Abstract
Tumor suspected operation materials obtained from eight parakeets are evaluated clinically, pathologically and immunohistochemically. The age of the parakeets varied from seven months to nine years old. Four were male, one was a female and the sexes of the rest three were not identified. Six of the operation materials were taken from the above the tail while the other two from beneath the wings. Evaluated tumors were observed to be hard in consistency and whitish color on cut surface. At histopathological examination; it is seen that hyperchromatic nucleated, big, ovoid shaped, pale eosinophilic cytoplasmated cells had formed groups in dermis. Keratinized horn pearl formations are identified in the centers of most of these cell groups. Within the horn pearl formations hyperkeratotic, parakeratotic changes and calcifications are found. In some cases inflammation areas consisting of heterophil leukocytes, lymphocytes and plasma cells in the dermis are observed. Also in histopathological examination of one case, tubercle formations made of coagulation necrosis in the center surrounded by heterophil leukocytes, macrophages, epithelioid cells, lymphocytes and foreign body giant cells are found as well as tumoral formations. Groups of cudgel shaped, red colored bacteria are found in Ziehl-Neelsen staining of the sections. Tissues are stained for proliferating cell nuclear antigen (PCNA) and p53 proteins immunohistochemically in order to interpret mitotic activity and malignancy of the tumor. TUNEL method is applied to the tissues to evaluate apoptosis
Keywords
Dermal squamous cell carcinoma histopathology immunohistochemistry keratoacanthoma parakeet
References
- Chu LL, Rutteman GR, Kong JMC, Ghahremani M, Schmeing M, Misdorp W, Garderen E, Pelletier J (1998): Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia. Breast Cancer Res Treat, 50,11-25.
- Cooper BJ (2002): Disease at the cellular level. 16-75. In DO Slauson, BJ Cooper (eds), Mechanisms of Disease. Mosby, Missouri.
- Davis PL, Iglehart JD (1998): p53 Detection in Breast Cancer. 319-330. In M Hanausek, Z Walaszek (eds), Tumor Marker Protocols. Humana Press: Totowa, New Jersey.
- Fallavena LCB, Canal CW, Salle CTP, Morares HLS, Rocha SLS, Pereira RA, Silva AB (2002): Presence of avipoxvirus DNA in avian dermal squamous cell carcinoma. Avian Pathol, 31, 241-246.
- Hafner S, Goodwin MA (1997): Dermal squamous cell carcinoma. 1044-1046. In BW Calnek, HJ Barnes, CW Beard, LR McDougal, YM Saif (eds) Diseases of Poultry 10th edn. Ames, Iowa State Press.
- Hafner S, Harmon BG, Rowland GN, Steward RG, Glisson JR (1991): Spontaneous Regression of “Dermal Squamous Cell Carcinoma” in Young Chickens. Avian Dis, 35, 321-327.
- Hafner S, Harmon BG, Steward RG, Rowland GN (1993): Avian Keratoacanthoma (Dermal Squamous Cell Carcinoma) in Broiler Chicken Carcasses. Vet Pathol, 30, 265-270.
- Reece RL (1997): Tumors of Unkown Etiology. 489-510. In BW Calnek, HJ Barnes, CW Beard, LR McDougal, YM Saif Diseases of Poultry 10th edn. Ames, Iowa State Press.
- Taylor CL (1994): Principal antibodies applicable to paraffin sections. 83-84. In CR Taylor, RJ Cote. Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist 2 nd edn. WB Saunders Company: Pennsylvania.
- Turnquest RU (1979): Dermal Squamous Cell Carcinoma in Young Chickens. American Journal of Vet Res, 40, 1628-1633.
- Weinstock D, Correa MT, Rives DV, Wages DP (1995): Histopathology and Epidemiology of Condemnations due to Squamous Cell Carcinoma in Broiler Chickens in North Carolina. Avian Dis, 39, 676-686.
