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Isolation and identification of motile Aeromonas spp. in turkey meat

Year 2009, Volume: 56 Issue: 2, 95 - 98, 01.06.2009

Cetin Koca Belgin Sarımehmetoglu

https://doi.org/10.1501/Vetfak_0000002170

Abstract

In this study a total of 80 packaged turkey meat samples purchased from different supermarkets in Ankara including 40 leg and 40 breast meat were analysed for the presence of motile Aeromonas species. Totally 43 of 80 turkey meat samples (53.75 %) were determined as positive for motile Aeromonas spp. The distribution of the isolat were 62.5 %, 45 % in leg and breast samples, respectively. Also it is found that two different motile Aeromonas spp. were isolated in same breast samples; in 2 of the samples both A. hydrophila and A. sobria (11.1 %), in 1 of the samples A. hydrophila and A. caviae (5.6 %) and in 1 of the samples A. caviae and A. sobria were isolated (5.6 %). A. hydrophila was isolated as the most prevalent species from all of the positive samples. As a result, turkey meat that analyzed in this study were found to be contaminated with a high level of motile Aeromonas spp., and this is a potential risk for public health

Keywords

Aeromonas hydrophila motile Aeromonas spp. turkey meat

References

  • Abeyta CJr, Charles AK, Wekell MM, Sullivan JJ, Stelma GN (1986): Recovery of Aeromonas hydrophila from oysters implicated in an outbreak of foodborne illness. J Food Protect, 49, 643-646.
  • Abeyta C, Kaysner CA, Wekell MM, Stott RF (1990): Incidence of motile Aeromonas from United States west coast shellfish growing estuaries. J Food Protect, 53, 849- 852.
  • Akan M (1993): Hayvanlardan ve Çevresel Kaynaklardan İzole Edilen Hareketli Aeromonas Türlerinin Biyokim- yasal, Toksijenik, Enzimatik ve Yüzey Özellikleri. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi.
  • Akan M, Eyigor A, Diker KS (1998): Motile Aeromonads in the feces and carcasses of broiler chickens in Turkey. J Food Protect, 61, 113-115.
  • Albert JM, Ansaruzzaman M, Talukder KA, Chopra AK, Kuhn I (2000): Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. J Clin Microbiol, 38, 3785-3790.
  • Anon (1991): Aeromonas hydrophila Foodborne Pathogenic Microorganisms and Natural Toxins Handbook. Erişim: [http://vm.cfsan.fda.gov/~mow/chap17.html]. 20 December 2005.
  • Anon (2000): CCFRA Microbiological Methods Manual. In: Compendium of Microbiological Methods for the Analysis of Food and Agricultural Products. Published by AOAC International.
  • Barnhart HM, Pancorbo OC, Dreesen DW, Shotts JrEB (1989): Recovery of Aeromonas hydrophila from carcasses and processing water in a broiler processing operation. J Food Protect, 52, 646-649.
  • Cahill MM (1990): Virulance factors in motile Aeromonas species. J Appl Bacteriol, 69, 1-16.
  • Doherty A, Sheridan JJ, Alien P, Mcdowell DA, Blair IS, Harrington D (1996): Survival and growth of Aeromonas hydrophila on modified atmosphere packaged normal and high pH lamb. Int J Food Microbiol, 28, 379.
  • Eley A, Geary I, Wilcox MH (1993): Growth of Aeromonas spp. at 4°C and related toxin production. Lett Appl Microbiol, 16, 36-39.
  • Gavriel AA, Landre JRB, Lamb AJ (1998): Incidence of mesophilic Aeromonas within a public drinking water supply in north-east Scotland. J Appl Microbiol, 84, 383- 392.
  • Gold WL, Salit IE (1993): Aeromonas hydrophila infections of skin and soft tissue: report of 11 cases and review. Clin Infect Dis, 16, 69-74.
  • Gonzalez CJ, Lopez-Diaz TM, Garcia-Lopez ML, Prieto M, Otero A (1999): Bacterial microflora of wild brown trout, wild pike, and aquacultured rainbow trout. J Food Protect, 62, 1270-1277.
  • Gonzalez CJ, Santos JA, Gaecia-Lopez ML, Gonzalez N, Otero A (2001): Mesophilic aeromonads in wild and aquacultured freshwater fish. J Food Protect, 64, 687-691.
  • Hanninen ML (1993): Occurrence of Aeromonas spp. in samples of ground meat and chicken. J Food Microbiol, 18, 339-342.
  • Hanninen ML, Oivanen P, Hırvela-Koski V (1997): Aeromonas species in fish, fish-eggs, shrimp, and freshwater. Int J Food Microbiol, 34, 17-26.
