bull, 18, 109–114.
- Oh TW, Ohta F (2003): Dose-dependent effect of capsaicin on endurance capacity in rats. Br J Nutr, 90, 515-520.
- Panossian A, Gabrielian E, Wagner H (1996): Dose- dependent reversible effects of capsaicin on interleukin- 1 a production is associated with the metabolism of arachidonic acid (leukotriene B4 and prostaglandin E2) as well as nitric oxide production in human leucocytes. Phytomedicine, 3, 169-174.
- Papka RE, Cotton JP, Traurig HH (1985): Comparative distribution of neuropeptide tyrosine-, vasoactive intestinal polypeptide-, substance P-inmunoreactive, acetylcholinesterase- positive and noradrenergic nerves in the reproductive tract of the female rat. Cell Tissue Res, 242, 475–490.
- Pedersen T, Peters H (1968): Proposal for a classification of oocytes and follicles in the mouse ovary. J Reprod Fertil, 17, 555-557.
- Toth B, Gannett P (1992): Carcinogenicity of lifelong administration of capsaicin of hot pepper in mice. In Vivo, 6, 59-63.
- True LD (1990): Principles of immunohistochemistry. 16- 22, In: True LD, (ed), Atlas of Diagnostic Immunohistopathologhy. Gower Medical Publishing, New York.
- Vaishnava P, Wang DH (2003): Capsaicin sensitive- sensory nerves and blood pressure regulation. Curr Med Chem Cardiovasc Hematol Agents, 1, 177-88.
- Vaskivuo TE, Ottander U, Oduwole O, Isomaa V,
- Vihko P, Olofsson JI, Tapanainen JS (2002): Role of
- apoptosis, apoptosis-related factors and 17β
- hydroxysteroid dehydrogenases in human corpus luteum
- regression. Mol Cell Endocrinol, 194, 191-200. TGFα in the regulation of rat ovarian X-linked inhibitor of apoptosis protein expression and follicular growth by FSH. Biol Reprod, 66, 1672-1680.
- Wang Y, Chan S, Tsang BK (2002): Involvement of inhibitory nuclear factor-kB (NF-kB)-independent NFkB activation in the gonadotropic regulation of X-linked inhibitor of apoptosis expression during ovarian follicular development in vitro. Endocrinology, 143, 2732–2740.
- Wang Y, Rippstein PU, Tsang BK (2003): Role and gonadotrophic regulation of X-linked inhibitor of apoptosis protein expression during rat ovarian follicular development in vitro. Biol Reprod, 68, 610–619.
- Xiao CW, Ash K, Tsang BK (2001): Nuclear factor-kB- mediated X-linked inhibitor of apoptosis protein expression prevents rat granulosa cells from tumor necrosis factor induced apoptosis. Endocrinology, 142, 557–563.
- Yu SM, Lin KH (2002): Morphological alterations in the trachea of capsaicin-pretreated rat during postnatal development. Zool. Stud, 41, 13-22.
- Zık B, Özgüden C, Tütüncü Ş, İlhan T (2007): Expression of vanilloid receptor-1 in the duodenum of the capsaicin treated rat. Bull Vet Inst Pulawy, 51, 149-153.
Abstract
Capsaicin (CAP), kırmızı acı biberin acı bileşimidir. Yüksek doz CAP’ın ovaryum üzerine etkisi bilinmesine rağmen, düşük doz CAP’ın etkisi bilinmemektedir. Sunulan çalışmanın amacı, günlük diyetle kırmızı acı biberden alacağımız miktardaki CAP’ın, ovaryum follikül gelişiminde etkili olan X-linked Inhibitor of Apoptosis Protein (XIAP) ve Nuclear Factor-kappaB (NF-kB) proteinlerinin sentezi üzerine etkisini ortaya koymaktır. Çalışmada 21 günlük 80 adet Sprague-Dawley ırkı dişi sıçanlar kullanıldı. Hayvanlar enjeksiyon periyoduna göre 4 gruba ayrıldı (6, 9, 12 ve 15 gün). Deney sonrasında ovaryum kesitlerine immunohistokimyasal olarak XIAP ve NF-kB antikorları kullanılarak indirekt Streptavidin- Biotin- Peroksidaz yöntemi uygulandı. Ovaryum dokusunda XIAP ve NF-kB immunreaksiyonu, ovaryum yüzeyindeki sağlıklı tüm follikül sınıflarında gözlenirken, atretik folliküllerin granuloza ve teka hücrelerinde boyanma gözlenmedi. Bununla birlikte oosit, ovaryum yüzey epitel hücreleri ve intersitisyel hücrelerde de sentez belirlendi.XIAP ve NF-kB protein sentezlerinin birbirine bağımlı olarak geliştiği ve özellikle 15 gün CAP uygulanan grupta kontrol A ve kontrol B grubuna göre bu proteinlerin sentezinin yüksek olduğu gözlendi. Sonuç olarak kısa süreli düşük doz CAP uygulamasının NF-kB yoluyla XIAP sentezini uyardığını ve follikülleri atreziden koruduğunu, dolayısıyla düşük doz CAP’ın ovaryum follikül gelişiminin düzenlenmesine yardımcı olan faktörlerden biri olabileceğini düşünmekteyiz
Keywords
References
- Asselin E, Mills GB, Tsang BK (2001): XIAP regulates Akt activity and caspase-3-dependent cleavage during cisplatin-induced apoptosis in human ovarian epithelial cancer cells. Cancer Res, 61, 1862-1868.
