Abstract
The endocrine pancreas of the Angora rabbits (10 healthy adult) was examined in this study using histochemistry and electron microscopy. Pancreas samples taken rabbits were fixed in Bouin, Carnoy, and gluteraldehyde-paraformaldehyde solutions. Although, two endocrine cell types was determined in sections of pancreas staining as histochemical by aldehyde fuchsine light green orange G (AF), endocrine islets were constituted by A, B and D cells in electron microscopical examination. The centre of the Langerhans islets were occupied predominantly by B cells stained purple-violet and surrounded a few by A cells stained yellowish-green. Secretory granules of A cells generally were spherical and high electron-dense whereas in the cytoplasm of B cells were determined high electron-dense and low electron- dens granules, have a halo between the membrane and their content. D cells were observed contain to round two kinds of secretory granules, have high electron-dense and low electron-dense material, but smaller than those A and B cell types. In conclusion, the endocrine pancreas of the Angora rabbits were constituted by numerously B cells, a few A cells, and infrequently D cells
References
- Adeghate E, Ponery AS (2002): Ghrelin stimulates insulin secretion from the pancreas of normal and diabetic rats. J Neuroendocrinol, 14, 555– 560.
- Ali SS, Ali MM, Hering BJ, Bretzel RG, Federlin K (1991): Light- and electron-microscopic studies on isolated bovine islets of Langerhans. Anat Anz, 173, 73– 80.
- Aluments J, Sundler F, Hakånson R (1977): Distribution, ontogeny and ultrastructure of somatostatin immunreactive cells in the pancreas and gut. Cell Tissue Res, 185, 465– 479.
- Bacha WJ, Wood LM (1990): Color Atlas of Veterinary Histology. Lea and Febiger, Philadelphia & London
- Bancroft JD, Cook HC (1984): Manual of Histological Techniques. Churchill Livingstone Medical Division Longman Group Limited, UK.
- Baskin DG, Gorray KC, Fujimoto WY (1984): Immunocytochemical identification of cells containing insulin, glucagon, somatostatin, and pancreatic polypeptide in the islets of Langerhans of the guinea pig pancreas with light and electron microscopy. Anat Rec, 208, 567– 578.
- Bencosme SA, Pease DC (1958): Electron microscopy of the pancreatic islets. Endocrinology, 63, 1– 13.
- Bertelli E, Regoli M, Bastianini A (1994): Endocrine tissue associated with the pancreatic ductal system: a light and electron microscopic study of the adult rat pancreas with special reference to a new endocrine arrangement. Anat Rec, 239, 71– 78.
- Bloom W, Fawcett DW (1994): A Textbook of Histology. Twelfth Ed. Chapman and Hall, Sounders Company, Philadelphia, London, Toronto.
- Böck P, Moneim M, Egerbacher M (1997): Development of pancreas. Microsc Res Techn, 37, 374– 383.
- Cowap J (1985): The first appearence of endocrine cells in the splenic lobe of the embriyonic chick pancreas. Gen Comp Endocrin, 60, 131– 137.
- Culling CFA, Allison RT, Barr WT (1985): Cellular Pathology Technique. Fourth Ed. Butterworth & Co. Ltd. London.
- Dellmann HD, Eurell J (1998): Textbook of Veterinary Histology. 5nd. Ed. Williams & Wilkins A Waverly Company, Pennsylvania, USA
- El-Naggar MM (2000): Ultrastructural immunogold study on the various cell types of cultured pancreatic islets of adult rats. Folia Morphol, 59, 253– 262.
- Greider MH, Gersell DJ, Gingerich RL (1978): Ultrastructural localization of pancreatic polypeptide in the F cell of the dog pancreas. J Histochem Cytochem, 26, 1103– 1108.
- Gomez–Acebo J, Parrilla R, R-Candela JL (1968): Fine structure of the A and D cells of the rabit endocrine pancreas in vivo and incubated in vitro. I. mechanism of secretion of the a cells. J Cell Biol, 36, 33– 44.
- Helmstaedter V, Feurle GE, Forssmann WG (1976): Insulin-, glucagon-, and somatostatin-immunoreactive endocrine cells in the equine pancreas. Cell Tissue Res, 172, 447– 454.
- Kaung CH (1985): Electron microscopic immunocytochemical localization of glucagon and pancreatic polipeptide in rat pancreas: characterization of a population of islet cells containing both peptides. Anat Rec, 212, 292– 300.
- Karnovsky MJ (1965): Formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol, 27, 137A– 138A.
- Lacy PE (1957): Electron microscopic identification of different cell types in the islets of Langerhans of the guinea pig, rat, rabbit and dog. Anat Rec, 128, 255– 267.