  • Hudson JA, De Lacy KM (1991): Incidence of motile Aeromonads in New Zeland retail foods. J Food Protect, 54, 695-699.
  • Hudson JA, Mott SJ, Delacy KM, Eldridge AL (1992): Incidence and coincidence of Listeria spp., motile aeromonads and Yersinia enterocolitica on ready to eat fleshfoods. Int J Food Microbiol, 16, 99-108.
  • Janda JM, Abbot SL (1998): Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations and unanswered questions. Clin Infect Dis, 27, 332-334.
  • Janda JM, Abbot SL (1999): Unusual foodborne pathogens: Listeria monocytogenes, Aeromonas, Plesiomonas and Edwarsiella species. Clin Lab Med, 19, 553-582.
  • Joseph SW, Carnahan A (1994): The isolation, identification and systematics of the motile Aeromonas species. Ann Rev Fish Dis, 4, 315-343.
  • Kirov SM (1993): The public health significance of Aeromonas spp. in foods: a review. J Food Microbiol, 20, 179-198.
  • Krovacek K, Paris A, Mansson I (1991): Growth of and toxin production by Aeromonas hydrophila and Aeromonas sobria at low temperatures. Int J Food Microbiol, 13, 165-176.
  • Kuhn I, Albert JM, Ansaruzzaman M, Bhuiyan NA, Alabi SA, Islam MS, Neogi PKB, Huys G, Janssen P, Kersters K, Mollby R (1997): Characterization of Aeromonas spp. isolated from humans with diarrhea, from healthy controls and from surface water in Bangladesh. J Clin Microbiol, 35, 369-373.
  • Kuplulu O, Sarimehmetoglu B, Kasımoglu A (2000): Sığır Kıymalarından hareketli Aeromonas türlerinin izolasyon ve identifikasyonu. Turk J Vet Anim Sci, 24, 423-428.
  • Merino S, Aguilar A, Nogueras MM, Swift M Regue S, Tomas JM (1999): Cloning, sequencing, and role in virulence of two phospholipases (Al and C) from mesophilic Aeromonas sp. se-rogroup O:34. Infect Immun, 67, 4008-4013.
  • Nedoluha PC, Westhoff D (1993): Microbiological flora of aquacultured hybrid striped bass. J Food Protect, 56, 1054-1060.
  • Palumbo SA, Maxino F, Williams AC, Buchanan RL, Thayer DW (1985): Starch-Ampicillin agar for the quantitative detection of Aeromonas hydrophila. Appl Environ Microbiol, 50, 1027-1030.
  • Palumbo S, Abeyta C, Stelma G (1992): Chapter 30: Aeromonas hydrophila Group. In: Compendium of Methods for the Microbiological Examination of Foods. Third edition, Ed.: C. Vanderzant, D.F. Splittstoesser. APHA, Washington D.C., p.: 497-515
  • Pavan ME, Abbott SL, Zorzopulos J, Janda JM (2000): Aeromonas salmonicida subsp. pectinolytica subsp nov., a new pectinase positive subspecies isolated from a heavily polluted river. Int J System Evo Microbiol, 50, 1119-1924.
  • Roberts TA, Baird-Parker AC, Tompkin RB (1996): Aeromonas. In: Microorganisms in Foods Characteristics of Microbial Pathogens. Vol. 5. London. ICMSF, Blakie Academic and Professional. p.: 5-19
  • Saad SMI, Laria ST, Furlanetio SMP (1995): Motile Aeromonas spp. in retail vegetables from Sao Paulo, Brazil. Rev Microbiol, 26, 22-27.
  • Santos JA, Lopez-Diaz TM, Garcia-Fernandez MC, Garcia-Lopez ML, Otero A (1996): Villalon, a fresh ewe’s milk Spanish cheese as a source of potentially pathogenic Aeromonas strains. J Food Protect, 59, 1288- 1291.
  • Sarimehmetoglu B, Kuplulu O, Kaymaz S (1998): Ankara’da tüketime sunulan pastörize sütlerden hareketli Aeromonas türlerinin izolasyon ve identifikasyonu. Gıda, 23, 141-145
  • Sarimehmetoglu B, Kuplulu O (2001): Isolation and identification of motile Aeromonas species form chicken. DTW, 108, 465-467.
  • Singh U (1997): Isolation and identification of Aeromonas spp. from ground meats in Eastern Canada. J Food Protect, 60, 125-130.
  • Stelma GN (1988): Virulence factors assocaited with pathogenicity of Aeromonas isolates. J Food Safety, 9, 1-4.
  • Ternstrom A, Molin G (1987): Incidence of potential pathogens on raw pork, beef and chicken in Sweden, with special reference to Erysipelothrix rhusiopathiae. J Food Protect, 50, 141-146.
  • Wang C, Silva JL (1999): Prevalence and characteristics of Aeromonas species isolated from processed channel catfish. J Food Protect, 62, 30-34.
  • Yucel N, Erdem B (2004): The isolation and identification of motile Aeromonas spp. from meats in Ankara, Turkey. Indian Vet J, 81, 967-970.

