Letter to Editor
Investigation of serum nitric oxide and malondialdehyde levels in cattle infected with Brucella abortus
Abstract
The objective of this study was to determine serum nitric oxide (NO) and malondialdehyde (MDA) levels in cattle infected with Brucella abortus (B. abortus). Study cohort was comprised of 30 cattle aged between 2-3 years; 20 infected with B. abortus and 10 healthy cattle. B. abortus infection was determined in the animals by Serum Agglutination Test and Multiplex PCR assay. Serum NO level was determined by enzymatic Greiss reaction and MDA level by Yoshioka method. Mean serum NO and MDA levels of cases with confirmed B. abortus were 58.20 ± 6.50 µmol/L and 2.25 ± 0.10 nmol/ml, respectively whereas those of the controls were 38.07 ± 2.40 µmol/L and 1.74 ± 0.25 nmol/ml, respectively. We found that serum NO and MDA levels in infected cattle were significantly higher than healthy control cattle (p < 0.05 and p < 0.01, respectively). In conclusion, the increase in NO level in cattle infected with B. abortus is a result of stimulation of NO synthesis in macrophages by bacterial lipopolysaccharides, and the increase in MDA level is a result of excessive radical production due to brucellosis acting upon membrane lipids and can be used as an indicator of tissue damage
References
- Akaike T, Maeda H (2000): Nitric oxide and virus infection. Immunology, 101, 300-308.
- Atroshi F, Parantaınen J, Sankari S, Jarvinen M, Lindberg LA, Saloniemi H (1996): Changes in inflammation-related blood constituents of mastitic cows. Vet Res, 27, 125–132.
- Aydın N, Bisping W, Akay O, İzgür M, Kirpal G (1988): Untersuchungen zum vorkommen boviner brucellose in derb Türkei bewertung der immunisierengen wirkung zweier vakzien. Berlin Münch Tieraerztliche Wschrft, 101,109–113.
- Aydın N, Arda M, Akay Ö, İzgür M, Ayhan H, Esendal Ö, Aydın F (1990): Atık fetuslardan izole edilen brucella suşlarının ko-aglütinasyon testi ile identifikasyonları. Ankara Üniv Vet Fak Derg, 37, 348–358.
- Bouchard L, Blais S, Desrosiers C, Zhao X, Lacasse P (1999): Nitric oxide production during endotoxin-induced mastitis in the cow. J Dairy Sci, 82, 2574-2581.
- Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus bv. 1, 2 and 4, Brucell melitensis, Brucella ovis and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660-2666.
- Burgner D, Rockett K, Kwiatkowski D (1999): Nitric oxide and infectious diseases. Arch Dis Child, 81, 185– 188.
- Cortas NK, Wakid NW (1990): Determination of inorganic nitrate in serum and urine by a kinetic cadmium reduction method. Clin Chem, 36, 1440-1443.
- Czuczejko J, Zachara BA, Staubach-Topczewska E, Halota W, Kedziora J (2003): Selenium, glutathione and glutathione peroxidases in blood of patients with chronic liver diseases. Acta Biochim Pol, 50, 1147-1154.
- Demir S, Yılmaz M, Köseoğlu M, Akalın N, Aslan D, Aydın A (2003): Role of free radicals in peptic ulcer and gastritis. Turk J Gastroenterol, 14, 39-43.
- Fidancı HA, Akın S, Alabay M, Güvener N. (1995): Sığırlarda Brucella abortus’a karşı oluşan antikorları saptamada ELISA ve diğer serolojik tekniklerin karşılaştırılması. Ankara Üniv Vet Fak Derg, 42, 553–557.
- Gross A, Bertholet S, Mauel J, Dornand J (2004): Impairment of Brucella growth in human macrophagic cells that produce nitric oxide. Microb Pathog, 36, 75-82.
- Guarner C, Soriano G, Tomas A, Bulbena O, Novella MT, Balanzo J, Vilardell F, Mourelle M, Moncada S (1993): Increased serum nitrite and nitrate levels in patients with cirrhosis: relationship to endotoxemia. Hepatology, 18, 1139-1143.
- Halliwell B (1994): Free radicals and antioxidants:a personal view. Nutr Rev, 52, 253–265.
- Inal ME, Kabak G, Sunal E (2001): Antioxidant enzyme activities and malondialdehyde levels related to aging. Clinica Chimica Acta, 305, 75-80.
- Izgur M, Akay O, Arda M, Erdeger J (1992): Sığır brucellosisinin teşhisinde EDTA ve 56°C’de aglutinasyon testlerinin kullanılması. Ankara Üniv Vet Fak Derg, 39, 191–200.
