Abstract
The study was projected to determine immunohistochemically vimentin positive cells by using anti-vimentin primary antibody, to eludicate ultrastructurally the morphological properties of these cells and, to examined the presence of villous M cells in villi epithelia. In this study, ten healthy, adult Angora rabbits were used as a material. It was examined the ileum with and without Peyer’s patches. Immunohistochemical examination revealed the mature and immature M cells within FAE’s and cup cells within epithelium of villi exibited the positive vimentin immunoreactivity. Electron microscopical examination revealed the mature M cells surrounded the intraepithelial lymphocytes (IEL) by a basolateral invagination. Whereas, immature M cells and cup cells were observed to be associated with one or two intraepithelial lymphocytes without such a pocketing. No villous M cells were found within epithelia of ileal villi. In conclusion, the cup cells are similar to immature M cells associated with IEL without a intraepithelial pocketing and showed a vimentin positive immunoreaction within their cytoplasms, but these cells are not villous M cells or enterocytes
Keywords
References
- Beyaz F, Aştı RN (2004): Development of ileal Peyer’s patches and follicle associated epithelium in bovine foetuses. Anat Histol Embryol, 33, 172-179.
- Cesta MF (2006): Normal structure, function, and histology of mucosa-associated lymphoid tissue. Toxicol Pathol, 34, 599-608.
- Corr SC, Gahan CGM, Hill C (2008): M-cells: Origin, morphology and role in mucosal immunity and microbial pathogenesis. FEMS Immunol Med Microbiol, 52, 2-12.
- Fujimura Y, Iida M (2001): A new marker for cup cells in the rabbit small intestine: expression of vimentin intermediate filament protein. Med Electron Microsc, 34, 223-229.
- Gebert A, Hach G, Bartels H (1992): Co-localization of vimentin and cytokeratins in M-cells of rabbit gut- associated lymphoid tissue (GALT). Cell Tissue Res, 269, 331-340.
- Gebert A, Fassbender S, Werner K, Weissferdt A (1999): The development of M cells in Peyer’s patches is restricted to specialized dome-associated crypts. Am J Pathol, 154, 1573-1582.
- Goldman RD, Khuon S, Chou YH, Opal P, Steinert PM (1996): The function of intermediate filaments in cell shape and cytoskeletal integrity. J Cell Biol, 134, 971-983.
- Hathaway LJ, Kraehenbuhl JP (2000): The role of M cells in mucosal immunity. Cell Mol Life Sci, 57, 323-332.
- Helfand BT, Chou YH, Shumaker DK, Goldman RD (2005): Intermediate filament proteins participate in signal transduction. Trends Cell Biol, 15, 568-570.
- Ivaska J, Pallari HM, Nevo J, Eriksson JE (2007): Novel functions of vimentin in cell adhesion, migration, and signaling. Experimental Cell Res, 313, 2050-2062.
- Iwatsuki H, Ogawa C, Suda M (2002): Vimentin-positive cells in the villus epithelium of the rabbit small intestine. Histochem Cell Biol, 117, 363-370.
- Jang MH, Kweon MN, Iwatani K, Yamamoto M, Terahara K, Sasakawa C, Suzuki T, Nochi T, Yokota Y (2004): Intestinal villous M cells: An antigen entry site in the mucosal epithelium. PNAS, 101, 6110-6115.
- Jepson MA, Mason CM, Bennett MK, Simmons NL, Hirst BH (1992): Co-expression of vimentin and cytokeratins in M-cells of rabbit intestinal lymphoid follicle associated eptihelium. Histochem J, 24, 33-39.
- Jepson MA, Simmons NL, Hirst GL, Hirst BH (1993): Identification of M cells and their distribution in rabbit intestinal Peyer patches and appendix. Cell Tissue Res, 273, 127-136.
- Kerneis S, Bogdanova A, Kraehenbuhl JP, Pringault E (1997): Conversion by Peyer’s patch lymphocytes of human enterocytes into M cells that transport bacteria. Science, 277, 949-952.
- Lelouard H, Sahuquet A, Reggio H, Montcourrier P (2001): Rabbit M cells and dome enterocytes are distinct cell lineages. J Cell Sci, 114, 2077-2083.
- Mach J, Hshieh T, Hsieh D, Grubbs N, Chervonsky A (2005): Development of intestinal M cells. Immunol Rev, 206, 177-189.
- Magalhaes JG, Tattoli I, Girardin SE (2007): The intestinal epithelial barrier: How to distinguish between the microbial flora and pathogens. Semin Immunol, 19, 106-115.