Abstract
Tümör şüpheli sekiz muhabbet kuşundan alınmış operasyon materyalleri klinik, patolojik ve immunhistokimyasal olarak değerlendirildi. İncelenen muhabbet kuşları 7ay- 9 yaş arasındaydı ve dördü erkek, biri dişi iken üç tanesinin cinsiyeti bildirilmemişti. Operasyon materyallerinin altısı kuyruk üzerinden ikisi ise kanat altından alınmıştı. İncelenen tümörlerin sert kıvamlı ve kesit yüzlerinin ise beyazımtırak renkte olduğu gözlendi. Tümöral kitlelerin histopatolojik incelenmesinde; dermiste hiperkromatik çekirdekli, büyük, ovoid şekilli, solgun eozinofilik sitoplazmalı hücrelerin gruplar oluşturduğu dikkati çekti. Bu hücre gruplarının bir çoğunun orta kısımlarında keratinize glob korne yapıları belirlendi. Glob korne yapıları içerisinde hiperkeratotik ve parakeratotik değişiklikler ile kalsifikasyonlara rastlandı. Olguların bazılarında dermiste nötrofil lökosit, lenfosit ve plazma hücrelerinden oluşan yangısal alanlar görüldü. Ayrıca bir olguda, saptanan tümörden başka aynı hayvandan alınan kitlelerin histopatolojik incelenmesinde ise; ortada koagulasyon nekrozu alanının etrafında heterofil lökositler, histiyositler, epiteloid hücreler, lenfositler ve yabancı cisim dev hücrelerinin oluşturduğu tüberkül yapısına rastlandı. Yapılan Ziehl-Neelsen boyamasında kırmızı renkli, çomak şekilli bakteri kümeleri görüldü. Mitotik aktiviteleri ve malignitelerini yorumlamak üzere dokular PCNA ve p53 proteinleri ile immunhistokimyasal olarak boyandı. Apoptozisin değerlendirilmesi için TUNEL metodu uygulandı
References
- Chu LL, Rutteman GR, Kong JMC, Ghahremani M, Schmeing M, Misdorp W, Garderen E, Pelletier J (1998): Genomic organization of the canine p53 gene and its mutational status in canine mammary neoplasia. Breast Cancer Res Treat, 50,11-25.
- Cooper BJ (2002): Disease at the cellular level. 16-75. In DO Slauson, BJ Cooper (eds), Mechanisms of Disease. Mosby, Missouri.
- Davis PL, Iglehart JD (1998): p53 Detection in Breast Cancer. 319-330. In M Hanausek, Z Walaszek (eds), Tumor Marker Protocols. Humana Press: Totowa, New Jersey.
- Fallavena LCB, Canal CW, Salle CTP, Morares HLS, Rocha SLS, Pereira RA, Silva AB (2002): Presence of avipoxvirus DNA in avian dermal squamous cell carcinoma. Avian Pathol, 31, 241-246.
- Hafner S, Goodwin MA (1997): Dermal squamous cell carcinoma. 1044-1046. In BW Calnek, HJ Barnes, CW Beard, LR McDougal, YM Saif (eds) Diseases of Poultry 10th edn. Ames, Iowa State Press.
- Hafner S, Harmon BG, Rowland GN, Steward RG, Glisson JR (1991): Spontaneous Regression of “Dermal Squamous Cell Carcinoma” in Young Chickens. Avian Dis, 35, 321-327.
- Hafner S, Harmon BG, Steward RG, Rowland GN (1993): Avian Keratoacanthoma (Dermal Squamous Cell Carcinoma) in Broiler Chicken Carcasses. Vet Pathol, 30, 265-270.
- Reece RL (1997): Tumors of Unkown Etiology. 489-510. In BW Calnek, HJ Barnes, CW Beard, LR McDougal, YM Saif Diseases of Poultry 10th edn. Ames, Iowa State Press.
- Taylor CL (1994): Principal antibodies applicable to paraffin sections. 83-84. In CR Taylor, RJ Cote. Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist 2 nd edn. WB Saunders Company: Pennsylvania.
- Turnquest RU (1979): Dermal Squamous Cell Carcinoma in Young Chickens. American Journal of Vet Res, 40, 1628-1633.
- Weinstock D, Correa MT, Rives DV, Wages DP (1995): Histopathology and Epidemiology of Condemnations due to Squamous Cell Carcinoma in Broiler Chickens in North Carolina. Avian Dis, 39, 676-686.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA59DS46BD |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 4 |