- Kandemir O, Eskandari G, Camdeviren H, Sahin E, Kaya A, Atik U (2002): Plasma malondialdehyde and nitrate levels in patients with brucellosis. MEU Tıp Fak Derg, 3, 405–409.
- Kenar B (1990): Konya, Niğde, Nevşehir ve Kayseri illerinde koyun ve sığır brusellozisinin sero-survey epidemiyolojik araştırması. Veterinarium, 1, 34–37.
- Kubes P, Mccafferty, DM (2000): Nitric oxide and intestinal inflammation. Am J Med, 109, 150–158.
- Kuyumcu A, Düzgün AP, Özmen M, Besler T (2004): The role of nitric oxide in trauma and infection. Ulus Travma Derg, 10, 149-159.
- Kwon NS, Stueh DJ, Nathan CF (1991): Inhibition of tumor cell ribonucleotide reductase by macrophage- derived nitric oxide. J Exp Med, 174, 761–767.
- Lowenstein CJ, Dınerman JL, Snyder SH (1994): Nitric oxide: a physiologic messenger. Ann Intern Med, 120, 227–237.
- Madebo T, Lindtjorn B, Aukrust P, Berge RK (2003): Circulating antioxidants and lipid peroxidation products in untreated tuberculosis patients in Ethiopia. Am J Clin Nutr, 78, 117–122.
- Marnett LJ (2002): Oxy radicals, lipid peroxidation and DNA damage. Toxicology, 27, 219-222.
- McCord, JM (2000): The evolution of free radicals and oxidative stress. Am J Med, 108, 652–659.
- Orozco G, Sánchez E, López-Nevot MA, Caballero A, Bravo MJ, Morata P, Colmenero JD, Alonso A, Martin J (2003): Inducible nitric oxide synthase promoter polymorphism in human brucellosis. Microbes Infect, 5, 1165-1169.
- Pernet P, Coudray-Lucas C, Le Boucher J, Schlegel L, Gıboudeau J, Cynober L, Aussel C (1999): Is the 37. You D, Ren X, Xue Y, Luo G, Yang T, Shen J (2003): A L-arginine-nitric oxide pathway involved in endotoxemia- induced muscular hypercatabolism in rats. Metabolism, 48, 190–193.
- Pfister H, Remer KA, Brcic M, Fatzer R, Christen S, Leib S, Jungi TW (2002): Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol, 39, 190-199.
- Rao CR (1973): Linear Statistical Inference and Its Applications. John&Sons, New York.
- Safarian MD, Karagezian KG, Karapetian ET, Avanesian NA (1990): The efficacy of antioxidant therapy in patients with tuberculosis of the lungs and the correction of lipid peroxidation processes. Probl Tuberk, 5, 40-44.
- Taylor-Robinson, AW, Severn AW, Phillips RS (1996): Kinetics of nitric oxide production during infection and reinfection of mice with Plasmodium chabaudi. Parasite Immunol, 18, 425–430.
- Urrutia LL,Alonso A, Nieto ML, Bayon Y, Orduna A, Crespo MS (2000): Lipopolysaccharides of Brucella abortus and Brucella melitensis induce nitric oxide synthesis in rat peritoneal macrophages. Infect Immun, 68, 1740–1745.
- Uysal Y (1998): Sığır ve koyun atıklarında brucellaların önemi. Ulusal Sığır ve Koyun Yavru Atma Sempozyumu 06–08 Ekim 1998. Pendik Hayvan Hastalıkları Araştırma Enstitüsü, İstanbul.
- Wang M, Qureshi N, Soeurt N, Splitter G (2001): High levels of nitric oxide production decrease early but increase late survival of Brucella abortus in macrophages. Microb Pathog, 31, 221-230.
- Wang Y, Li Q, Hu S (2005): A multiwall carbon nanotubes film-modified carbon fiber ultramicroelectrode for the determination of nitric oxide radical in liver mitochondria. Bioelectrochemistry, 65, 135-142.
- Yoshioka T, Kawada K, Shimada T, Mori M (1979): Lipid peroxidation in maternal and cord blood and protective mechanism aganist actived oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376. selenium-containing single-chain abzyme with potent antioxidant activity. Eur J Biochem, 270, 4326-4331.