- Madara JL (1982): Cup cells: structure and distribution of a unique class of epithelial cells in guinea pig, rabbit, and monkey small intestine. Gastroenterology, 83, 981- 994.
- Madara JL, Carlson SL (1985): Cup cells: further structural characterization of the brush border and the suggestion that they may serve as an attachment site for an unidentified bacillus in guinea pig ileum. Gastroenterology, 89, 1374-1386.
- Marshmann E, Booth C, Potten CS (2002): The intestinal epithelial stem cell. BioEssas, 24, 91-98.
- Miller H, Zhang J, Koulee R, Patel GB, Chen W (2007): Intestinal M cells: The fallible sentinels? World J Gastroenterol, 13, 1477-1486.
- Neutra MR, Mantis NJ, Kraehenbuhl JP (2001): Collaboration of epithelial cells with organized mucosal lymphoid tissues. Immunol Nature, 2, 1004-1009.
- Newberry RD (2008): Intestinal lymphoid tissues: is variety an asset or a liability. Curr Opin Gastroenterol, 24, 121-128.
- Onishi S, Yokoyama T, Chin K, Yuji M, Inamoto T, Qi WM, Warita K, Hoshi N, Kitagawa H (2007): Ultrastructural study of the differentiation and the fate of M cells in follicle-asssociated epithelium of rat Peyer’s patch. J Vet Med Sci, 69, 501-508.
- Potten CS, Booth C, Pritchard DM (1997): The intestinal epithelial stem cell: the mocosal governor. Int J Exp Path, 78, 219-243.
- Ramirez C, Gebert A (2003): Vimentin-positive cells in the epithelium of rabbit ileal villi represent cup cells but not M-cells. J Histochem Cytochem, 51, 1533-1544.
Abstract
Çalışma, Ankara tavşanı ileum epitelinde vimentin pozitif hücreleri anti-vimentin primer antikoru yardımıyla immunohistokimyasal olarak belirlemek, ince yapı düzeyinde bu hücrelerin sahip oldukları morfolojik özellikleri ortaya koymak ve villus epitellerinde FAE dışı villöz M hücrelerinin varlığını araştırmak amacıyla planlanmıştır. Çalışmada materyal olarak, on adet sağlıklı, erişkin Ankara tavşanı kullanıldı. İleumun Peyer plakları içeren ve içermeyen bölgeleri incelendi. İleal Peyer plaklarında dom bölgelerinin yüzeylerini örten folikülle ilişkili epitellerdeki (FAE) olgun ve olgunlaşmamış M hücreleri ile villus intestinalis epitellerindeki kupa hücreleri vimentin pozitif immunreaksiyon gösterdi. Elektron mikroskobik incelemede, olgun M hücrelerinin bazolateral bir invaginasyonla intraepiteliyal lenfositleri (İEL) çevrelediği görüldü. Olgunlaşmamış M hücreleri ve kupa hücrelerinin ise böyle bir paketlenme olmaksızın bir veya iki adet İEL’le yakın ilişki içerisinde olduğu belirlendi. Villöz M hücreleri ise ileal villus epitellerinde tespit edilemedi. Sonuç olarak, kupa hücrelerinin bir intraepiteliyal paketlenme olmaksızın İEL’lerle yakın ilişki içerisinde olması ve sitoplazmasında vimentin pozitif immunreaktivite göstermeleri nedeniyle olgunlaşmamış M hücrelerine benzedikleri, ancak ince yapı özellikleri değerlendirildiğinde bu hücrelerin villöz M hücreleri veya enterosit olmadıkları anlaşıldı
Keywords
References
- Beyaz F, Aştı RN (2004): Development of ileal Peyer’s patches and follicle associated epithelium in bovine foetuses. Anat Histol Embryol, 33, 172-179.
- Cesta MF (2006): Normal structure, function, and histology of mucosa-associated lymphoid tissue. Toxicol Pathol, 34, 599-608.
- Corr SC, Gahan CGM, Hill C (2008): M-cells: Origin, morphology and role in mucosal immunity and microbial pathogenesis. FEMS Immunol Med Microbiol, 52, 2-12.
- Fujimura Y, Iida M (2001): A new marker for cup cells in the rabbit small intestine: expression of vimentin intermediate filament protein. Med Electron Microsc, 34, 223-229.
- Gebert A, Hach G, Bartels H (1992): Co-localization of vimentin and cytokeratins in M-cells of rabbit gut- associated lymphoid tissue (GALT). Cell Tissue Res, 269, 331-340.