Hindi etlerinden hareketli Aeromonas türlerinin izolasyon ve identifikasyonu

Year 2009, Volume: 56 Issue: 2, 95 - 98, 01.06.2009

Cetin Koca Belgin Sarımehmetoglu

https://doi.org/10.1501/Vetfak_0000002170

Abstract

Bu çalışmada Ankara’nın değişik semtlerindeki marketlerden alınan paketlenmiş 40 göğüs kuşbaşı ve 40 but kuşbaşı örneklerinden oluşan toplam 80 hindi eti Aeromonas spp. varlığı yönünden incelenmiştir. Çalışma kapsamında incelenen, 80 hindi eti örneğinin 43’ünden (% 53.75), göğüs ve but kuşbaşı örneklerinden sırasıyla % 45 ve % 62.5 düzeyinde hareketli Aeromonas türleri izole edilmiştir. Ayrıca aynı göğüs eti örneklerinden iki farklı hareketli Aeromonas spp. izole edilmiştir. Buna göre iki (% 11.1) örnekten A. hydrophila ve A. sobria, bir (% 5.6) örnekten A. hydrophila ve A. caviae ve bir (% 5.6) örnekten A. caviae ve A. sobria izole edilmiştir. Örneklerden izole edilen en yaygın tür A. hydrophila olarak saptanmıştır. Sonuç olarak, çalışma kapsamında incelenen hindi etlerinin büyük bölümünün hareketli Aeromonas’larla kontamine olduğu saptanmış olup, bu durum halk sağlığı açısından potansiyel bir risk oluşturmaktadır