Brucella abortus ile enfekte sığırlarda serum nitrik oksit ve malondialdehit düzeylerinin araştırılması
Abstract
Bu çalışmanın amacı Brucella abortus (B. abortus) ile enfekte sığırlarda serum nitrik oksit (NO) ve malondialdehit (MDA) düzeylerini belirlemektir. Çalışmanın materyalini 20 adet B. abortus ile enfekte ve 10 adet sağlıklı olmak üzere 2-3 yaşlarında 30 adet sığır oluşturdu. B. abortus infeksiyonunun tespiti Serum Aglutinasyon Testi ve Multipleks PCR ile gerçekleştirildi. Serum NO düzeyi enzimatik Greiss reaksiyonu ile MDA düzeyi Yoshioka metodu ile belirlendi. B. abortus ile enfekte sığırlarda serum NO ve MDA düzeyleri sırası ile 58.20 ± 6.50 µmol/L ve 2.25 ± 0.10 nmol/ml, kontrol sığırlarda ise 38.07 ± 2.40 µmol/L ve 1.74 ± 0.25 nmol/ml bulundu. Enfekte sığırlarda serum NO ve MDA düzeylerinin sağlıklı kontrollerden istatistik olarak önemli oranda yüksek olduğu belirlendi (sırası ile, p < 0.05 ve p < 0.01). Sonuç olarak, NO düzeyindeki artışın bakteriyel lipopolisakkaridler ile makrofajlardan NO sentezinin uyarılmasının ve MDA düzeyindeki artışın da brusellozis enfeksiyonunun membran lipidlerini etkileyerek aşırı radikal üretimine neden olmasının sonucu olabileceği ve doku hasarının bir göstergesi olarak kullanılabileceği kanaatine varıldı
Keywords
References
- Akaike T, Maeda H (2000): Nitric oxide and virus infection. Immunology, 101, 300-308.
- Atroshi F, Parantaınen J, Sankari S, Jarvinen M, Lindberg LA, Saloniemi H (1996): Changes in inflammation-related blood constituents of mastitic cows. Vet Res, 27, 125–132.
- Aydın N, Bisping W, Akay O, İzgür M, Kirpal G (1988): Untersuchungen zum vorkommen boviner brucellose in derb Türkei bewertung der immunisierengen wirkung zweier vakzien. Berlin Münch Tieraerztliche Wschrft, 101,109–113.
- Aydın N, Arda M, Akay Ö, İzgür M, Ayhan H, Esendal Ö, Aydın F (1990): Atık fetuslardan izole edilen brucella suşlarının ko-aglütinasyon testi ile identifikasyonları. Ankara Üniv Vet Fak Derg, 37, 348–358.
- Bouchard L, Blais S, Desrosiers C, Zhao X, Lacasse P (1999): Nitric oxide production during endotoxin-induced mastitis in the cow. J Dairy Sci, 82, 2574-2581.
- Bricker BJ, Halling SM (1994): Differentiation of Brucella abortus bv. 1, 2 and 4, Brucell melitensis, Brucella ovis and Brucella suis bv. 1 by PCR. J Clin Microbiol, 32, 2660-2666.
- Burgner D, Rockett K, Kwiatkowski D (1999): Nitric oxide and infectious diseases. Arch Dis Child, 81, 185– 188.
- Cortas NK, Wakid NW (1990): Determination of inorganic nitrate in serum and urine by a kinetic cadmium reduction method. Clin Chem, 36, 1440-1443.
- Czuczejko J, Zachara BA, Staubach-Topczewska E, Halota W, Kedziora J (2003): Selenium, glutathione and glutathione peroxidases in blood of patients with chronic liver diseases. Acta Biochim Pol, 50, 1147-1154.
- Demir S, Yılmaz M, Köseoğlu M, Akalın N, Aslan D, Aydın A (2003): Role of free radicals in peptic ulcer and gastritis. Turk J Gastroenterol, 14, 39-43.
- Fidancı HA, Akın S, Alabay M, Güvener N. (1995): Sığırlarda Brucella abortus’a karşı oluşan antikorları saptamada ELISA ve diğer serolojik tekniklerin karşılaştırılması. Ankara Üniv Vet Fak Derg, 42, 553–557.
- Gross A, Bertholet S, Mauel J, Dornand J (2004): Impairment of Brucella growth in human macrophagic cells that produce nitric oxide. Microb Pathog, 36, 75-82.
- Guarner C, Soriano G, Tomas A, Bulbena O, Novella MT, Balanzo J, Vilardell F, Mourelle M, Moncada S (1993): Increased serum nitrite and nitrate levels in patients with cirrhosis: relationship to endotoxemia. Hepatology, 18, 1139-1143.
- Halliwell B (1994): Free radicals and antioxidants:a personal view. Nutr Rev, 52, 253–265.
- Inal ME, Kabak G, Sunal E (2001): Antioxidant enzyme activities and malondialdehyde levels related to aging. Clinica Chimica Acta, 305, 75-80.
