The effect of vitamin A added to diet contained aflatoxin on performance, liver and spleen weights andtibia ash in broilers
Abstract
The aim of this study was to determine the effect of vitamin A supplementation on performance, liver and spleen weights, and tibia ash of broilers. A total of 84 one-day-old Ross 308 broiler chicks were randomly assigned to 12 pens each containing seven chicks (4 male, 3 female). Each treatment has 4 replicates. The experiment was conducted with one control and two treatment groups. The chicks in control group were fed a diet without aflatoxin supplementation while the chicks in aflatoxin and aflatoxin+vitamin A groups were fed with the diets 2.5 ppm aflatoxin, and 2.5 ppm aflatoxin + 3866.66 IU vitamin A /kg feed, respectively. The experiment lasted 21 days. In this study, body weight of chicks in aflatoxin and aflatoxin+vitamin A groups were significantly lower (p<0.01) than the chicks in control group. At the end of the experiment, body weight of chicks in control, aflatoxin and aflatoxin+vitamin A groups were found as 480.04 ± 15.76, 362.52 ± 16.06 and 353.04 ± 16.06 g respectively. Supplementation of aflatoxin at the level of 2.5 ppm with and without vitamin A at the level of 3866.66 IU/kg feed to the diets decreased (p<0.001) feed consumption and did not cause a significant difference for feed conversion in all groups. Liver and spleen weights were higher (p<0.01) in aflatoxin and aflatoxin+vitamin A groups than those of the control group. Tibia ash of chicks was higher in control group (p<0.05) than those of aflatoxin group and similar to aflatoxin+vitamin A group
References
- Association of Offical Analytical Chemists (1990): Official Methods of Analysis of the Association of Official Analytical Chemist. 14th ed, Arlington, Virginia.
- Araba M, Wyatt RD (1991): Effects of sodium bentanite, hydrated sodium calcium aluminoslicate (Novasil) and ethacal on aflatoxicosis in broiler cfhicks. Poult Sci, 70, 6.
- Campbell TC, Hayes JR (1976): The role of aflatoxin metabolism in its toxic lesion. Toxicol Appl Pharmacol, 35, 199-222.
- Demet Ö, Oğuz H, Adıgüzel H (1995): Pirinçte aflatoksin üretimi. Vet Bil Derg, 11, 135-140.
- Dickson IR, Walls J, Webb S (1989): A vitamini and bone formation. Different responses to retinol and retinoic acid of chick bone cell in organ culture. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1013, 254-258.
- Edrington TS, Kubena LF, Harvey RB, Rottinghaus GE (1997): Influence of a superactivated charcoal on the toxic effects of aflatoxin or T-2 toxin in growing broilers.
- Ghosh RC, Chauhan HVS, Roy S (1990): Immunosuppression in broilers under experimental aflatoxicosis. Br Vet J, 146, 457-462.
- Harvey RB, Kubena LF, Elissalde MH, Philliıps TD (1993): Efficacy of zeolitic compounds on toxicity of aflatoxin to growing broiler chickens. Avian Dis, 37, 67- 93.
- Hoehler D, Marguardt RR (1996): Influence of vitamin E and C on the toxic effects of ochratoxin A and T-2 toxin in chicks. Poult Sci, 75, 1508-1515.
- Huff V E, Kubena LE, Harvey RB, Corrier DE, Mollenhauer HH (1986): Progression of aflatoxicosis in broiler chickens. Poult Sci, 65, 1891-1899.
- Jindal N, Mahipal SK, Mahajan NK (1994): Toxicity of aflatoxin B1 in broiler chicks and its reduction by activated chacoal, Res In Vet Sci, 56, 37-40.
- Kubena LF, Harvey RB, Bailey RH, Buckley SA, Rottinghaus GE (1998): Effects of a hydrated sodium calcium aluminosilicate T-BindTM on mycotoxicosis in young broiler chickens. Poult Sci, 78, 204-210.
- Kubena LF, Harvey RB, Phillips TD, Corrier DE (1990): Diminition of aflatoxicosis in growing chickens by the dietary addition of anhydrated sodium calcium aluminosilicate. Poult Sci, 69, 727-735.