- Calka J, McDonald JK, Ojeda SR (1988): The innervation of the immature rat ovary by calcitonin gene- related peptide. Biol Reprod, 39, 1215– 1223.
- Delfino F, Walker WH (1998): Stage-specific nuclear expression of NF-kB in mammalian testis. Mol Endocrinol, 12, 1696–1707.
- Deveraux QL, Reed JC (1999): IAP family proteins \320 suppressors of apoptosis. Genes Dev, 13, 239-252.
- Deveraux QL, Roy N, Steinicke HR, Van A, Zhou Q, Srinivasula SM, Alnemri ES, Salvesen GS, Reed JC (1998): IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J, 17, 2215–2223.
- Fitzgerald M (1983): Capsaicin and sensory neurons: a review. Pain, 15, 109-130.
- Geymayer S, Doppler W (2000): Activation of NF-_B p50/p65 is regulated in the developing mammary gland and inhibits STAT5-mediated-casein gene expression. FASEB, 14, 1159–1170.
- Jensen- Jaorlim E, Gajdzik L, Haberl I (1998): Hot spices influence permeability of human intestinal epithelial monolayers. J Nutr, 128, 577-581.
- Kannisto P, Ekblad E, Helm G, Owman C, Sjöberg NO, Stjernquist M, Sundler F, Walles B (1986): Existence and coexistence of peptides in nerves of the mammalian ovary and oviduct demonstrated by immunocytochemistry. Histochemistry, 86, 25–34.
- Klein CM, Burden HW (1988): Substance P and vasoactive intestinal polypeptide (VIP) immunoreactive nerve fibers in relation to ovarian postganglionic perikarya in para and prevertebral ganglia: evidence from combined retrograde tracing and immunocytochemistry. Cell Tissue Res, 252, 403–410.
- Moran C, Morales L, Selene Razo R, Apolonio J, Quiroz U, Chavira R, Domınguez R (2003): Effects of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy. Life Sci, 73, 2113–2125.
- Nance DM, King TR, Nance PW (1987): 21. Wang Y, Asselin E, Tsang BK (2002): Involvement of Neuroendocrine and behavioral effects of intrathecal capsaicin in adult female rats. Brain Res Bull, 18, 109–114.
- Oh TW, Ohta F (2003): Dose-dependent effect of capsaicin on endurance capacity in rats. Br J Nutr, 90, 515-520.
- Panossian A, Gabrielian E, Wagner H (1996): Dose- dependent reversible effects of capsaicin on interleukin- 1 a production is associated with the metabolism of arachidonic acid (leukotriene B4 and prostaglandin E2) as well as nitric oxide production in human leucocytes. Phytomedicine, 3, 169-174.
- Papka RE, Cotton JP, Traurig HH (1985): Comparative distribution of neuropeptide tyrosine-, vasoactive intestinal polypeptide-, substance P-inmunoreactive, acetylcholinesterase- positive and noradrenergic nerves in the reproductive tract of the female rat. Cell Tissue Res, 242, 475–490.
- Pedersen T, Peters H (1968): Proposal for a classification of oocytes and follicles in the mouse ovary. J Reprod Fertil, 17, 555-557.
- Toth B, Gannett P (1992): Carcinogenicity of lifelong administration of capsaicin of hot pepper in mice. In Vivo, 6, 59-63.
- True LD (1990): Principles of immunohistochemistry. 16- 22, In: True LD, (ed), Atlas of Diagnostic Immunohistopathologhy. Gower Medical Publishing, New York.
- Vaishnava P, Wang DH (2003): Capsaicin sensitive- sensory nerves and blood pressure regulation. Curr Med Chem Cardiovasc Hematol Agents, 1, 177-88.
- Vaskivuo TE, Ottander U, Oduwole O, Isomaa V,
- Vihko P, Olofsson JI, Tapanainen JS (2002): Role of
- apoptosis, apoptosis-related factors and 17β
- hydroxysteroid dehydrogenases in human corpus luteum
- regression. Mol Cell Endocrinol, 194, 191-200. TGFα in the regulation of rat ovarian X-linked inhibitor of apoptosis protein expression and follicular growth by FSH. Biol Reprod, 66, 1672-1680.
- Wang Y, Chan S, Tsang BK (2002): Involvement of inhibitory nuclear factor-kB (NF-kB)-independent NFkB activation in the gonadotropic regulation of X-linked inhibitor of apoptosis expression during ovarian follicular development in vitro. Endocrinology, 143, 2732–2740.
- Wang Y, Rippstein PU, Tsang BK (2003): Role and gonadotrophic regulation of X-linked inhibitor of apoptosis protein expression during rat ovarian follicular development in vitro. Biol Reprod, 68, 610–619.
- Xiao CW, Ash K, Tsang BK (2001): Nuclear factor-kB- mediated X-linked inhibitor of apoptosis protein expression prevents rat granulosa cells from tumor necrosis factor induced apoptosis. Endocrinology, 142, 557–563.
- Yu SM, Lin KH (2002): Morphological alterations in the trachea of capsaicin-pretreated rat during postnatal development. Zool. Stud, 41, 13-22.
- Zık B, Özgüden C, Tütüncü Ş, İlhan T (2007): Expression of vanilloid receptor-1 in the duodenum of the capsaicin treated rat. Bull Vet Inst Pulawy, 51, 149-153.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA36AB69BE |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 4 |