- Izgur M, Akay O, Arda M, Erdeger J (1992): Sığır brucellosisinin teşhisinde EDTA ve 56°C’de aglutinasyon testlerinin kullanılması. Ankara Üniv Vet Fak Derg, 39, 191–200.
- Kandemir O, Eskandari G, Camdeviren H, Sahin E, Kaya A, Atik U (2002): Plasma malondialdehyde and nitrate levels in patients with brucellosis. MEU Tıp Fak Derg, 3, 405–409.
- Kenar B (1990): Konya, Niğde, Nevşehir ve Kayseri illerinde koyun ve sığır brusellozisinin sero-survey epidemiyolojik araştırması. Veterinarium, 1, 34–37.
- Kubes P, Mccafferty, DM (2000): Nitric oxide and intestinal inflammation. Am J Med, 109, 150–158.
- Kuyumcu A, Düzgün AP, Özmen M, Besler T (2004): The role of nitric oxide in trauma and infection. Ulus Travma Derg, 10, 149-159.
- Kwon NS, Stueh DJ, Nathan CF (1991): Inhibition of tumor cell ribonucleotide reductase by macrophage- derived nitric oxide. J Exp Med, 174, 761–767.
- Lowenstein CJ, Dınerman JL, Snyder SH (1994): Nitric oxide: a physiologic messenger. Ann Intern Med, 120, 227–237.
- Madebo T, Lindtjorn B, Aukrust P, Berge RK (2003): Circulating antioxidants and lipid peroxidation products in untreated tuberculosis patients in Ethiopia. Am J Clin Nutr, 78, 117–122.
- Marnett LJ (2002): Oxy radicals, lipid peroxidation and DNA damage. Toxicology, 27, 219-222.
- McCord, JM (2000): The evolution of free radicals and oxidative stress. Am J Med, 108, 652–659.
- Orozco G, Sánchez E, López-Nevot MA, Caballero A, Bravo MJ, Morata P, Colmenero JD, Alonso A, Martin J (2003): Inducible nitric oxide synthase promoter polymorphism in human brucellosis. Microbes Infect, 5, 1165-1169.
- Pernet P, Coudray-Lucas C, Le Boucher J, Schlegel L, Gıboudeau J, Cynober L, Aussel C (1999): Is the 37. You D, Ren X, Xue Y, Luo G, Yang T, Shen J (2003): A L-arginine-nitric oxide pathway involved in endotoxemia- induced muscular hypercatabolism in rats. Metabolism, 48, 190–193.
- Pfister H, Remer KA, Brcic M, Fatzer R, Christen S, Leib S, Jungi TW (2002): Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol, 39, 190-199.
- Rao CR (1973): Linear Statistical Inference and Its Applications. John&Sons, New York.
- Safarian MD, Karagezian KG, Karapetian ET, Avanesian NA (1990): The efficacy of antioxidant therapy in patients with tuberculosis of the lungs and the correction of lipid peroxidation processes. Probl Tuberk, 5, 40-44.
- Taylor-Robinson, AW, Severn AW, Phillips RS (1996): Kinetics of nitric oxide production during infection and reinfection of mice with Plasmodium chabaudi. Parasite Immunol, 18, 425–430.
- Urrutia LL,Alonso A, Nieto ML, Bayon Y, Orduna A, Crespo MS (2000): Lipopolysaccharides of Brucella abortus and Brucella melitensis induce nitric oxide synthesis in rat peritoneal macrophages. Infect Immun, 68, 1740–1745.
- Uysal Y (1998): Sığır ve koyun atıklarında brucellaların önemi. Ulusal Sığır ve Koyun Yavru Atma Sempozyumu 06–08 Ekim 1998. Pendik Hayvan Hastalıkları Araştırma Enstitüsü, İstanbul.
- Wang M, Qureshi N, Soeurt N, Splitter G (2001): High levels of nitric oxide production decrease early but increase late survival of Brucella abortus in macrophages. Microb Pathog, 31, 221-230.
- Wang Y, Li Q, Hu S (2005): A multiwall carbon nanotubes film-modified carbon fiber ultramicroelectrode for the determination of nitric oxide radical in liver mitochondria. Bioelectrochemistry, 65, 135-142.
- Yoshioka T, Kawada K, Shimada T, Mori M (1979): Lipid peroxidation in maternal and cord blood and protective mechanism aganist actived oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376. selenium-containing single-chain abzyme with potent antioxidant activity. Eur J Biochem, 270, 4326-4331.
There are 36 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2007 |
| Published in Issue | Year 2007Volume: 54 Issue: 3 |