- Larsson P, Tjalve H (1992): Binding of aflatoxin B1 metabolites in extrahepatic tissues in fetal and infant mice and in adult mice with depletion glutathione levels. Cancer Res, 52, 1267-1277.
- Ledoux DR, Rottinghaus GE, Bermudez AJ, Alanso- Debolt M (1999): Efficacy of hydrated sodium calsium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poult Sci, 78, 204-210.
- Liu XL, Zhou YZ (1989): The effect of aflatoxin B1 on A vitamini status and on microsomal mixed function oxidase in male Mouse. Zhonghua Yu Fang Yi Xue Za Zhi, 23, 218-221.
- Miazzo R, Rosa CA, De Queiroz Carvalho EC, Magnoli C, Chiacchiera SM, Palacio G, Saenz M, Kikot A, Basaldella E, Dalcero A (2000): Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poult Sci, 79, 1-6.
- National Research Council (1994): Nutrient Requirements of Poultry. 9th review. National Academy Press, Washington, DC
- Nyandieka HS, Wakhisi J (1993): The impact of vitamins A, C, E, and selenium compound on prevention of liver cancer in rats. East Afr Med J, 70, 151-153.
- Oğuz H, Kurtoğlu V (2000): Effect of clinoptilolite on fattening performance of broiler chickens during experimental aflatoxicosis. Br Poult Sci, 41, 512-517.
- Oğuz H, Keçeci T, Birdane YO, Önder F, Kurtoğlu V (2000): Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during experimental aflatoxicosis. Res Vet Sci, 69, 89-93.
- Rizzi L, Zaghini A, Roncada P (1998): Mycosorb Dossier. Altech (Reland) Ltd.
- Roberts BA, Patterson DSP (1976): Mycotoxins: detection of twelve mycotoxins in mixed animal feedstuffs. Using a novel membrane cleanup procedure. J Assoc Off Anal Chem, 58, 1178-1181.
- Rosa CA, Miazzo R, Magnoli C, Salvano M, Chiac SM, Ferrero S, Saenz M, Carvalho EC, Dalcero A (2001): Evaluation of the efficacy of bentonite from South of Argentina to ameliorate the toxic effectys of aflatoxin in broilers. Poult Sci, 80, 139-144.
- Salmanoğlu B (2002): Broyler civcivlerde aflatoksin B1’in karaciğerin A vitamini depolanması üzerine etkisi. Turk J Vet Anim Sci, 26, 1341-1344.
- Santurio JM, Mallmann CA, Rosa AP, Apel G, Heer A, Dagefode S, Bottcher M (1999): Effect of sodium bentonite on the performance and blood variables of broiler chickens intoxicated with aflatoxins. Br Poult Sci, 40, 115-119.
- Scheideler SE (1993): Effects of various types of aluminosilicates and aflatoxin B1 on aflatoxin toxicity, chick performance and mineral satatus. Poult Sci, 72, 282- 288.
- Shotwell OL, Hesseltine CW, Stubblefield RD, Sorenson WG (1966): Production of aflatoxin on rice. Appl Microbiol, 14, 425-428.
- Snedecor GW, Cochran WG (1980): Statistical Methods. 7th ed. The Iowa State University Press, Ames, Iowa, USA.
- Stanley VG, Ojo R, Woldesenbet S, Hutchinson DH, Kubena LF (1993): The use of Saccharomyces cerevisae to suppress the effects of aflatoxicosis in broiler chicks.
- Şanlı Y, Ceylan S, Kaya S (1982): Karma yemlerde aflatoksin analizi. Ankara Üniv Vet Fak Derg, 29, 50-70.