- Larsson LI (1998): On the development of the islets of Langerhans. Microsc Res Tech, 43, 284– 291.
- Liman N (1991): Sığır, Koyun ve Keçi’nin Pankreasında A ve B Hücreleri Üzerine Morfolojik çalışmalar. Doktora Tezi, Ankara Üniv. Sağ. Bil. Ens. Ankara.
- Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L (2004): Ghrelin cells replace insulin- producing B cells in two mouse models of pancreas development. Proc Natl Acad Sci, 101, 4679– 4684.
- Reddy S, Elliott RB (1985): Insulin, glucagon, pancreatic polypeptide hormone and somatostatin in the goat pancreas: demonstration by immunocytochemistry. Ausr J Biol Sci, 38, 59– 66.
- Slack JMW (1995): Developmental biology of the pancreas. Development, 121, 1569–1580.
- Şimşek N, Özudoğru Z, Alabay B (2008): Immunohistochemical studies on the splenic lobe of the pancreas in young japanese quails (Coturnix c. japonica), DTW, 115, 189– 193.
- Veneable JH, Coggeshall R (1965): A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25, 407– 408.
- Wolfe-Coote SA, Louw J, Woodroof CW, Heydenrych JJ, Du Toit DF (1998): Induction of cell proliferation an differentiation in the pancreas of the adult Vervet Monkey (Cercopithecus Aethiops). Pancreas, 16, 129– 133.
- Young B, Heath JW (2000): Wheater’s Functional Histology. Fourth Ed. Churchill Livingstone, Sydney, Toronto.
- Yukawa M, Takeuchi T, Watanabe T, Kitamura S (1999): Proportions of various endocrine cells in the pancreatic ıslets of wood mice (Apodemus speciosus). Anat Histol Embryol, 28, 13– 16.
Abstract
Bu çalışmada, Ankara tavşanlarının (10 adet, sağlıklı ve erişkin) endokrin pankreası histokimyasal ve elektron mikroskobik olarak incelendi. Tavşanlardan alınan pankreas örnekleriBouin, Carnoy ve gluteraldehit-paraformaldehit solüsyonlarında tespit edildi. Aldehit fuksin light green orange G (AF) ile histokimyasal olarak boyanan pankreas kesitlerinde 2 tip endokrin hücre belirlenmesine rağmen, elektron mikroskobik incelemelerde endokrin adacıkların A, B ve D hücrelerinden oluştuğu gözlendi.Langerhans adacıklarının merkezinde mor-menekşe boyanan çok sayıda B hücrelerinin ve periferinde sarımsı-yeşil boyanan az sayıda A hücrelerinin bulunduğu saptandı. Elektron mikroskobik olarak, A hücrelerinin salgı granülü genellikle yuvarlak ve elektron çok yoğun iken, B hücrelerinin sitoplazmasında salgı içeriği ile salgı membranı arasında belirgin boşluk bulunan elektron çok yoğun ve elektron az yoğun granüller tespit edildi. D hücrelerinin de elektron çok yoğun ve elektron az yoğun materyale sahip yuvarlak 2 çeşit granül içerdiği ancak, bu granüllerin A ve B hücrelerinin granüllerinden daha küçük olduğu gözlendi. Bu çalışmanın sonucunda, Ankara tavşanı endokrin pankreasının çok sayıda B hücreleri, az sayıda A hücreleri ve çok az sayıda da D hücrelerinden oluştuğu saptandı
References
- Adeghate E, Ponery AS (2002): Ghrelin stimulates insulin secretion from the pancreas of normal and diabetic rats. J Neuroendocrinol, 14, 555– 560.
- Ali SS, Ali MM, Hering BJ, Bretzel RG, Federlin K (1991): Light- and electron-microscopic studies on isolated bovine islets of Langerhans. Anat Anz, 173, 73– 80.
- Aluments J, Sundler F, Hakånson R (1977): Distribution, ontogeny and ultrastructure of somatostatin immunreactive cells in the pancreas and gut. Cell Tissue Res, 185, 465– 479.
- Bacha WJ, Wood LM (1990): Color Atlas of Veterinary Histology. Lea and Febiger, Philadelphia & London
- Bancroft JD, Cook HC (1984): Manual of Histological Techniques. Churchill Livingstone Medical Division Longman Group Limited, UK.
- Baskin DG, Gorray KC, Fujimoto WY (1984): Immunocytochemical identification of cells containing insulin, glucagon, somatostatin, and pancreatic polypeptide in the islets of Langerhans of the guinea pig pancreas with light and electron microscopy. Anat Rec, 208, 567– 578.