- Gebert A, Fassbender S, Werner K, Weissferdt A (1999): The development of M cells in Peyer’s patches is restricted to specialized dome-associated crypts. Am J Pathol, 154, 1573-1582.
- Goldman RD, Khuon S, Chou YH, Opal P, Steinert PM (1996): The function of intermediate filaments in cell shape and cytoskeletal integrity. J Cell Biol, 134, 971-983.
- Hathaway LJ, Kraehenbuhl JP (2000): The role of M cells in mucosal immunity. Cell Mol Life Sci, 57, 323-332.
- Helfand BT, Chou YH, Shumaker DK, Goldman RD (2005): Intermediate filament proteins participate in signal transduction. Trends Cell Biol, 15, 568-570.
- Ivaska J, Pallari HM, Nevo J, Eriksson JE (2007): Novel functions of vimentin in cell adhesion, migration, and signaling. Experimental Cell Res, 313, 2050-2062.
- Iwatsuki H, Ogawa C, Suda M (2002): Vimentin-positive cells in the villus epithelium of the rabbit small intestine. Histochem Cell Biol, 117, 363-370.
- Jang MH, Kweon MN, Iwatani K, Yamamoto M, Terahara K, Sasakawa C, Suzuki T, Nochi T, Yokota Y (2004): Intestinal villous M cells: An antigen entry site in the mucosal epithelium. PNAS, 101, 6110-6115.
- Jepson MA, Mason CM, Bennett MK, Simmons NL, Hirst BH (1992): Co-expression of vimentin and cytokeratins in M-cells of rabbit intestinal lymphoid follicle associated eptihelium. Histochem J, 24, 33-39.
- Jepson MA, Simmons NL, Hirst GL, Hirst BH (1993): Identification of M cells and their distribution in rabbit intestinal Peyer patches and appendix. Cell Tissue Res, 273, 127-136.
- Kerneis S, Bogdanova A, Kraehenbuhl JP, Pringault E (1997): Conversion by Peyer’s patch lymphocytes of human enterocytes into M cells that transport bacteria. Science, 277, 949-952.
- Lelouard H, Sahuquet A, Reggio H, Montcourrier P (2001): Rabbit M cells and dome enterocytes are distinct cell lineages. J Cell Sci, 114, 2077-2083.
- Mach J, Hshieh T, Hsieh D, Grubbs N, Chervonsky A (2005): Development of intestinal M cells. Immunol Rev, 206, 177-189.
- Magalhaes JG, Tattoli I, Girardin SE (2007): The intestinal epithelial barrier: How to distinguish between the microbial flora and pathogens. Semin Immunol, 19, 106-115.
- Madara JL (1982): Cup cells: structure and distribution of a unique class of epithelial cells in guinea pig, rabbit, and monkey small intestine. Gastroenterology, 83, 981- 994.
- Madara JL, Carlson SL (1985): Cup cells: further structural characterization of the brush border and the suggestion that they may serve as an attachment site for an unidentified bacillus in guinea pig ileum. Gastroenterology, 89, 1374-1386.
- Marshmann E, Booth C, Potten CS (2002): The intestinal epithelial stem cell. BioEssas, 24, 91-98.
- Miller H, Zhang J, Koulee R, Patel GB, Chen W (2007): Intestinal M cells: The fallible sentinels? World J Gastroenterol, 13, 1477-1486.
- Neutra MR, Mantis NJ, Kraehenbuhl JP (2001): Collaboration of epithelial cells with organized mucosal lymphoid tissues. Immunol Nature, 2, 1004-1009.
- Newberry RD (2008): Intestinal lymphoid tissues: is variety an asset or a liability. Curr Opin Gastroenterol, 24, 121-128.
- Onishi S, Yokoyama T, Chin K, Yuji M, Inamoto T, Qi WM, Warita K, Hoshi N, Kitagawa H (2007): Ultrastructural study of the differentiation and the fate of M cells in follicle-asssociated epithelium of rat Peyer’s patch. J Vet Med Sci, 69, 501-508.
- Potten CS, Booth C, Pritchard DM (1997): The intestinal epithelial stem cell: the mocosal governor. Int J Exp Path, 78, 219-243.
- Ramirez C, Gebert A (2003): Vimentin-positive cells in the epithelium of rabbit ileal villi represent cup cells but not M-cells. J Histochem Cytochem, 51, 1533-1544.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA34JR93BJ |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 4 |