Keywords

Aeromonas hydrophila hareketli Aeromonas spp. hindi eti

References

  • Abeyta CJr, Charles AK, Wekell MM, Sullivan JJ, Stelma GN (1986): Recovery of Aeromonas hydrophila from oysters implicated in an outbreak of foodborne illness. J Food Protect, 49, 643-646.
  • Abeyta C, Kaysner CA, Wekell MM, Stott RF (1990): Incidence of motile Aeromonas from United States west coast shellfish growing estuaries. J Food Protect, 53, 849- 852.
  • Akan M (1993): Hayvanlardan ve Çevresel Kaynaklardan İzole Edilen Hareketli Aeromonas Türlerinin Biyokim- yasal, Toksijenik, Enzimatik ve Yüzey Özellikleri. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Doktora Tezi.
  • Akan M, Eyigor A, Diker KS (1998): Motile Aeromonads in the feces and carcasses of broiler chickens in Turkey. J Food Protect, 61, 113-115.
  • Albert JM, Ansaruzzaman M, Talukder KA, Chopra AK, Kuhn I (2000): Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. J Clin Microbiol, 38, 3785-3790.
  • Anon (1991): Aeromonas hydrophila Foodborne Pathogenic Microorganisms and Natural Toxins Handbook. Erişim: [http://vm.cfsan.fda.gov/~mow/chap17.html]. 20 December 2005.
  • Anon (2000): CCFRA Microbiological Methods Manual. In: Compendium of Microbiological Methods for the Analysis of Food and Agricultural Products. Published by AOAC International.
  • Barnhart HM, Pancorbo OC, Dreesen DW, Shotts JrEB (1989): Recovery of Aeromonas hydrophila from carcasses and processing water in a broiler processing operation. J Food Protect, 52, 646-649.
  • Cahill MM (1990): Virulance factors in motile Aeromonas species. J Appl Bacteriol, 69, 1-16.
  • Doherty A, Sheridan JJ, Alien P, Mcdowell DA, Blair IS, Harrington D (1996): Survival and growth of Aeromonas hydrophila on modified atmosphere packaged normal and high pH lamb. Int J Food Microbiol, 28, 379.
  • Eley A, Geary I, Wilcox MH (1993): Growth of Aeromonas spp. at 4°C and related toxin production. Lett Appl Microbiol, 16, 36-39.
  • Gavriel AA, Landre JRB, Lamb AJ (1998): Incidence of mesophilic Aeromonas within a public drinking water supply in north-east Scotland. J Appl Microbiol, 84, 383- 392.
  • Gold WL, Salit IE (1993): Aeromonas hydrophila infections of skin and soft tissue: report of 11 cases and review. Clin Infect Dis, 16, 69-74.
  • Gonzalez CJ, Lopez-Diaz TM, Garcia-Lopez ML, Prieto M, Otero A (1999): Bacterial microflora of wild brown trout, wild pike, and aquacultured rainbow trout. J Food Protect, 62, 1270-1277.
  • Gonzalez CJ, Santos JA, Gaecia-Lopez ML, Gonzalez N, Otero A (2001): Mesophilic aeromonads in wild and aquacultured freshwater fish. J Food Protect, 64, 687-691.
  • Hanninen ML (1993): Occurrence of Aeromonas spp. in samples of ground meat and chicken. J Food Microbiol, 18, 339-342.
  • Hanninen ML, Oivanen P, Hırvela-Koski V (1997): Aeromonas species in fish, fish-eggs, shrimp, and freshwater. Int J Food Microbiol, 34, 17-26.
  • Hudson JA, De Lacy KM (1991): Incidence of motile Aeromonads in New Zeland retail foods. J Food Protect, 54, 695-699.
  • Hudson JA, Mott SJ, Delacy KM, Eldridge AL (1992): Incidence and coincidence of Listeria spp., motile aeromonads and Yersinia enterocolitica on ready to eat fleshfoods. Int J Food Microbiol, 16, 99-108.
  • Janda JM, Abbot SL (1998): Evolving concepts regarding the genus Aeromonas: an expanding panorama of species, disease presentations and unanswered questions. Clin Infect Dis, 27, 332-334.
  • Janda JM, Abbot SL (1999): Unusual foodborne pathogens: Listeria monocytogenes, Aeromonas, Plesiomonas and Edwarsiella species. Clin Lab Med, 19, 553-582.
  • Joseph SW, Carnahan A (1994): The isolation, identification and systematics of the motile Aeromonas species. Ann Rev Fish Dis, 4, 315-343.
  • Kirov SM (1993): The public health significance of Aeromonas spp. in foods: a review. J Food Microbiol, 20, 179-198.
  • Krovacek K, Paris A, Mansson I (1991): Growth of and toxin production by Aeromonas hydrophila and Aeromonas sobria at low temperatures. Int J Food Microbiol, 13, 165-176.
  • Kuhn I, Albert JM, Ansaruzzaman M, Bhuiyan NA, Alabi SA, Islam MS, Neogi PKB, Huys G, Janssen P, Kersters K, Mollby R (1997): Characterization of Aeromonas spp. isolated from humans with diarrhea, from healthy controls and from surface water in Bangladesh. J Clin Microbiol, 35, 369-373.
  • Kuplulu O, Sarimehmetoglu B, Kasımoglu A (2000): Sığır Kıymalarından hareketli Aeromonas türlerinin izolasyon ve identifikasyonu. Turk J Vet Anim Sci, 24, 423-428.
  • Merino S, Aguilar A, Nogueras MM, Swift M Regue S, Tomas JM (1999): Cloning, sequencing, and role in virulence of two phospholipases (Al and C) from mesophilic Aeromonas sp. se-rogroup O:34. Infect Immun, 67, 4008-4013.
  • Nedoluha PC, Westhoff D (1993): Microbiological flora of aquacultured hybrid striped bass. J Food Protect, 56, 1054-1060.
  • Palumbo SA, Maxino F, Williams AC, Buchanan RL, Thayer DW (1985): Starch-Ampicillin agar for the quantitative detection of Aeromonas hydrophila. Appl Environ Microbiol, 50, 1027-1030.
  • Palumbo S, Abeyta C, Stelma G (1992): Chapter 30: Aeromonas hydrophila Group. In: Compendium of Methods for the Microbiological Examination of Foods. Third edition, Ed.: C. Vanderzant, D.F. Splittstoesser. APHA, Washington D.C., p.: 497-515
  • Pavan ME, Abbott SL, Zorzopulos J, Janda JM (2000): Aeromonas salmonicida subsp. pectinolytica subsp nov., a new pectinase positive subspecies isolated from a heavily polluted river. Int J System Evo Microbiol, 50, 1119-1924.
  • Roberts TA, Baird-Parker AC, Tompkin RB (1996): Aeromonas. In: Microorganisms in Foods Characteristics of Microbial Pathogens. Vol. 5. London. ICMSF, Blakie Academic and Professional. p.: 5-19
  • Saad SMI, Laria ST, Furlanetio SMP (1995): Motile Aeromonas spp. in retail vegetables from Sao Paulo, Brazil. Rev Microbiol, 26, 22-27.
  • Santos JA, Lopez-Diaz TM, Garcia-Fernandez MC, Garcia-Lopez ML, Otero A (1996): Villalon, a fresh ewe’s milk Spanish cheese as a source of potentially pathogenic Aeromonas strains. J Food Protect, 59, 1288- 1291.
  • Sarimehmetoglu B, Kuplulu O, Kaymaz S (1998): Ankara’da tüketime sunulan pastörize sütlerden hareketli Aeromonas türlerinin izolasyon ve identifikasyonu. Gıda, 23, 141-145
  • Sarimehmetoglu B, Kuplulu O (2001): Isolation and identification of motile Aeromonas species form chicken. DTW, 108, 465-467.
  • Singh U (1997): Isolation and identification of Aeromonas spp. from ground meats in Eastern Canada. J Food Protect, 60, 125-130.
  • Stelma GN (1988): Virulence factors assocaited with pathogenicity of Aeromonas isolates. J Food Safety, 9, 1-4.
  • Ternstrom A, Molin G (1987): Incidence of potential pathogens on raw pork, beef and chicken in Sweden, with special reference to Erysipelothrix rhusiopathiae. J Food Protect, 50, 141-146.
  • Wang C, Silva JL (1999): Prevalence and characteristics of Aeromonas species isolated from processed channel catfish. J Food Protect, 62, 30-34.
  • Yucel N, Erdem B (2004): The isolation and identification of motile Aeromonas spp. from meats in Ankara, Turkey. Indian Vet J, 81, 967-970.
There are 41 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Other ID JA22NC98HM
Journal Section Research Article
Authors