- Türk Standartları Enstitüsü (1991): Hayvan Yemleri- Metabolik (Çevrilebilir) Enerji Tayini (Kimyasal Metot). TSE No: 9610. Türk Standartları Enstitüsü, Ankara
Aflatoksin içeren rasyona katılan A vitamininin etlik civcivlerde performans, karaciğer ve dalak ağırlıkları ile tibia külü üzerine etkisi
Abstract
Araştırma aflatoksin içeren yemle beslenen etlik civcivlerde rasyona katılan A vitamininin canlı ağırlık, yemden yararlanma oranı, karaciğer ve dalak ağırlıkları ile tibia külü üzerine etkisini belirlemek amacı ile yapılmıştır. Çalışmada 84 adet günlük yaşta, Ross 308 etlik civciv kullanılmıştır. Her biri 28 civcivden oluşan bir kontrol ve iki deneme grubu olmak üzere üç ana grup halinde yürütülmüştür. Her bir ana grup 4 alt gruba bölünmüş ve gruplarda 7’şer civciv (4 erkek, 3 dişi) bulundurulmuştur. Kontrol grubuna aflatoksin ilave edilmeyen etlik civciv yemi verilirken, aflatoksin grubuna 2.5 ppm aflatoksin, aflatoksin+A vitamini grubuna 2.5 ppm aflatoksin ve 3866.66 IU A vitamini/kg yem ilave edilmiştir. Deneme 21 gün sürdürülmüştür. Çalışma sonunda kontrol, aflatoksin ve aflatoksin+A vitamini gruplarında canlı ağırlıklar sırasıyla 480.04 ± 15.76, 362.52 ± 16.06 ve 353.04 ± 16.06 g olarak tespit edilmiştir. Aflatoksin ve aflatoksin+A vitamini gruplarından elde edilen canlı ağırlık verileri kontrol grubuna göre önemli derecede (p<0.001) düşük bulunmuştur. Toplam yem tüketimi, aflatoksin içeren deneme gruplarında kontrol grubuna göre daha düşük (p<0.001) bulunmuştur. Ancak yemden yararlanma oranları bakımından gruplar arasında istatistik bakımdan önemli bir fark saptanmamıştır. Karaciğer ve dalak ağırlıkları; aflatoksin ve aflatoksin+A vitamini gruplarında kontrol grubuna göre daha yüksek (p<0.01) iken, kontrol grubunda tibia kül miktarı aflatoksin grubundan önemli derecede (p<0.05) yüksek, aflatoksin+A vitamini grubuna benzer bulunmuştur
Keywords
References
- Association of Offical Analytical Chemists (1990): Official Methods of Analysis of the Association of Official Analytical Chemist. 14th ed, Arlington, Virginia.
- Araba M, Wyatt RD (1991): Effects of sodium bentanite, hydrated sodium calcium aluminoslicate (Novasil) and ethacal on aflatoxicosis in broiler cfhicks. Poult Sci, 70, 6.
- Campbell TC, Hayes JR (1976): The role of aflatoxin metabolism in its toxic lesion. Toxicol Appl Pharmacol, 35, 199-222.
- Demet Ö, Oğuz H, Adıgüzel H (1995): Pirinçte aflatoksin üretimi. Vet Bil Derg, 11, 135-140.
- Dickson IR, Walls J, Webb S (1989): A vitamini and bone formation. Different responses to retinol and retinoic acid of chick bone cell in organ culture. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1013, 254-258.
- Edrington TS, Kubena LF, Harvey RB, Rottinghaus GE (1997): Influence of a superactivated charcoal on the toxic effects of aflatoxin or T-2 toxin in growing broilers.
- Ghosh RC, Chauhan HVS, Roy S (1990): Immunosuppression in broilers under experimental aflatoxicosis. Br Vet J, 146, 457-462.
- Harvey RB, Kubena LF, Elissalde MH, Philliıps TD (1993): Efficacy of zeolitic compounds on toxicity of aflatoxin to growing broiler chickens. Avian Dis, 37, 67- 93.
- Hoehler D, Marguardt RR (1996): Influence of vitamin E and C on the toxic effects of ochratoxin A and T-2 toxin in chicks. Poult Sci, 75, 1508-1515.
- Huff V E, Kubena LE, Harvey RB, Corrier DE, Mollenhauer HH (1986): Progression of aflatoxicosis in broiler chickens. Poult Sci, 65, 1891-1899.
- Jindal N, Mahipal SK, Mahajan NK (1994): Toxicity of aflatoxin B1 in broiler chicks and its reduction by activated chacoal, Res In Vet Sci, 56, 37-40.
- Kubena LF, Harvey RB, Bailey RH, Buckley SA, Rottinghaus GE (1998): Effects of a hydrated sodium calcium aluminosilicate T-BindTM on mycotoxicosis in young broiler chickens. Poult Sci, 78, 204-210.