- Bencosme SA, Pease DC (1958): Electron microscopy of the pancreatic islets. Endocrinology, 63, 1– 13.
- Bertelli E, Regoli M, Bastianini A (1994): Endocrine tissue associated with the pancreatic ductal system: a light and electron microscopic study of the adult rat pancreas with special reference to a new endocrine arrangement. Anat Rec, 239, 71– 78.
- Bloom W, Fawcett DW (1994): A Textbook of Histology. Twelfth Ed. Chapman and Hall, Sounders Company, Philadelphia, London, Toronto.
- Böck P, Moneim M, Egerbacher M (1997): Development of pancreas. Microsc Res Techn, 37, 374– 383.
- Cowap J (1985): The first appearence of endocrine cells in the splenic lobe of the embriyonic chick pancreas. Gen Comp Endocrin, 60, 131– 137.
- Culling CFA, Allison RT, Barr WT (1985): Cellular Pathology Technique. Fourth Ed. Butterworth & Co. Ltd. London.
- Dellmann HD, Eurell J (1998): Textbook of Veterinary Histology. 5nd. Ed. Williams & Wilkins A Waverly Company, Pennsylvania, USA
- El-Naggar MM (2000): Ultrastructural immunogold study on the various cell types of cultured pancreatic islets of adult rats. Folia Morphol, 59, 253– 262.
- Greider MH, Gersell DJ, Gingerich RL (1978): Ultrastructural localization of pancreatic polypeptide in the F cell of the dog pancreas. J Histochem Cytochem, 26, 1103– 1108.
- Gomez–Acebo J, Parrilla R, R-Candela JL (1968): Fine structure of the A and D cells of the rabit endocrine pancreas in vivo and incubated in vitro. I. mechanism of secretion of the a cells. J Cell Biol, 36, 33– 44.
- Helmstaedter V, Feurle GE, Forssmann WG (1976): Insulin-, glucagon-, and somatostatin-immunoreactive endocrine cells in the equine pancreas. Cell Tissue Res, 172, 447– 454.
- Kaung CH (1985): Electron microscopic immunocytochemical localization of glucagon and pancreatic polipeptide in rat pancreas: characterization of a population of islet cells containing both peptides. Anat Rec, 212, 292– 300.
- Karnovsky MJ (1965): Formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol, 27, 137A– 138A.
- Lacy PE (1957): Electron microscopic identification of different cell types in the islets of Langerhans of the guinea pig, rat, rabbit and dog. Anat Rec, 128, 255– 267.
- Larsson LI (1998): On the development of the islets of Langerhans. Microsc Res Tech, 43, 284– 291.
- Liman N (1991): Sığır, Koyun ve Keçi’nin Pankreasında A ve B Hücreleri Üzerine Morfolojik çalışmalar. Doktora Tezi, Ankara Üniv. Sağ. Bil. Ens. Ankara.
- Prado CL, Pugh-Bernard AE, Elghazi L, Sosa-Pineda B, Sussel L (2004): Ghrelin cells replace insulin- producing B cells in two mouse models of pancreas development. Proc Natl Acad Sci, 101, 4679– 4684.
- Reddy S, Elliott RB (1985): Insulin, glucagon, pancreatic polypeptide hormone and somatostatin in the goat pancreas: demonstration by immunocytochemistry. Ausr J Biol Sci, 38, 59– 66.
- Slack JMW (1995): Developmental biology of the pancreas. Development, 121, 1569–1580.
- Şimşek N, Özudoğru Z, Alabay B (2008): Immunohistochemical studies on the splenic lobe of the pancreas in young japanese quails (Coturnix c. japonica), DTW, 115, 189– 193.
- Veneable JH, Coggeshall R (1965): A simplified lead citrate stain for use in electron microscopy. J Cell Biol, 25, 407– 408.
- Wolfe-Coote SA, Louw J, Woodroof CW, Heydenrych JJ, Du Toit DF (1998): Induction of cell proliferation an differentiation in the pancreas of the adult Vervet Monkey (Cercopithecus Aethiops). Pancreas, 16, 129– 133.
- Young B, Heath JW (2000): Wheater’s Functional Histology. Fourth Ed. Churchill Livingstone, Sydney, Toronto.
- Yukawa M, Takeuchi T, Watanabe T, Kitamura S (1999): Proportions of various endocrine cells in the pancreatic ıslets of wood mice (Apodemus speciosus). Anat Histol Embryol, 28, 13– 16.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Other ID | JA48JV27RG |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 1, 2010 |
| Published in Issue | Year 2010Volume: 57 Issue: 1 |