Cetin Koca

Belgin Sarımehmetoglu

Publication Date June 1, 2009
Published in Issue Year 2009Volume: 56 Issue: 2

Cite

APA Koca, C., & Sarımehmetoglu, B. (2009). Isolation and identification of motile Aeromonas spp. in turkey meat. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 56(2), 95-98. https://doi.org/10.1501/Vetfak_0000002170
AMA Koca C, Sarımehmetoglu B. Isolation and identification of motile Aeromonas spp. in turkey meat. Ankara Univ Vet Fak Derg. June 2009;56(2):95-98. doi:10.1501/Vetfak_0000002170
Chicago Koca, Cetin, and Belgin Sarımehmetoglu. “Isolation and Identification of Motile Aeromonas Spp. In Turkey Meat”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56, no. 2 (June 2009): 95-98. https://doi.org/10.1501/Vetfak_0000002170.
EndNote Koca C, Sarımehmetoglu B (June 1, 2009) Isolation and identification of motile Aeromonas spp. in turkey meat. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56 2 95–98.
IEEE C. Koca and B. Sarımehmetoglu, “Isolation and identification of motile Aeromonas spp. in turkey meat”, Ankara Univ Vet Fak Derg, vol. 56, no. 2, pp. 95–98, 2009, doi: 10.1501/Vetfak_0000002170.
ISNAD Koca, Cetin - Sarımehmetoglu, Belgin. “Isolation and Identification of Motile Aeromonas Spp. In Turkey Meat”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 56/2 (June 2009), 95-98. https://doi.org/10.1501/Vetfak_0000002170.
JAMA Koca C, Sarımehmetoglu B. Isolation and identification of motile Aeromonas spp. in turkey meat. Ankara Univ Vet Fak Derg. 2009;56:95–98.
MLA Koca, Cetin and Belgin Sarımehmetoglu. “Isolation and Identification of Motile Aeromonas Spp. In Turkey Meat”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 56, no. 2, 2009, pp. 95-98, doi:10.1501/Vetfak_0000002170.
Vancouver Koca C, Sarımehmetoglu B. Isolation and identification of motile Aeromonas spp. in turkey meat. Ankara Univ Vet Fak Derg. 2009;56(2):95-8.