- Kubena LF, Harvey RB, Phillips TD, Corrier DE (1990): Diminition of aflatoxicosis in growing chickens by the dietary addition of anhydrated sodium calcium aluminosilicate. Poult Sci, 69, 727-735.
- Larsson P, Tjalve H (1992): Binding of aflatoxin B1 metabolites in extrahepatic tissues in fetal and infant mice and in adult mice with depletion glutathione levels. Cancer Res, 52, 1267-1277.
- Ledoux DR, Rottinghaus GE, Bermudez AJ, Alanso- Debolt M (1999): Efficacy of hydrated sodium calsium aluminosilicate to ameliorate the toxic effects of aflatoxin in broiler chicks. Poult Sci, 78, 204-210.
- Liu XL, Zhou YZ (1989): The effect of aflatoxin B1 on A vitamini status and on microsomal mixed function oxidase in male Mouse. Zhonghua Yu Fang Yi Xue Za Zhi, 23, 218-221.
- Miazzo R, Rosa CA, De Queiroz Carvalho EC, Magnoli C, Chiacchiera SM, Palacio G, Saenz M, Kikot A, Basaldella E, Dalcero A (2000): Efficacy of synthetic zeolite to reduce the toxicity of aflatoxin in broiler chicks. Poult Sci, 79, 1-6.
- National Research Council (1994): Nutrient Requirements of Poultry. 9th review. National Academy Press, Washington, DC
- Nyandieka HS, Wakhisi J (1993): The impact of vitamins A, C, E, and selenium compound on prevention of liver cancer in rats. East Afr Med J, 70, 151-153.
- Oğuz H, Kurtoğlu V (2000): Effect of clinoptilolite on fattening performance of broiler chickens during experimental aflatoxicosis. Br Poult Sci, 41, 512-517.
- Oğuz H, Keçeci T, Birdane YO, Önder F, Kurtoğlu V (2000): Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during experimental aflatoxicosis. Res Vet Sci, 69, 89-93.
- Rizzi L, Zaghini A, Roncada P (1998): Mycosorb Dossier. Altech (Reland) Ltd.
- Roberts BA, Patterson DSP (1976): Mycotoxins: detection of twelve mycotoxins in mixed animal feedstuffs. Using a novel membrane cleanup procedure. J Assoc Off Anal Chem, 58, 1178-1181.
- Rosa CA, Miazzo R, Magnoli C, Salvano M, Chiac SM, Ferrero S, Saenz M, Carvalho EC, Dalcero A (2001): Evaluation of the efficacy of bentonite from South of Argentina to ameliorate the toxic effectys of aflatoxin in broilers. Poult Sci, 80, 139-144.
- Salmanoğlu B (2002): Broyler civcivlerde aflatoksin B1’in karaciğerin A vitamini depolanması üzerine etkisi. Turk J Vet Anim Sci, 26, 1341-1344.
- Santurio JM, Mallmann CA, Rosa AP, Apel G, Heer A, Dagefode S, Bottcher M (1999): Effect of sodium bentonite on the performance and blood variables of broiler chickens intoxicated with aflatoxins. Br Poult Sci, 40, 115-119.
- Scheideler SE (1993): Effects of various types of aluminosilicates and aflatoxin B1 on aflatoxin toxicity, chick performance and mineral satatus. Poult Sci, 72, 282- 288.
- Shotwell OL, Hesseltine CW, Stubblefield RD, Sorenson WG (1966): Production of aflatoxin on rice. Appl Microbiol, 14, 425-428.
- Snedecor GW, Cochran WG (1980): Statistical Methods. 7th ed. The Iowa State University Press, Ames, Iowa, USA.
- Stanley VG, Ojo R, Woldesenbet S, Hutchinson DH, Kubena LF (1993): The use of Saccharomyces cerevisae to suppress the effects of aflatoxicosis in broiler chicks.
- Şanlı Y, Ceylan S, Kaya S (1982): Karma yemlerde aflatoksin analizi. Ankara Üniv Vet Fak Derg, 29, 50-70.
- Türk Standartları Enstitüsü (1991): Hayvan Yemleri- Metabolik (Çevrilebilir) Enerji Tayini (Kimyasal Metot). TSE No: 9610. Türk Standartları Enstitüsü, Ankara
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 1, 2007 |
| Published in Issue | Year 2007 |